Osbpl1a (NM_207530) Mouse Untagged Clone

CAT#: MC222783

Osbpl1a (untagged) - Mouse oxysterol binding protein-like 1A (Osbpl1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Osbpl1a
• Synonyms: G430090F17Rik; Gm753; ORP-1; Osbpl1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222783 representing NM_207530
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACAGAAGCAGAACAGCAGCTTCTCCATCATGCCAGAAATGGCAATGCTGAAGAGGTGCGAAAGC
  TGCTGGCAGCCATGGCACGGATGGAAGTGGTCGCTGACATCGACTGTAAAGGAAGAAGTAAGTCTAACTT
  GGGCTGGACACCTCTTCACCTGGCATGCTATTTTGGACACAAACAAGTGGTCGAGGATCTGTTGAAGGCT
  GGTGCAAAAGTGAACATGTTAAACGACATGGGGGACACGCCACTGCATCGAGCCGCCTTCACAGGACGAA
  AGGAGCTGGTCTTGCTTCTTTTAGAATACGATGCTGACAGTACAGTTGTAAATGGAAGTGGACAGACAGC
  AAAAGAAGCCACCCATGACAAAGAAATCAGAAACATGCTTGAAGCTGTGGAGAGGACTCAACAAAGAAAG
  CTTGAAGAATTGCTTTTAGGAGCAGCTAGAGAAGGAAGAACAGCCGAAGTATCAGCTCTGCTCAGCAGGC
  CCAATCCTCCTGATGTGAACTGCTCAGATCAGCTAGGAAATACCCCCTTACACTGTGCCGCCTACCGGGC
  CCACAAGCAGTGTGTCCTAAAGCTCCTGAGAAGCGGAGCTGACCCCAGCCTGAAGAACAAGAACGATCAG
  AAACCTCTTGACCTTGCCCAGGGTGCTGAAATGAAGCATATTCTTGTTGGTAATAAGGTTGTCCACAAAG
  CACTGAAACGCTACGAAGGCCCTCTCTGGAAGAGTTCAAGATTTTTTGGCTGGAAGTTATTTTGGGTAGT
  GTTAGAACATGGAGTCCTTTCCTGGTATAGGAAGCAGCCTGATGCAGTCCATAATTCTTATCGCCAAGGA
  TGCAAACACCTGACTCAAGCCGTGTGCACGGTGAAACCTACAGATAGCTGCCTCTTCTCCATTCGATGCT
  TTGATGACACTGTTCACTGCTTCAGGGTTCCTAAGAACAGCGTACAGCAATCAAGAGAGAAGTGGCTGGA
  AGCAATTGAAGAACACTCCGCATACAGCACTCACTACTGTTCCCAGGATCAGGTGACTGATGATGAAGAA
  GAAGATGTAGTTTCGGCCATGGACTTGAAGGAGTCGTTAGCGAGAGCACAAACATGTCAACAGAGACTAG
  ATAGGGAAATTTACAACTTTCTCAAAATGATTAAGGAGTGTGATGTGGCCAAAGATATGCTTCCATCGTT
  TCTTCAGAAAGCTGATATTGTCTCCGAAGCTTCTAGAGAGACTTGTGTGGCGTTGAATGACTGTCTTAAT
  CTCTTCACTAAACAGGAAGGGGTGAGGAATTTTAAATTGGAACAGGAGCAAGAAAAAAACAAAATTTTGT
  CAGAAGCACTGGAGACTTTAGCCACTGAGCATCATGAACTAGAACGATCCCTGGTTGAAGGATCCCCGCC
  TGTCAGCATCCTTAGTGAGGAGGAGTTCTATGATGCACTGTCAGGTTCCGAGTCCGAGGGGTCGCTGACT
  TGCCTGGAAGCTGTGACAGCACACTCCTTTGAAGAGAACGAAGTGCCTGGGAGCAGCGGAAAGCACAGAA
  TGTCTGAAGGAAAAGACTGTGGTGGGGGAGATGCGCTCTCCAATGGCATCAAAAAGCACAGAACAAGCCT
  GCCATCACCTATGTTCTCCAGAAACGATTTCAGCATCTGGAGCATCCTCAGAAAATGCATTGGGATGGAA
  CTGTCCAAGATCACAATGCCAGTGATATTTAATGAGCCTCTGAGCTTCCTGCAGCGGCTAACTGAATACA
  TGGAGCACACGTACCTCATCCACAAAGCCAGTTCACTTTCTGATCCTGTGGAAAGGATGCAGTGTGTGGC
  TGCATTTGCTGTGTCTGCTGTCGCCTCTCAGTGGGAGCGCACCGGAAAGCCCTTCAACCCGCTTCTGGGA
  GAGACTTATGAATTAGTTCGAGATGACCTTGGGTTTAGGCTCATCTCAGAACAGGTCAGCCATCATCCCC
  CAATCAGTGCATTCCATGCAGAAGGGCTCAACAATGACTTCATCTTCCATGGCTCAATTTACCCCAAACT
  GAAGTTCTGGGGCAAGAGTGTAGAAGCAGAGCCTAAAGGAACCATCACCTTGGAGCTTTTAGACCACAAC
  GAAGCATACACATGGACAAACCCCACCTGTTGTGTGCATAACATCATCGTGGGCAAGCTCTGGATTGAAC
  AGTATGGCAACGTGGAAATCATAAACCACAAGACTGGGGACAAATGTGTGCTGAATTTTAAGCCATGTGG
  TCTTTTTGGCAAGGAATTACACAAAGTTGAAGGCTACATACAAGATAAAAGCAAAAAGAAGCTCTGTGCT
  CTCTATGGGAAGTGGACTGAGTGCTTGTACAGCGTAGACCCTGCCACTTTTGATGCTTACAAAAAAAATG
  ATAAGAAAAACACAGAAGAGAAGAAGAACAGCAAACAGACAAGCTCATCTGAGGAGTCTGATGAAATGCC
  AGTGCCAGATTCCGAGAGCGTATTCATTATCCCTGGAAGTGTGCTCCTGTGGCGGATAGCCCCGCGGCCT
  CCCAACTCTGCTCAGATGTATAACTTTACCAGCTTTGCCATGGTTTTGAATGAAGTCGACAAGGAGATGG
  AGAGTGTGATTCCCAAGACTGACTGCAGGCTACGGCCTGACATCAGAGCCATGGAGAACGGAGAGATAGA
  TCTAGCTAGTGAAGAAAAGAAACGGCTTGAGGAAAAGCAAAGAGCAGCCCGCAAGAACAGGTCCAAGTCG
  GAAGAGGACTGGAAGACAAGGTGGTTCCATCAAGGTCCTAACCCCTACAGTGGAGCACAGGACTGGATTT
  ATTCTGGCAGCTACTGGGACAGAAACTACTTCAATTTGCCTGATATTTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207530
• Insert Size: 2853 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207530.3, NP_997413.2
• RefSeq Size: 3958 bp
• RefSeq ORF: 2853 bp
• Locus ID: 64291
• UniProt ID: Q91XL9
• Cytogenetics: 18 A1
• Gene Summary: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.