Gabbr2 (NM_001081141) Mouse Untagged Clone

CAT#: MC222756

Gabbr2 (untagged) - Mouse gamma-aminobutyric acid (GABA) B receptor, 2 (Gabbr2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabbr2
• Synonyms: GABABR2; Gb2; Gm425; Gpr51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222756 representing NM_001081141
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCCCGCCGAGCTCCGGGCAGCCCCGGCCGCCGCCGCCACCGCCGCCGCCCGCGCGCCTGCTGC
  TGCCCCTGCTGCTGTCGTTGCTGCTGTCGTTGGCTCCCGGGGCCTGGGGCTGGGCGCGGGGCGCCCCCCG
  GCCGCCGCCCAGCAGCCCGCCGCTCTCCATCATGGGCCTCATGCCGCTCACCAAGGAGGTGGCCAAGGGC
  AGCATCGGGCGCGGCGTGCTCCCCGCCGTGGAGCTAGCCATCGAGCAGATCCGCAACGAGTCACTCCTGC
  GCCCCTACTTCCTGGACCTGCGACTCTACGACACCGAGTGTGACAATGCAAAGGGACTGAAAGCCTTCTA
  TGATGCAATAAAGTATGGGCCGAACCATTTGATGGTGTTTGGAGGCGTCTGTCCGTCTGTCACCTCTATC
  ATCGCGGAATCCCTCCAAGGCTGGAACCTGGTGCAGCTTTCCTTCGCCGCCACCACGCCTGTTCTTGCGG
  ATAAGAAGAAGTACCCATATTTCTTCCGGACGGTGCCCTCAGACAACGCGGTGAACCCGGCAATCCTGAA
  GCTGCTGAAGCACTTCCGCTGGCGGCGCGTGGGCACACTCACGCAGGACGTGCAGCGCTTCTCCGAGGTG
  AGGAACGACCTGACTGGGGTTCTGTACGGGGAAGACATTGAGATCTCAGACACGGAGAGCTTCTCCAATG
  ATCCCTGCACCAGCGTCAAAAAGCTCAAGGGGAACGACGTGCGGATCATCCTTGGCCAGTTTGACCAGAA
  CATGGCAGCGAAAGTCTTCTGTTGTGCCTTTGAGGAGAGCATGTTTGGCAGCAAGTACCAGTGGATCATC
  CCGGGCTGGTACGAGCCTGCGTGGTGGGAACAGGTGCACGTGGAGGCCAACTCCTCACGCTGCCTGCGCA
  GGAGCCTCCTGGCTGCCATGGAAGGCTACATCGGAGTGGACTTCGAGCCCCTGAGCTCCAAACAAATCAA
  GACCATCTCAGGGAAGACTCCACAGCAATACGAAAGAGAATACAACAGCAAGCGTTCGGGTGTAGGGCCC
  AGCAAGTTCCATGGGTATGCCTACGATGGCATCTGGGTCATCGCCAAGACCCTGCAGAGGGCCATGGAGA
  CACTGCATGCCAGTAGCAGGCACCAGCGGATCCAGGACTTCAACTACACAGACCACACCCTGGGCAGAAT
  CATCCTCAACGCCATGAACGAAACCAACTTCTTCGGAGTCACGGGTCAAGTTGTGTTCCGGAACGGGGAA
  AGAATGGGAACCATTAAATTTACTCAATTTCAAGACAGCAGAGAGGTGAAGGTCGGCGAGTACAACGCTG
  TGGCTGACACACTGGAGATCATCAACGACACCATAAGGTTCCAGGGGTCGGAGCCACCCAAGGACAAGAC
  CATCATTCTAGAGCAGCTTCGGAAGATCTCGCTTCCACTGTATAGCATCCTGTCAGCTCTCACTATCCTC
  GGCATGATCATGGCCAGCGCCTTCCTCTTCTTCAACATCAAGAACCGGAACCAAAAGCTGATCAAGATGT
  CAAGCCCCTATATGAACAACCTCATCATCCTGGGTGGGATGCTGTCCTATGCATCCATCTTCCTCTTTGG
  TCTCGATGGATCCTTCGTCTCAGAAAAGACCTTTGAAACACTCTGCACGGTCCGGACCTGGATTCTCACC
  GTGGGCTACACAACCGCCTTTGGAGCCATGTTTGCAAAGACCTGGAGGGTCCATGCCATCTTCAAAAATG
  TGAAAATGAAGAAGAAGATCATCAAAGACCAGAAGCTGCTCGTGATTGTGGGGGGCATGCTGCTGATCGA
  TCTGTGCATCCTGATCTGCTGGCAGGCTGTGGACCCCCTGCGGAGGACAGTGGAGCGGTACAGCATGGAG
  CCGGACCCAGCAGGACGGGACATCTCCATCCGCCCACTGCTGGAACACTGCGAAAACACCCACATGACCA
  TCTGGCTCGGCATTGTCTACGCCTACAAGGGGCTCCTCATGTTGTTCGGTTGTTTCTTAGCATGGGAAAC
  CCGCAACGTGAGCATCCCTGCCCTCAACGACAGCAAGTACATTGGGATGAGTGTGTACAATGTGGGGATC
  ATGTGCATCATCGGGGCCGCCGTCTCCTTCCTGACACGTGACCAGCCCAACGTGCAGTTCTGCATCGTGG
  CCCTGGTCATCATCTTCTGTAGCACCATCACTCTCTGCCTGGTGTTTGTGCCAAAGCTCATCACTCTGAG
  GACAAACCCTGACGCAGCCACTCAGAACAGGCGATTCCAGTTCACACAGAACCAGAAGAAAGAAGATTCG
  AAGACCTCCACCTCAGTCACCAGCGTGAACCAGGCGAGCACGTCACGCCTGGAGGGACTGCAGTCAGAAA
  ACCACCGCCTACGAATGAAGATCACAGAGCTGGACAAAGACTTGGAAGAAGTCACCATGCAGCTACAAGA
  CACACCAGAGAAGACCACATACATCAAACAGAATCACTACCAAGAGCTCAACGACATCCTCAGCTTGGGC
  AACTTCACAGAGAGCACAGATGGAGGAAAGGCCATTCTAAAAAATCACCTCGATCAAAACCCTCAGCTCC
  AGTGGAACACGACCGAGCCCTCAAGAACATGCAAAGACCCCATAGAGGACATCAACTCCCCAGAGCACAT
  CCAGCGCCGGCTGTCCCTCCAGCTCCCCATCCTCCACCACGCCTACCTCCCGTCCATTGGAGGCGTGGAC
  GCCAGCTGCGTCAGCCCCTGTGTCAGCCCTACAGCCAGCCCTCGCCACAGACACGTACCACCCTCCTTCC
  GAGTCATGGTCTCGGGCCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081141
• Insert Size: 2823 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081141.1, NP_001074610.1
• RefSeq Size: 3998 bp
• RefSeq ORF: 2823 bp
• Locus ID: 242425
• UniProt ID: Q80T41
• Cytogenetics: 4 B1
• Gene Summary: Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:10075644). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (By similarity). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).[UniProtKB/Swiss-Prot Function]