Megf11 (NM_172522) Mouse Untagged Clone

CAT#: MC222803

Megf11 (untagged) - Mouse multiple EGF-like-domains 11 (Megf11), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Megf11
• Synonyms: 2410080H04Rik; D130061K05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222803 representing NM_172522
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGTCTGCGGTGGGCCTCCTTGTCTTCCTTCTGCAAGCTGCTCTTGCTCTGAACCCTGAAGACC
  CCAATGTGTGTAGCCACTGGGAGAGCTATGCCGTGACTGTGCAGGAGTCTTATGCACACCCCTTTGATCA
  GATCTACTACACACGATGTGCAGACATCCTCAACTGGTTCAAGTGTACCCGGCACCGGATCAGCTATAAG
  ACCGCGTATAGGCGCGGCCTCCGGACCATGTACCGGCGGAGGTCCCAATGCTGCCCTGGCTACTATGAGA
  ACGGAGACTTCTGCATTCGCTGTGACAGCGAGCACTGGGGTCCCCACTGCAGCAACCGGTGTCAGTGTCA
  GAACGGCGCCCTGTGCAACCCTATCACCGGCGCCTGCGTGTGCGCCCCGGGCTTCCGAGGCTGGCGCTGT
  GAGGAACTCTGCGCTCCTGGTACTCACGGCAAGGGCTGCCAGCTGCTCTGTCAGTGCCACCATGGCGCCA
  GCTGTGACCCGCGCACTGGCGAGTGCCTCTGCGCTCCTGGCTACACAGGCGTTTACTGTGAGGAGCTGTG
  CCCCCCTGGGAGCCATGGAGCTCACTGTGAGCTGCGCTGCCCCTGCCAGAATGGAGGCACCTGCCACCAC
  ATCACTGGCGAATGTGCCTGCCCTCCAGGCTGGACGGGAGCAGTGTGTGCCCAGCCCTGCCCTCCAGGGA
  CCTTTGGCCAGAACTGTAGCCAGGACTGTCCCTGCCACCATGGAGGCCAGTGTGACCATGTGACTGGACA
  ATGCCACTGTACAGCTGGATACATGGGGGACAGGTGTCAAGAAGAATGTCCCTTTGGAACGTTCGGTTTC
  CTGTGCTCTCAACGCTGTGACTGCCACAATGGAGGTCAATGTTCACCAGCCACAGGGGCCTGTGAGTGTG
  AGCCTGGCTACAAGGGCCCTAGCTGCCAGGAGCGGCTATGCCCTGAGGGCCTGCATGGCCCAGGCTGCAC
  CTTGCCCTGCCCCTGTGACACCGAGAACACTATCAGCTGCCATCCAGTTACTGGAGCTTGTACCTGCCAA
  CCAGGCTGGTCTGGCCACTACTGCAATGAGTCCTGCCCCGCCGGCTACTATGGCAACGGTTGCCAGCTAC
  CCTGCACCTGCCAGAACGGTGCTGACTGCCACAGTATCACCGGGAGCTGCACTTGTGCTCCAGGCTTCAT
  GGGAGAGGTGTGTGCCGTCCCCTGTGCTGCAGGGACCTATGGTCCCAACTGTTCATCTGTATGTAGCTGT
  AGCAACGGCGGCACCTGTTCCCCAGTGGATGGCTCCTGCACCTGCCGAGAGGGATGGCAGGGCCTGGACT
  GCTCCCTGCCTTGTCCCAGTGGGACCTGGGGCCTGAACTGCAATGAGACTTGCATCTGTGCCAATGGAGC
  TGCCTGCAGCCCCTTTGATGGGTCCTGTGCCTGCACCCCAGGCTGGCTGGGGGACTCCTGTGAACTGCCC
  TGCCCGGACGGCACTTTTGGGCTGAACTGCAGTGAGCATTGCGACTGCAGCCATGCTGATGGCTGTGACC
  CTGTCACAGGCCACTGCTGCTGCCTGGCAGGATGGACAGGCATCCGCTGTGATAGCACGTGTCCTCCAGG
  TCGCTGGGGCCCCAACTGTTCAGTGTCCTGCAGCTGTGAGAACGGAGGTTCCTGCTCCCCGGAGGACGGG
  AGCTGCGAGTGTGCCCCTGGCTTTCGAGGACCCTTATGTCAGAGAATCTGCCCACCAGGATTCTACGGCC
  ATGGCTGCGCCCAGCCTTGTCCCCTCTGCGTGCACAGCAGGGGGCCCTGCCACCACATCAGTGGTATCTG
  TGAGTGCCTGCCAGGATTCTCTGGAGCCTTGTGCAACCAAGTGTGTGCTGGAGGGCACTTCGGGCAGGAC
  TGTGCCCAGCTCTGTTCCTGTGCCAACAATGGGACCTGCAGCCCCATCGATGGCTCCTGTCAGTGCTTCC
  CTGGGTGGATTGGCAAGGACTGCTCACAGGGTTGCCCTTCAGCATTTTTTGGGAAGGACTGTGGGCACAT
  ATGCCAGTGTCAGAATGGAGCCAGCTGTGACCACATCACTGGGAAATGCACCTGTCGAACAGGCTTCTCT
  GGCCGCCACTGTGAACAGAGATGTGCCCCTGGAACCTTTGGATATGGGTGTCAGCAGCTATGTGAGTGCA
  TGAACAATGCCACTTGTGACCACGTCACTGGTACCTGTTACTGTAGCCCGGGATTCAAAGGAATCAGGTG
  TGACCAAGCTGCCCTCATGATGGATGAGCTGAATCCCTACACCAAGATCAGTCCAGCTCTGGGAGCAGAG
  CGGCACTCAGTGGGTGCTGTCACCGGCATCGTTCTCCTGTTGTTCCTGGTGGTGGTGCTGCTGGGCCTGT
  TTGCCTGGCGACGGAGGCGGCAGAAAGAGAAAGGCCGTGACCTGGCTCCCCGAGTCTCCTACACCCCAGC
  CATGAGGATGACCAGCACAGACTACTCTCTCTCAGATTTGTCTCAAAGTAGCAGCCATGCCCAGTGCTTT
  TCCAATGCCAGCTACCACACACTGGCGTGTGGGGGGCCTGCCACCAGCCAGGCCAGCACTCTGGACAGGA
  ACAGCCCCACCAAGCTCAGTAACAAGTCCCTTGACAGAGACACAGCAGGCTGGACCCCCTACAGCTATGT
  GAACGTGTTAGACTCCCATTTCCAGATCAGTGCCCTGGAGGCCAGGTACCCGCCCGAGGACTTCTACATT
  GAACTTAGACACCTCAGCCGCCATGCTGAGCCACACTCACCAGGCACTTGTGGAATGGACAGACGTCAGA
  ACACATACATTATGGACAAAGGCTTCAAAGTTGCACCTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172522
• Insert Size: 2844 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172522.4, NP_766110.3
• RefSeq Size: 3669 bp
• RefSeq ORF: 2844 bp
• Locus ID: 214058
• UniProt ID: Q80T91
• Cytogenetics: 9 C
• Gene Summary: May regulate the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. Mosaics provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' coding region, 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C terminus and is shorter than isoform 1. CCDS Note: This CCDS representation is based on the splice pattern of AK051642.1, which contains an alternative and shorter 3' end compared to homologous transcripts, including human NM_032445.2, AB300051.1 and BC126313.1. Mouse AK122555.2 encodes a longer protein, as represented in SwissProt Q80T91.3 (1091 aa), but this mRNA may possibly retain an intron at its 3' end (nts 3409-4001) compared to mouse AK160406.1 and homologous human transcripts, and thus, more evidence is needed to represent this longer protein.