Thrap3 (NM_146153) Mouse Untagged Clone

CAT#: MC222806

Thrap3 (untagged) - Mouse thyroid hormone receptor associated protein 3 (Thrap3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thrap3
• Synonyms: 9330151F09Rik; B230333E16Rik; Trap150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222806 representing NM_146153
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAACAAACAAATCCAAGTCTGGGTCTCGCTCTTCTCGCTCAAGATCTGCATCCAGATCTCGGT
  CTCGTTCGTTTTCAAAGTCTCGGTCCCGAAGCCGATCTGTCTCTCGTTCAAGGAAGCGCAGGCTGAGTTC
  TAGGTCTCGTTCCAGATCATATTCTCCAGCTCATAACAGAGAAAGGAATCACCCCAGAGTGTATCAGAAT
  CGAGATTTCCGAGGTCATAACAGAGGCTACAGGAGGCCCTATTACTTCCGTGGGCGAAACCGAGGCTTTT
  ATCCATGGGGCCAGTATAACCGAGGTGGCTATGGAAACTACCGTTCCAATTGGCAGAACTACCGGCAAGC
  ATACAGCCCTCGTCGGGGCCGTTCTCGATCCCGGTCCCCAAAGAGAAGGTCCCCTTCACCACGGTCCAGG
  AGCCATTCTAGGAACTCTGACAAGTCATCCTCTGACAGGTCAAGACGCTCCTCATCCTCCCGGTCGTCCT
  CCAACCACAGCAGAGTTGAGTCGTCTAAGCGAAAGTCTACAAAAGAGAAAAAGTCCTCTTCCAAGGATAG
  CCGGCCATCTCAGGCAGCTGGTGATAACCAGGGAGATGAGGCTAAGGAGCAGACATTCTCTGGAGGCACC
  TCTCAAGATATAAAAGGGTCTGAGAGCTCAAAGCCATGGCCAGATGCCACCACCTATGGCGCCGGTTCTG
  CATCACGGGCCTCGGTTTCTGATCTGAGTCCCCGGGAGAGAAGCCCAGCTCTCAAAAGCCCCCTCCAGTC
  TGTGGTGGTTAGGCGCAGGTCACCACGCCCTAGCCCTGTGCCAAAACCCAGTCCTCCACTTTCTAATGCG
  TCCCAGATGGGCTCGTCTATGTCAGGGGGTGCTGGGTATCAGTCTGGAGCACACCAAGGCCAGTTCGACC
  ATGGCTCTGGGTCTTTGAGTCCATCCAAAAAGAGCCCTGTGGGTAAGAGTCCACCAGCTACTGGCTCTGC
  ATATGGCTCATCTCAGAAAGAGGAGAGTGCTGCTTCGGGAGGGGCAGCATATTCAAAAAGGTATCTGGAG
  GAGCAGAAGACTGAGAATGGGAAGGATAAGGAGCAGAAACAAACAAATGCCGATAAGGAGAAGCTGAAGG
  AGAAAGGGGGCTTCTCTGATGCTGATGTCAAAATGAAATCTGATCCATTTGCTCCCAAGACGGACTCTGA
  GAAGCCCTTCCGAGGCAGCCAGTCGCCCAAAAGGTATAAGCTTCGGGATGACTTTGAGAAAAAGATGGCT
  GACTTCCACAAGGAGGAGCTGGATGAGCACGATAAGGACAAAAGTAAGGGAAGGAAGGAACCCGAGTTTG
  ATGATGAGCCCAAATTTATGTCGAAAGTCATAGCCGGTGCAAGCAAAAACCAGGAGGAAGAGAAGTCAGG
  CAAGTGGGAGAGCCTGCACACAGGGAAGGAAAAGCAGAGGAAGGCAGAGGAAATGGAGGATGAGCCTTTC
  ACAGAGAGATCCCGAAAGGAGGAGCGTGGAGGGTCCAAGAGGAGTGAAAGTGGGCACAGGGGCTTTGTGC
  CAGAAAAGAATTTCCGGGTGACTGCGTACAAGGCGGTCCAGGAGAAAAGTTCATCGCCGCCCCCAAGGAA
  GACCTCTGAGAGCCGTGACAAGCTGGGAAGCAAAGGAGACTTTTCCTCAGGGAAGTCTTCCTTTTCCATT
  ACCCGGGAGGCCCAAGTCAATGTCCGGATGGACTCCTTCGATGAGGACCTTGCACGACCTAGTGGTTTAT
  TGGCTCAGGAGCGAAAGCTCTGTCGGGATCTAGTCCATAGCAACAAAAAGGAGCAGGAGTTCCGTTCCAT
  TTTCCAGCACATACAGTCGGCTCAGTCTCAGCGTAGCCCCTCAGAACTGTTTGCTCAGCACATAGTGACC
  ATTGTTCATCATGTTAAAGAGCATCACTTTGGATCCTCTGGAATGACATTGCATGAACGCTTTACTAAAT
  ACCTAAAGAGAGGAAATGAACAAGAAGCAGCTAAAAATAAGAAAAGCCCAGAGATACACAGGAGGATAGA
  CATTTCCCCCAGTACATTCAGAAAGCATGGTTTGACTCATGAGGAATTGAAAAGTCCACGGGAACCTGGC
  TACAAGGCTGAGGGAAAATACAAAGATGATCCTGTTGATCTTCGCCTTGATATTGAACGTCGTAAAAAAC
  ATAAGGAGAGAGATCTTAAGCGAGGTAAATCAAGAGAGTCAGTGGATTCCAGAGACTCTAGCCACTCAAG
  AGAAAGATCAACTGAAAAGACAGAGAAAACCCACAAAGGATCAAAGAAGCAGAAGAAGCACCGCAGAGCA
  AGAGACCGGTCCAGGTCCTCCTCCTCCTCCTCCCAGTCCTCCCATTCCTACAAAGCAGAGGAGTACCCTG
  AGGAGGCAGAAGAGAGGGAGGAGAGCACCTCAGGTTTCGACAAATCCAGATTGGGGACCAAAGACTTCGT
  GGGTCCGAATGAAAGAGGCGGCAGAGCTCGGGGAACCTTTCAATTTCGTGCAAGAGGGAGAGGATGGGGC
  AGAGGAAACTATTCTGGTAACAATAACAACAACAGCAACAATGATTTTCAAAAGAGAAGCCGGGAAGAAG
  AGTGGGATCCAGAGTACACGCCCAAGAGCAAGAAGTATTACTTGCATGACGACCGGGAAGGCGAGGGCAG
  CGACAAGTGGATGGGCCGAGGCCGAGGCCGAGGAGCCTTTCCCCGGGGGCGGGGCCGGTTCATGTTCCGG
  AAGTCCAGTACCAGCCCCAAGTGGGCCCATGACAAGTTCAGTGGGGAGGAGGGAGAGATAGAAGACGACG
  AGAGTGGGACAGAGAACAGAGAAGAGAAGGACAGTTTACAGCCCTCAGCTGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146153
• Insert Size: 2856 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146153.3, NP_666265.3
• RefSeq Size: 4350 bp
• RefSeq ORF: 2856 bp
• Locus ID: 230753
• UniProt ID: Q569Z6
• Cytogenetics: 4 D2.2
• Gene Summary: Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-ARNTL/BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes (PubMed:24043798).[UniProtKB/Swiss-Prot Function]