Erap1 (NM_030711) Mouse Untagged Clone

CAT#: MC222694

Erap1 (untagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erap1
• Synonyms: Arts1; ERAAP; PILSA; PILSAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222694 representing NM_030711
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCTCTTCTTCCCCTAGTATTGACATTTTTATCTGTGTCATCTCCTTCTTGGTGTCAGAACAGTG
  ATATAGAATCTCTAAAAGCTAGTAATGGAGACTCATTCCCTTGGAATAATATGCGACTTCCTGAGTATAT
  GACCCCGATTCATTATGATCTCATGATCCATGCAAACCTCAGCACTCTGACTTTCTGGGGAAAAACAGAA
  GTAGAAATCATAGCTAGCCGGCCCACCAGCACCATTATTATGCATAGTCACCACCTGCAAATATCTAAGG
  CCACCCTCAGAAGAGGAGCTGGAGAGATGCTGTCTGAAGAACCACTGAAGGTCCTGGAATATCCTGCTCA
  TGAGCAAGTTGCACTGCTGGCTGCCCAGCCGCTTCTTGCTGGGTCCCTGTACACAGTTATCATCGACTAT
  GCTGCCAACCTGTCTGAGAGTTTCCATGGATTCTATAAGAGCACCTACAGAACACAGGAAGGTGAAATGA
  GAATACTTGCAGCAACACAGTTTGAACCCACAGCTGCTAGAATGGCTTTTCCCTGCTTTGATGAACCTGC
  CCTCAAGGCAAGTTTTTCCATCAAGATAAAGAGGGATCCAAGGCACCTGGCCATCTCCAACATGCCCCTG
  GTGAAATCTGTGAATGTTGCTGAAGGACTCATAGAAGACCATTTTGACATCACTGTGAAGATGAGTACCT
  ACCTAGTGGCCTTCATCATTTCTGATTTTAAGTCTGTGAGCAAGATGACTAAGAGTGGAGTCAAGGTTTC
  TGTGTATGCTGTGCCAGACAAGATAAATCAAGCCGATTATGCTCTGGATGCTGCAGTGACTCTTCTAGAG
  TTTTATGAGGATTATTTCAACATTCCATATCCTTTACCCAAGCAAGATCTTGCTGCAATCCCAGACTTTC
  AGTCTGGTGCTATGGAAAACTGGGGACTGACCACATACAGAGAGTCCTCTCTGTTATACGATAAAGAAAA
  GTCTTCTGCATCTAGTAAGCTTGGCATCACAATGATTGTGTCCCATGAACTGGCTCACCAGTGGTTCGGG
  AACCTGGTCACCATGGAATGGTGGAATGACCTTTGGCTCAATGAAGGATTTGCCAAATTTATGGAGTTTG
  TGTCTGTCACTGTGACCCATCCTGAACTAAAAGTTGAAGACTATTTCTTTGGCAAGTGTTTTAATGCAAT
  GGAAGTTGATGCATTAAACTCCTCTCACCCTGTATCCACACCTGTGGAGAATCCTGCGCAGATTCGGGAG
  ATGTTTGATGATGTTTCTTATGAAAAGGGAGCTTGTATTCTGAATATGCTAAGAGATTATCTGAGTGCAG
  ATACATTTAAAAGAGGAATTGTACAGTATCTCCAAAAGTATAGTTATAAAAACACAAAAAACGAGGACCT
  GTGGAATAGCATGATGCATATTTGCCCTACAGATGGCACACAAACAATGGATGGCTTCTGCTCTAGAAGC
  CAACACTCATCATCAACCTCACACTGGCGTCAGGAGGTTGTAGATGTAAAGACCATGATGAACACATGGA
  CACTGCAGAAGGGCTTTCCTCTGATAACCATCACTGTGAGTGGGCGGAATGTGCACATGAAGCAAGAACA
  CTACATGAAGGGTTCAGAACGCTTCCCAGAGACTGGGTATTTGTGGCATGTTCCACTGACATTCATTACC
  AGCAAATCAGACTCAGTCCAGAGATTTTTGCTGAAGACAAAGACAGATGTGCTCATCCTCCCAGAAGCAG
  TGCAGTGGATCAAATTTAACGTGGGAATGAATGGCTATTACATTGTGCATTACGCGGATGATGGATGGGC
  TTCTCTGAGTGGCCTTTTAAAAGAAGCGCACACAACAATCAGCAGTAATGATCGGGCAAGTCTCATCAAC
  AATGCATTTCAGCTGGTCAGCATTGAAAAGTTATCAATAGAAAAAGCCCTGGATTTAACCCTGTACTTGA
  AAAATGAAACTGAAATTATGCCCATATTCCAAGCTTTGAATGAACTCATACCTATGTATAAGTTAATGGA
  GAAAAGAGATATGATTGAGGTGGAAACTCAATTTAAGGACTTCCTTCTCAAGTTGCTGAAGGACCTTATT
  GATAAGCAGACTTGGACAGATGAAGGCTCTGTCTCAGAGAGGATGCTCAGGAGCCAGCTCCTCCTCCTCG
  CATGTGTGCGCAATTATCAGCCCTGTGTGCAGAGGGCAGAGCGCTATTTCAGAGAGTGGAAGTCATCTAA
  TGGAAACATGAGCATCCCTATTGATGTGACCTTGGCTGTGTTTGCTGTCGGGGCCCAAAACACAGAAGGC
  TGGGATTTTCTTTATAGTAAATATCAGTCTTCTTTGTCTAGTACTGAGAAAAGCCAAATTGAATTTTCCC
  TCTGTACAAGCAAAGATCCAGAAAAACTTCAGTGGCTTCTAGATCAAAGTTTTAAGGGAGAGATAATAAA
  AACTCAGGAATTTCCACATATTCTCACACTCATTGGCAGAAACCCAGTGGGTTATCCATTGGCCTGGAAG
  TTTCTGAGGGAAAATTGGAATAAGCTTGTACAGAAATTTGAACTTGGCTCATCGTCCATAGCTCACATGG
  TAATGGGAACAACAGACCAGTTTTCCACCAGAGCACGTCTTGAAGAGGTAAAAGGATTCTTCAGTTCCTT
  GAAAGAAAACGGTTCTCAGCTCCGTTGTGTTCAACAAACCATTGAGACCATTGAAGAAAACATACGATGG
  ATGGATAAGAATTTTGATAAAATAAGACTGTGGCTGCAAAAAGAAAAGCCAGAGTTGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030711
• Insert Size: 2793 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030711.4, NP_109636.1
• RefSeq Size: 4014 bp
• RefSeq ORF: 2793 bp
• Locus ID: 80898
• UniProt ID: Q9EQH2
• Cytogenetics: 13 C1
• Gene Summary: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (By similarity).[UniProtKB/Swiss-Prot Function]