Als2cl (NM_001146059) Mouse Untagged Clone

CAT#: MC222811

Als2cl (untagged) - Mouse ALS2 C-terminal like (Als2cl), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Als2cl
• Synonyms: 79H19D; 79H19E; BC022645; D930044G19Rik; mRn.49018
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222811 representing NM_001146059
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAGCTCTGAGGAGGCAGACCTGCTCCGGCTGGAGGAGGTCTTCTCAACGACTCTTGCTCGAACCA
  TCAGTCTCATCCTGCAGCCTCTGCTCTTAGCTGACCCGGAGCCCTCAGATCCCTGTGGCAAAGAGTGCCT
  TCGGCTTCTGCAGCAGCTGCATGAGAGTGCCCAGCGGTTGTGGTATGTGACAGAGCAAAGCCTGCTTTCG
  CTGCGGCAGAGGCTATACCACCCACCTTCCAAGGGTCTAGAGGCCGTGCTGTTACTGAGCAATGCTGACC
  ATGTCCTACAAGCCCACATGGAATACATCAAGTCCTACACTGATTGCGTGGTGGCGCAAGCTTTTCAGAA
  GGTCTCAAAGAAGAGGAGTGAGTTCTGGAGGAGCCAGCGCAAGGCGCTGAGGCAGCTACTGTCCTCTGGG
  AACTCTGAGGGCTCTGTGGGCACCACGATGTGCCAGGCCCTCCGCCAGCCACTCTCCCAGCACGTGCAGA
  AATACTTGCTTCTCCTGCTGAGCCTCAGGGACACCCTTGATGAGAGCCACCCGGCCCAGGAGCTGGTGAT
  GCATGCTATCACCCTGTTTGGAAACCTGCAGTCCTTCATGGGACAGGCCTTGGACCAAGCTGTGGCCACA
  CAGGCCCTGTGGCACAGCCTCAGCAGCCGGCTGAGGGATGTGCTCTGCAGCCCTGCTCACCGACTCTTGC
  AGGACAGCCAGGACATCCCCGTGGTGGTCACTCCACTGCGGGCTGAGCGCGTGCTGCTCTTTGATGACTC
  GCTTGTCCTGTTGCAGGGCCACAATACCCACACGTTTGACCTGAAGTTGGTGTGGGTAAAACCCGGCCAA
  GACAAGTGTGTGCTTCATATCCTCACTCCTGAAGAAGAGATCTCCTTCTGCACCAGAGACCCCCAGGGCC
  AGGTTGTCTGGCAGTGGAAGGTGACCCAGGCTGTGTGCCAGGCCCTCTGTGGCAAGAAGGACTTCCCTGT
  GCTGGGATCAGGCCGGGAGACGTCTGTGCCTCCTGAATGTCGCTGTGTGGCCTATACCTTCCGCCGAGAG
  GGGCGGCTCTGCCAGGCTACCTATGATGGCGAGTGGTGTCGAGCTAAGCCCCATGGCAAGGGAACCCTGA
  AGTGGCCAGATGGGCGGAACCACGTGGGGACTTTTTACCAGGGCTTGGAGCATGGCTTTGGCATCTGCCT
  GGTGCCCCAGGCCTCTGAGGACAAGTTTGACTGTTACAAGTGCCACTGGCGGGAAGGCAGGATGTGTGAG
  TACGGCATCTGTGAGTATGGCACTGACGAGGTGTACAAGGGCTATTTTCAGGCCGGTCTGCGGCACGGGT
  TTGGCATCCTCGAGAGTGCCCCACAGGCTCCCCAGCCCTTCAGGTACACGGGCCACTGGGAGAGAGGCCA
  GAGGAGTGGCTACGGCATCGAGGAGGACAGGGACAGGGGCGAGCGCTACATTGGCATGTGGCAGGCTGAC
  CAGCGTCATGGCCCTGGGGTCGTGGTCACCCAGGCAGGCGTCTGCTATCAGGGCACATTCCAAGGGGACA
  AGATGGCTGGCCCAGGCATCCTGCTCTGCGAGGACGACTCTCTGTACGAAGGTACTTTTACCAGGGACCT
  GACGCTCCTAGGGAAGGGCAAGGTCACCTTCCCCAATGGTTTTACCTTGGATGGTTCTTTCAGCAGTGGG
  ACAGATAAAGGACTGTACACACAGGGAGTGCTGGACACGGCTGCCCTCCCACCTGACCCAAGCAGCACAC
  GCAAGAGACAGCTGGGACTGGGCACCTTCCCTGTGGAGAGCCGCTGGCAGGGAGTCTACAGCCCCTTCCG
  GGACTTCTTGCGTTTAGGCTGTCCTGGGGAACAGCAGGAAGCCCTCCTGGGCTTCCACACGCAGAGCTCC
  AGGGAGCTACGCAAGTCCCAGGAGTGCCTGTGTTGTGAGAGGAGCCACCCCGAGGACTGTGTGGGCAGCA
  TGGAAGATACCCTGAAGGAGCTGCTGCAGCACCGCAAGCCCAAGGCCCTGCAGCAGTACCTCAGGAAGGC
  TCTGAGCAATTCCCGACACCCCCTGGGGCAGCTGCTCCGCACCCTGATGCTGACCTTCCAGGCCACATAT
  TCAGGCATCGGGGCCAACAAACACCTGCAGGAGATGGCCCAGGAGGAGGTGAAGCAGCATGCCCGGGAGC
  TTTGGGCTGCCTACAGGGGTCTACTGAAGGTTGCCTTGCAGCGCCAGGGCCAGACCCTGGAGGAGGAGAA
  CATGGAGACCAGGGACCTGCAGGTGCACGGGTTGCTCTTGCCCCTCATCCTGCCCAGCTTCTACTCAGAG
  CTCTTCACACTGTACCTGCTTCTCCATGAGAGAGAGGATGGACTGTACAGCCGGGGCATCACCAACCTCA
  GCCTCTTCCCTGACACCAAGCTGCTGGAGTTCCTGGATGTGCAGAAGCATCTGTGGCCCCTCAAGGACCT
  CAAGCTGACAAGCAATCAGAGATATTCTCTGGTCCGGGACAAGTGTTTCCTGACGGCCACTGAGTGTCTG
  CAGAAGATCATCACCACAGTGCACCCTCGGGAGAAGCTGGAGGTACTTGAGAAGACCTATGGGGAAATTG
  AGGCCACGGTGTCACGGGTGCTGGGCTGCAAATACAAACTGCCCATGGATGACCTATTGCCACTGCTTAT
  CTATGTGGTGTCCCGAGCTCGAATTCAGCATCTGGGAGCTGAGATCCACCTGATCCGTGACATGATGGAT
  CCCGTCCACACAGGTGGCCTCCATGACTTCCTGCTGACAGCTTTAGAGTCCTGTTATGAGCACATCCAGA
  AGGAAGACATGAGGCTGCACCATCTGCCCGGGCACTGGGACGCTAGAGAACTCTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146059
• Insert Size: 2859 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146059.1, NP_001139531.1
• RefSeq Size: 5106 bp
• RefSeq ORF: 2859 bp
• Locus ID: 235633
• UniProt ID: Q60I26
• Cytogenetics: 9 60.79 cM
• Gene Summary: Acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase. Regulates the ALS2-mediated endosome dynamics (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.