Cblb (NM_001033238) Mouse Untagged Clone

CAT#: MC222751

Cblb (untagged) - Mouse Casitas B-lineage lymphoma b (Cblb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cblb
• Synonyms: AI429560; AI851073; Cbl-b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222751 representing NM_001033238
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAATTCTATGAATGGCAGAAATCCTGGTGGTCGAGGAGGAAACCCCCGCAAAGGTCGCATTTTGG
  GGATTATTGATGCTATTCAGGATGCAGTCGGACCCCCAAAGCAAGCTGCAGCTGACCGCAGGACTGTGGA
  GAAGACTTGGAAACTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAGCTTCAGTTGAAAAACAGC
  CCACCGTATATACTTGATATTTTACCTGATACGTATCAGCACTTGAGACTTATATTGAGTAAATATGATG
  ACAACCAGAAGCTGGCTCAACTGAGCGAGAATGAGTACTTTAAAATCTACATCGATAGTCTCATGAAGAA
  GTCGAAGCGAGCGATCCGGCTCTTTAAAGAAGGCAAGGAAAGGATGTACGAAGAGCAGTCGCAGGACAGA
  CGGAATCTCACAAAGCTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAGGCGATCTTTCCCAATG
  GCCAGTTCCAGGGAGATAACTTCCGGATCACCAAAGCAGATGCTGCTGAGTTCTGGAGGAAGTTTTTTGG
  AGACAAAACTATTGTACCATGGAAAGTCTTCAGACAGTGCCTGCATGAGGTCCATCAGATCAGCTCTGGC
  CTGGAAGCAATGGCTCTGAAGTCAACCATTGATTTAACTTGCAATGATTACATCTCAGTGTTTGAATTTG
  ATATTTTTACCAGGCTATTTCAGCCCTGGGGCTCTATTTTACGGAATTGGAACTTCTTGGCTGTGACTCA
  CCCGGGATACATGGCATTTCTCACGTATGATGAAGTTAAAGCTCGGCTACAGAAATACAGCACCAAGCCT
  GGAAGTTACATTTTCCGGTTAAGCTGCACTCGGCTGGGACAATGGGCCATTGGCTATGTGACTGGGGACG
  GCAATATCCTACAGACCATACCTCATAACAAGCCCCTGTTCCAAGCCCTGATTGATGGTAGCAGGGAAGG
  CTTTTATCTTTATCCTGATGGGCGGAGCTATAACCCTGATTTAACCGGATTATGTGAACCTACACCTCAC
  GATCATATAAAAGTTACACAGGAGCAGTATGAACTGTATTGTGAAATGGGCTCCACTTTTCAGCTCTGCA
  AGATCTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGCGGGCATCTGATGTGCACTTCGTGCCT
  TACCGCGTGGCAGGAGTCTGATGGCCAAGGCTGCCCCTTCTGTCGCTGTGAGATAAAAGGAACGGAGCCC
  ATCATTGTGGACCCCTTCGACCCCAGAGATGAAGGCTCCAGGTGCTGCAGCATCATTGACCCTTTCAGCA
  TCCCCATGCTTGACTTGGACGATGACGATGATCGAGAGGAGTCTTTGATGATGAACAGGCTGGCGAGTGT
  TCGAAAGTGCACGGACAGGCAGAACTCACCAGTCACATCGCCAGGCTCCTCACCCCTTGCCCAGAGAAGA
  AAGCCTCAGCCAGACCCTCTCCAGATCCCCCACCTCAGCCTGCCACCAGTGCCTCCCCGCCTAGATCTCA
  TTCAGAAAGGCATCGTGCGCTCTCCGTGTGGCAGCCCCACAGGCTCGCCAAAGTCTTCTCCATGCATGGT
  TAGAAAACAAGACAAACCACTCCCGGCACCCCCTCCTCCCTTGAGAGATCCACCTCCTCCACCAGAGAGG
  CCTCCCCCCATCCCACCTGACAATAGACTGAGCAGACACTTCCACCATGGAGAGAGTGTGCCTTCCCGGG
  ACCAGCCCATGCCCCTCGAAGCCTGGTGCCCTCGGGATGCCTTCGGGACTAACCAGGTGATGGGATGTCG
  CATCCTCGGGGATGGCTCTCCAAAGCCTGGCGTCACCGCAAACTCCAGCTTAAATGGGAGGCACAGTAGA
  ATGGGCTCCGAGCAGGTTCTTATGAGGAAACACAGACGCCATGATTTGCCTTCAGAAGGAGCCAAGGTCT
  TTTCCAATGGCCACCTTGCCACTGAAGAATACGATGTCCCTCCTCGGCTTTCCCCTCCGCCTCCAGTCAC
  CACCCTTCTCCCAAGCATAAAGTGTACTGGTCCATTAGCAAATTGTCTCTCAGAGAAAACTAGAGACACG
  GTAGAAGATGATGACGATGAATACAAGATTCCTTCATCCCATCCTGTGTCCCTGAATTCACAACCATCTC
  ATTGCCATAATGTCAAAGCTCCTGTTCGGTCTTGTGATAATGGTCATTGTATATTGAATGGAACTCATGG
  TGCGCCTTCAGAGATGAAGAAATCGAACATCCCAGATTTAGGCATCTATTTGAAGGGTGAGGACGCCTTT
  GATGCTCTCCCTCCATCCCTCCCTCCTCCCCCACCTCCTGCAAGACACAGTCTCATCGAACATTCAAAAC
  CTCCAGGCTCCAGTAGCCGGCCTTCCTCAGGGCAGGACCTTTTCCTTCTTCCTTCAGATCCCTTTTTTGA
  CCCAACAAGTGGCCAAGTTCCATTGCCTCCTGCCAGGAGAGCAGCAGGAGACAGCGGCAAAGCCAACAGA
  GCCTCGCAGGACTATGACCAGCTCCCTTCATCTTCCGATGGTTCGCAAGCACCAGCTAGACCCCCCAAAC
  CACGACCCCGAAGGACTGCACCAGAAATTCATCACAGAAAGCCCCATGGGCCCGAAGCGGCGTTGGAAAA
  TGTCGATGCAAAAATTGCAAAACTCATGGGAGAGGGGTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAG
  ATTGCCCAGAATAACGTGGAAGTGGCCAGGAGCATACTTCGAGAATTTGCCTTCCCCCCTCCTGTCTCCC
  CACGTCTGAATCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033238
• Insert Size: 2817 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033238.1, NP_001028410.1
• RefSeq Size: 6323 bp
• RefSeq ORF: 2817 bp
• Locus ID: 208650
• UniProt ID: Q3TTA7
• Cytogenetics: 16 B5
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (PubMed:29237719).[UniProtKB/Swiss-Prot Function]