Grin1 (NM_008169) Mouse Untagged Clone

CAT#: MC222731

Grin1 (untagged) - Mouse glutamate receptor, ionotropic, NMDA1 (zeta 1) (Grin1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grin1
• Synonyms: GluN1; GluRdelta1; GluRzeta1; M100174; NMD-R1; Nmdar; NMDAR1; NR1; Rgsc174
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222731 representing NM_008169
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCATGCACCTGCTGACATTCGCCCTGCTTTTCTCCTGCTCCTTCGCCCGCGCTGCCTGCGACC
  CCAAGATTGTCAACATCGGCGCGGTGCTGAGCACGCGCAAGCACGAGCAGATGTTCCGCGAGGCAGTAAA
  CCAGGCCAATAAGCGACACGGCTCTTGGAAGATACAGCTCAACGCCACTTCTGTCACCCACAAGCCCAAC
  GCCATACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGTC
  ACCCGCCTACTCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCTGGCTTCTACAGAATCCC
  CGTCCTGGGGCTGACTACCCGAATGTCCATCTACTCTGACAAGAGCATCCACCTGAGCTTCCTTCGCACC
  GTACCACCCTACTCCCACCAGTCCAGCGTCTGGTTTGAGATGATGCGCGTCTACAACTGGAACCATATCA
  TCCTGCTGGTCAGCGATGACCACGAGGGCCGGGCAGCGCAGAAGCGCCTGGAGACGTTGCTGGAGGAGCG
  TGAGTCCAAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGAACCAAGAATGTGACGGCTCTGCTGATGGAA
  GCCCGGGACCTGGAAGCCCGGGTCATCATCCTTTCTGCAAGCGAGGACGACGCTGCCACCGTATACCGCG
  CAGCCGCGATGCTGAACATGACTGGCTCTGGGTACGTGTGGCTCGTCGGGGAGCGCGAGATCTCTGGGAA
  TGCCCTGCGCTACGCTCCTGACGGCATCATCGGACTTCAGCTAATCAACGGCAAGAACGAGTCGGCCCAC
  ATCAGTGACGCTGTGGGCGTGGTGGCACAGGCAGTCCACGAGCTCCTAGAAAAGGAGAACATCACTGATC
  CACCGCGGGGTTGCGTGGGCAACACCAACATCTGGAAGACAGGACCACTGTTCAAGAGGGTGCTGATGTC
  TTCCAAGTATGCAGATGGAGTGACTGGCCGTGTGGAATTCAATGAGGATGGGGACCGGAAGTTTGCCAAC
  TATAGTATCATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGTACCCATGTCATCC
  CAAATGACAGGAAGATCATCTGGCCAGGAGGAGAGACAGAGAAGCCTCGAGGATACCAGATGTCCACCAG
  ACTAAAGATAGTGACAATCCACCAAGAACCCTTCGTGTATGTCAAGCCCACAATGAGTGATGGCACATGC
  AAAGAGGAGTTCACAGTCAATGGTGACCCTGTCAAGAAGGTGATCTGTACGGGGCCTAATGACACATCCC
  CAGGAAGCCCACGTCACACAGTGCCCCAGTGCTGTTATGGCTTCTGCGTTGACCTGCTCATCAAGCTGGC
  ACGGACCATGAATTTTACCTACGAGGTGCACCTTGTGGCAGATGGCAAGTTTGGCACACAGGAGCGGGTA
  AACAACAGCAACAAAAAGGAGTGGAACGGAATGATGGGAGAGCTGCTCAGTGGTCAAGCAGACATGATCG
  TGGCTCCACTGACCATTAACAATGAGCGTGCGCAGTACATAGAGTTCTCCAAGCCCTTCAAGTACCAGGG
  CCTGACCATTCTGGTCAAGAAGGAGATCCCTCGGAGCACACTGGACTCATTCATGCAGCCCTTTCAGAGC
  ACACTGTGGCTGCTGGTGGGGCTGTCAGTTCATGTGGTGGCCGTGATGCTGTACCTGCTGGACCGCTTCA
  GTCCCTTTGGCCGATTTAAGGTGAACAGCGAGGAGGAGGAGGAGGATGCACTGACCCTGTCCTCTGCCAT
  GTGGTTTTCCTGGGGCGTCCTGCTCAACTCTGGCATTGGGGAAGGTGCCCCCCGGAGTTTCTCTGCTCGT
  ATCCTAGGCATGGTGTGGGCTGGTTTTGCCATGATCATCGTGGCTTCCTACACTGCCAACCTGGCAGCCT
  TCCTGGTGCTGGATAGGCCTGAGGAGCGCATCACAGGCATCAATGACCCCAGGCTCAGAAACCCCTCAGA
  CAAGTTCATCTATGCAACTGTAAAACAGAGCTCTGTGGATATCTACTTCCGGAGGCAGGTGGAGTTGAGC
  ACCATGTACCGGCACATGGAGAAGCACAATTATGAGAGTGCAGCTGAGGCCATCCAGGCTGTGCGGGACA
  ACAAGCTCCATGCCTTCATCTGGGACTCAGCTGTGCTGGAGTTTGAGGCTTCACAGAAGTGCGATCTGGT
  GACCACGGGTGAGCTGTTCTTCCGCTCCGGCTTTGGCATCGGCATGCGCAAGGACAGCCCCTGGAAGCAA
  AATGTGTCCCTGTCCATACTCAAGTCCCATGAGAATGGCTTCATGGAAGACCTGGATAAGACATGGGTTC
  GGTATCAAGAATGTGACTCCCGCAGCAATGCCCCTGCCACCCTCACTTTTGAGAACATGGCAGGGGTCTT
  CATGCTGGTGGCTGGAGGCATCGTAGCTGGGATCTTCCTCATTTTCATCGAGATCGCCTACAAGCGACAC
  AAGGATGCCCGTAGGAAGCAGATGCAGCTGGCTTTTGCAGCCGTGAACGTGTGGAGGAAGAACCTGCAGG
  ATAGAAAGAGTGGTAGAGCAGAGCCCGACCCTAAAAAGAAAGCCACATTTAGGGCTATCACCTCCACCCT
  GGCCTCCAGCTTCAAGAGACGTAGGTCCTCCAAAGACACGAGCACCGGGGGTGGACGCGGCGCTTTGCAA
  AACCAAAAAGACACAGTGCTGCCGCGACGCGCTATTGAGAGGGAGGAGGGCCAGCTGCAGCTGTGTTCCC
  GTCATAGGGAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008169
• Insert Size: 2817 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008169.2, NP_032195.1
• RefSeq Size: 4326 bp
• RefSeq ORF: 2817 bp
• Locus ID: 14810
• UniProt ID: P35438
• Cytogenetics: 2 17.14 cM
• Gene Summary: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1532151, PubMed:8060614, PubMed:12008020). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:12008020).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).