Inpp4a (NM_030266) Mouse Untagged Clone

CAT#: MC222757

Inpp4a (untagged) - Mouse inositol polyphosphate-4-phosphatase, type I (Inpp4a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Inpp4a
• Synonyms: 107kDa; 9630012D15; D130048C09Rik; R74740
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222757 representing NM_030266
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAAGAGAGCACAGCCCTCGCCATGGTGCCAGGGCCCGTGCGATGCAGCGGGCTTCCACCATTG
  ACGTGGCAGCCGACATGGTTGGCCTCTCTTTGGCAGGAAATATCCAAGATCCAGATGAGCCCATTTTAGA
  ATTCAGCTTAGCTTGCAGTGAGCTGCACACTCCATCGCTAGATCGAAAACCAAATAGTTTTGTGGCTGTG
  AGTGTCACCACCCCTCCGCAGGCGTTCTGGACGAAGCACGCACAGACGGAGATCATCGAGGGAACCAACA
  ACCCTATCTTTCTAAGCAGCATTGCCTTCTTTCAAGACTCCCTTATCAATCAGATGACCCAGATCAAGCT
  GTCTGTGTATGATGTCAAAGACAGATCTCAGGGAACAATGTACTTACTGGGCTCTGGAACATTCGTGGTC
  AAAGATCTGCTCCAGGACAGGCATCACAGATTGCATCTGACACTGAGGTCTGCAGAGAGTGACCGTGTCG
  GCAACATAACTGTGATCGGCTGGCAGATGGAGGAGAAGTCCGACCAGCAGCCCCCTGTGACCCGGTCTCT
  GGACACTGTCAATGGAAGGATGGTTCTGCCCGTTGATGAAAGCTTGACTGAGGCCTTGGGAATCCGATCC
  AAATATGCTTCTTTGCGAAAAGACAGCTTACTGAAAGCAGTGTTTGGTGGTGCCATCTGCCGCATGTACC
  GCTTCCCAACTACTGATGGTAACCACCTACGGATCCTGGAGCAGATGGCAGAGAGCGTCCTCTCCCTGCA
  CGTGCCTCGGCAGTTTGTGAAGCTACTGCTGGAAGAAGATGCAGCCAGGGTATGTGAGTTGGAAGAGTTG
  GGGGAGCTGTCCCCTTGCTGGGAGAGCCTCCGGCGCCAGATTGTCACCCAGTATCAGACTATCATCCTCA
  CCTACCAGGAGAATCTGACTGACCTCCATCAGTACAAAGGTCCTTCCTTTAAAGCAAGCAGTTTGAAAGC
  AGATAAAAAGTTAGAATTTGTTCCCACAAACCTGCACATACAACGGATGCGAGTTCAAGATGATGGAGGC
  TCAGATCAGAACTACGATGTCGTCACTATTGGAGCCCCAGCAGCACACTGCCAAGGTTTTAAGTCAGGAG
  GTCTTCGAAAAAAGCTGCACAAGTTTGAAGAGGCCAAGAAACACAGTTTTGAGGAGTGTTGTACATCTTC
  TAGTGGCCAGTCCATCATCTACATACCACAGGACATCACCCGAGCCAAGGAGATCATTGCCCAGATCAAC
  ACCCTAAAAACCCAGGTCAGCTACTACGCGGAACGGCTCTCAAGGGCTGCGAAGGACAGGTCAGCTACTG
  GCCTTGAGAGGACTCTCGCCATCTTGGCAGACAAGACTCGGCAGTTGGTGACTGTCTGTGACTGTAAGCT
  GCTGGCCAACTCCATCCATGGGCTGAATGCAGCTCGGCCCGACTACATCGCTTCCAAGGCCTCCCCTACC
  TCGACTGAGGAGGAGCAGGTGATGCTTCGGAATGACCAGGACACCCTGATGGCCAGGTGGGCGGGGAGAA
  GCAGCCGGTCTTCCCTGCAGGTGGACTGGCATGAGGAGGAGTGGGAGAAAGTGTGGCTGAATGTGGACAA
  GAGCCTGGAGTGCATCATTCAGCGGGTGGACAAGCTGCTGCAGAAGGAGCGTCTGCATGGGGAGGGCTGC
  GAGGATGCTTTCCCCTGTTCGAGCACCTGCTCCAGCAAGAAAGATTGCAGCCCCCCTCCTGAAGAGTCCA
  GTCCAGGTGAGTGGAGCGAGGCCCTTTACCCTCTGCTGACCACCCTCACAGACTGTGTGGCCATGATGAG
  CGACAAGGCCAAGGCAGCCATGGTCTTCCTGCTCATGCAGGACAGTGCCCCCACCATTGCCTCGTACCTC
  AGCCTGCAGTATCGCCGTGACGTCGTCTTCTGCCAAACCCTGACAGCCCTCATCTGTGGCTTTATCATCA
  AGCTGAGGAACTGCCTGCACGACGGTGGCTTCCTGCGGCAGCTCTACACCATCGGCCTTCTGGCCCAGTT
  TGAGAGCCTGCTGAGCACCTACGGAGAGGAGTTGGCCATGTTGGAGGACATGAGCCTTGGAATCATGGAC
  CTGAGGAATGTGACCTTTAAAGTCACTCAGGCCACTTCGAATGCATCAAATGACATGCTGCCGGTCATCA
  CAGGAAACCGGGATGGCTTTAACGTGCGGATCCCTCTGCCAGGCCCACTGTTTGACTCCCTCCCCAGAGA
  GATCCAGAGTGGCATGCTGCTGCGGGTGCAGCCTGTCCTCTTCAACGTGGGCATCAATGAGCAACAGACA
  CTGGCTGAGAGGTTTGGAGATACGTCCTTACAAGAAGTTATCAATGTGGAGAGCTTGGTGCGGTTAAATT
  CCTACTTTGAGCAGTTTAAGGAGGTTTTGCCGGAGGACTGTCTACCTCGATCTCGGAGTCAGACCTGCCT
  TCCAGAGCTGCTGCGGTTCCTGGGACAGAATGTCCATGCACGCAAGAATAAGAATGTGGACATCCTCTGG
  CAAGCTGCTGAGGTCTGTCGCCGCCTTAACGGGGTCCGATTCACCAGCTGCAAGAGTGCCAAGGACCGCA
  CAGCCATGTCGGTGACCCTGGAGCAGTGTCTGATCCTGCAGCACGAGCACGGCATGGCCCCGCAGGTCTT
  CACACAAGCCCTGGAGTGCATGCGCAGTGAGGGTTGTCGGCGAGAAAACACAATGAAGAATGTTGGAAGT
  CGCAAATATGCATTTAACTCCCTGCAGCTGAAGGCCTTCCCCAAGCATTACAGGCCTCCAGAAGGGACTT
  ACGGAAAAGTTGAGACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030266
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030266.4, NP_084542.2
• RefSeq Size: 5748 bp
• RefSeq ORF: 2820 bp
• Locus ID: 269180
• UniProt ID: Q9EPW0
• Cytogenetics: 1 15.46 cM
• Gene Summary: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate. Involved in the regulation of megakaryocyte and fibroblast proliferation. Regulates cell growth downstream of transcription factor GATA-1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses several alternate in-frame splice sites in the coding region, compared to variant 3. The encoded protein (isoform 1) is shorter than isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.