Adamts5 (NM_011782) Mouse Untagged Clone

CAT#: MC222688

Adamts5 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts5
• Synonyms: 9530092O11Rik; ADAM-; ADAM-TS5; ADAMTS1; ADAMTS11; ADMP-2; AI481094; ASMP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222688 representing NM_011782
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTCGAGTGGGCGCCCTTGTTGCTGCTACTGCTGCTGCTGAGCGCGTCCTGCCTGTCCCTGGCCG
  CTGACAGCCCCGCCGCGGCACCTGCCCAGGATAAAACCAGGCAGCCTCAGGCTGCAGCAGCGGCCGCCGA
  GCCGGACCAGCCGCAGGGGGAGGAAACACGGGAGCGAGGCCATTTACAACCCTTGGCCGGGCAGCGCAGG
  AGCGGCGGGCTGGTCCAGAATATAGACCAACTCTACTCTGGCGGTGGCAAAGTGGGCTACCTTGTCTACG
  CGGGCGGCCGGAGGTTCCTGCTGGACCTGGAGAGAGATGACACAGTGGGTGCTGCTGGTAGCATCGTTAC
  TGCAGGAGGAGGGCTGAGCGCATCCTCTGGCCACCGGGGTCACTGTTTCTACAGAGGCACCGTGGACGGC
  AGCCCTCGATCCCTAGCTGTCTTTGACCTCTGCGGGGGTCTCGATGGCTTCTTTGCAGTCAAGCATGCGC
  GCTACACTCTAAAGCCACTCCTGCGTGGGTCCTGGGCAGAGTATGAACGAATTTATGGGGATGGATCTTC
  CCGCATCCTGCATGTCTACAACCGCGAGGGCTTTAGCTTCGAGGCCCTGCCGCCACGCGCCAGTTGCGAG
  ACTCCTGCATCCCCATCTGGGCCCCAAGAGAGCCCCTCGGTGCACAGTAGATCTAGGAGACGCTCAGCGC
  TGGCCCCGCAGCTGCTGGACCACTCAGCTTTCTCGCCATCTGGGAACGCGGGACCTCAGACTTGGTGGAG
  GCGTAGGCGCCGTTCCATCTCCAGGGCCCGCCAGGTGGAGCTCCTCTTGGTGGCTGACTCGTCCATGGCC
  AGGATGTATGGGCGGGGCCTGCAGCATTACCTGCTGACCCTGGCCTCCATCGCCAACAGGCTGTACAGTC
  ATGCAAGCATTGAGAACCACATCCGCCTGGCGGTGGTGAAGGTGGTGGTGCTGACGGACAAGGACACGAG
  TCTGGAGGTGAGCAAGAATGCGGCCACGACCCTCAAGAACTTTTGCAAATGGCAGCACCAACATAACCAG
  CTAGGGGATGATCACGAAGAGCACTACGATGCAGCCATCCTGTTCACCCGAGAGGATTTATGTGGGCATC
  ATTCATGTGACACCCTGGGAATGGCAGACGTTGGGACCATATGTTCTCCGGAGCGCAGCTGTGCAGTGAT
  TGAAGATGATGGCCTCCATGCAGCCTTCACTGTGGCTCATGAAATTGGGCATCTACTTGGCCTTTCTCAT
  GACGATTCCAAATTCTGTGAAGAGAACTTCGGTACTACAGAAGACAAGCGTTTAATGTCTTCAATCCTTA
  CCAGCATCGATGCATCCAAGCCCTGGTCCAAATGCACGTCAGCCACCATCACAGAATTCCTGGATGATGG
  TCATGGTAATTGTTTGCTAGACCTACCACGGAAGCAGATTTTGGGTCCCGAGGAACTCCCAGGACAGACC
  TACGATGCCACCCAGCAGTGCAACTTGACATTTGGGCCTGAGTACTCGGTGTGCCCTGGCATGGATGTCT
  GTGCGCGGCTGTGGTGTGCTGTGGTGCGCCAAGGCCAAATGGTGTGTCTGACCAAGAAGCTGCCGGCTGT
  GGAGGGCACTCCCTGTGGGAAGGGAAGAGTCTGCCTTCAAGGCAAATGTGTGGACAAAACTAAGAAAAAA
  TATTACTCGACATCAAGCCATGGAAATTGGGGGTCCTGGGGCCCCTGGGGTCAGTGTTCTCGCTCATGCG
  GGGGAGGAGTGCAGTTTGCCTACCGCCATTGTAATAACCCTGCACCTCGAAACAGTGGCCGCTACTGCAC
  AGGGAAGAGGGCCATATACCGTTCCTGCAGTGTTACACCCTGCCCACCCAATGGTAAATCTTTTCGCCAT
  GAGCAGTGTGAAGCCAAAAATGGCTATCAGTCTGATGCAAAAGGAGTCAAAACATTTGTAGAATGGGTTC
  CCAAATATGCAGGTGTCCTGCCGGCAGATGTGTGCAAGCTTACCTGCAGAGCTAAGGGCACAGGCTACTA
  TGTGGTCTTTTCTCCAAAGGTTACGGATGGGACTGAATGCAGGCCGTACAGCAACTCTGTGTGTGTCCGA
  GGACGGTGTGTGAGAACTGGATGTGACGGCATTATTGGCTCAAAGCTACAATATGACAAGTGTGGAGTGT
  GCGGAGGGGATAACTCCAGTTGTACAAAGATTATCGGAACCTTCAATAAAAAAAGCAAGGGTTATACTGA
  CGTTGTGAGGATCCCTGAAGGAGCAACCCACATAAAAGTCCGACAGTTCAAAGCCAAAGACCAGACTAGA
  TTCACTGCCTACTTAGCCCTGAAGAAGAAAACTGGCGAGTACCTTATCAATGGCAAGTACATGATTTCCA
  CTTCAGAGACCATCATCGACATCAATGGTACCGTCATGAACTACAGTGGATGGAGCCACAGAGATGATTT
  TTTACATGGGATGGGCTATTCAGCCACAAAAGAAATCCTGATCGTGCAGATCCTTGCCACAGACCCAACT
  AAAGCGCTAGACGTCCGTTACAGCTTTTTTGTTCCCAAGAAGACCACTCAAAAAGTAAACTCTGTCATCA
  GCCATGGCAGCAACAAGGTGGGACCACACTCTACACAGCTGCAGTGGGTGACAGGTCCATGGCTGGCCTG
  CTCCAGGACCTGTGACACAGGCTGGCACACTAGGACCGTGCAGTGCCAGGATGGAAACAGGAAATTAGCT
  AAAGGATGCCTTCTCTCTCAGAGGCCTTCTGCATTTAAGCAATGTCTGCTGAAGAAATGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011782
• Insert Size: 2793 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011782.2, NP_035912.2
• RefSeq Size: 8104 bp
• RefSeq ORF: 2793 bp
• Locus ID: 23794
• UniProt ID: Q9R001
• Cytogenetics: 16 48.34 cM
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. Mice lacking the encoded protein are protected from surgery-induced osteoarthritis and antigen-induced arthritis. [provided by RefSeq, May 2016]