Sytl2 (NM_001040085) Mouse Untagged Clone

CAT#: MC222695

Sytl2 (untagged) - Mouse synaptotagmin-like 2 (Sytl2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sytl2
• Synonyms: AI266830; mKIAA1597; Slp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222695 representing NM_001040085
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGACTTAAGTTTCCTGACAGAGGAGGAGCAAGACGCCATCTTAAAGGTTCTGCAGAGGGATGCTG
  CCCTGAAGAGGGCTGAAGAGGAGAGAGTCAGACATCTGCCTGAAAAAATTAAGGATGACCAACAACTAAA
  GAATATGAGTGGCCAATGGTTTTATGAAGCTAAGGCAAAAAGACACAGGGACAAAATCCACGGTGCTGAC
  ATCATCAGAGCATCCATGAGAAGGAAGAAGCTCCCAGCGGCAGCTGAGCAGAATAAGGACACAGCAATGA
  GGGCAAAGGAGAGCTGGGTGAATAATGTTAACAAAGATGCTGTCCTTCCTCCAGAGATAGCTGTTGTGGA
  AGAGCCAGAAGATGACACAGATCCCGCAGGCCCAAGTTCCAGTTTGGTGGATCCAGCTTCCAGTGTGATT
  GATATGTCCCAGGAAAGTACGAGGACTCCAGCTGTGTCTCTGCCCAAGCAAAGGAAGAATCCATTCAACA
  GCCCCAAGCTGCCGGAAGATCACTCCTTGCAACAAACCAAACCCGAGCAGTCAAAAACTGGAAAAGCTGG
  TTTATTTCAGATTTCAAAAGAGGGTGAGTTGTCAGAATCAAAAGAAAAGTCATCTATCCCAGATATGCCA
  CGTCAGCAGTTAGAGAAACCAAAGCAAACTGTATCCACAGAGCCTGAAAATGCGTCTCATACCAAAGCTC
  CAATCCCCAAAGCCAGAAAACTGATCTACAAATCAAATGACTTAGAGAAAGATGATAACCAGTCTTTTCC
  CAGACAACGGAGGGACTCCCTGAATGCGAGAGGGGCTCCAAGAGGGATCCTAAAGCGCAACTCCAGTTCC
  AGCAGCACGGACTCAGAGACCCTCCGTTTAAATTACAACCTTGATCCGAAAAGCAAAATCCTATCACCTG
  GCCTAACCATCCACGAGAGAATCTCTGAGAAGGAATTTTCTTTAGAAGACGACTCTTCCACAAGCTCACT
  AGAGCCGTTAAAGCACGTGAGATTCTCTGCCGTGAAGAATGAGCTTCCACAAAGTCCTAGACCCGTCCTT
  GGCCAGGAAGTGGGAGAATTTACTGTTTTAGAATCTGACCAGTTGCAAAATGGAACTGAAGATGCAGGGG
  ACATAGAGGAGTTTCAGAATCACCCAGAGCTCTCCCACAAAACACCTTTGTCTCATTATCAGTTAGTGTC
  AAGCCCAAGCGATTCAGGAAGGGAAAGAGAACAGCTGATGTCTTCTGGGTCTGCTCCAAGAGATGAGATC
  CCTTGTCATTCAGACATTCTACCCACAGGACCTCAGTGTGTTGAGAGTTCATCCGTCATCAATGGGCAAC
  AAGAGAAATCATCACATTTTACAAAGCTTCCATCAGAATTGTCCAAAAGCCCTTCCGATGAACTGACTCA
  GTGTGGTGAGCCTGAGCCATCACAGACAGCAGACCACAGTTTTAGAGACCATCGGCAAGGTTCAGAAGAA
  GAGCACAGCCCTGTTTTGAAAACTCTGGAAAGAAGAGCTGCTAGGAAATTGCCTTCCAAAAGTCTAGAAG
  ACATTCCATCAGATTCATCAAATCAAGCAAAAGTAGATAATCTGCCTGAAGAATTAGTGCGTAGTGCTGA
  AGATGTTTCCACAGTGCCTTCACTGCCTGATAATCAGTTTTCCCACCCTGACAAACTCAAAAGGATGAGC
  AAGTCCGTGCCAGCATTTCTTCAAGATGAGAGCGATGACAGAGAAACAGACACAGCATCAGAGAGCAGCT
  ACCAGCTCAGGAGATACAAGAAGAGCCCCAGCTCATTAACCAATCTTAGCAGCTCCTCTGGCATGACGTC
  CTTGTCCTCTGCGAGTGGCAGTGTGATGAGCGTTTACAGTGGAGACTTTGGCAACCTAGAAGTGAAAGGA
  AGCGTGCAGTTTGCACTCGACTACGTGGAGTCCCTGAAAGAGCTGCATGTGTTTGTGGCCCAGTGTAAGG
  ATTTAGCAGCAGCAGATGTTAAGAAACAGCGCTCAGATCCGTATGTAAAGACCTATCTGCTACCAGACAA
  AGGCAAAATGGGCAAGAAGAAGACACTCGTAGTGAAGAAGACCTTGAATCCTGTATACAACGAGATATTG
  CGGTATAAAATTGAAAGGCAATTCTTAAAGACGCAGAAGTTGAACCTGTCCGTTTGGCATCGGGATACAT
  TTAAGCGCAACAGCTTTCTGGGGGAGGTGGAGCTCGACCTGGAAACGTGGGATTGGGACAGCAAACAGAA
  CAAACAGCTGAAGTGGTACCCACTGAAGAGGAAGACAGCACCAGTTGCCCTCGAGACAGAAAACAGAGGT
  GAAATGAAACTAGCTCTCCAGTATGTTCCGGAACCAAGCCCTGGCAAAAAGCTTCCTACAACTGGAGAAG
  TCCACATCTGGGTGAAGGAATGCCTTGACCTCCCACTGTTGAGGGGCAGCCACCTAAATTCTTTTGTTAA
  ATGTACCATCCTTCCAGATACCAGTAGAAAAAGTCGCCAGAAGACAAGAGCTGTAGGGAAAACCACCAAC
  CCCGTCTTCAACCATACCATGGTGTATGATGGGTTCAGGCCTGAAGATCTGATGGAAGCCTGTGTAGAAC
  TCACAGTCTGGGACCATTATAAACTAACCAACCAGTTTCTGGGAGGTCTCCGGATCGGCTTTGGAACAGG
  AAAAAGCTACGGGACTGAAGTGGATTGGATGGATTCTACTTCTGAGGAAGTTGCTCTCTGGGAGAAGATG
  GTAAACTCTCCCAACACTTGGGTTGAAGCGACGCTGCCCCTCCGGATGCTTCTGATTGCCAAGCTTTCCA
  AGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040085
• Insert Size: 2805 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040085.2, NP_001035174.1
• RefSeq Size: 4731 bp
• RefSeq ORF: 2805 bp
• Locus ID: 83671
• UniProt ID: Q99N50
• Cytogenetics: 7 E1
• Gene Summary: Isoform 11 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. Required for cytotoxic granule docking at the immunologic synapse. Isoform 1 may play a role in melanosome transport and vesicle trafficking. It controls melanosome distribution in the cell periphery and regulates melanocyte morphology. Isoform 1 acts as a positive mediator of mucus secretion by the surface mucus cells of the stomach. Mediates basal mucus secretion by gastric surface cells by promoting the proper granule biognesis and docking of mucus granules with the apical plasma membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 6, resulting in an isoform (2, also known as Slp2-a delta 2S-II) that is shorter than isoform 6.