Tmtc3 (NM_001110013) Mouse Untagged Clone

CAT#: MC222668

Tmtc3 (untagged) - Mouse transmembrane and tetratricopeptide repeat containing 3 (Tmtc3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmtc3
• Synonyms: 9130014E20Rik; B130008E12Rik; mSmile
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222668 representing NM_001110013
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGAAGGGAAGATGGCTGATATTAACTTCAAAGAAGTGACTTTAATAGTCAGCGTGGTTGCCGCCT
  GCTACTGGAACAGCCTGTTCTGTGGGTTTGTTTTTGATGATGTTTCAGCAATATTGGATAATAAAGACCT
  GCATCCATCTACACCTTTAAAAACTTTATTTCAGAATGACTTCTGGGGAACTCCTATGTCTGAGGAGAGA
  AGCCACAAGTCATACAGGCCCTTAACGGTGTTGACATTTCGCTTAAATTATCTCCTCAGTGAGCTGAAAC
  CCATGTCTTACCACCTCCTGAACACAGTGTTCCACGCTGTCGTCAGCGTGATCTTCCTTAAGGTCTGCAG
  ACTCTTTCTGGATAAGAGGAGCAGCATGATTGCGGCTCTGCTCTTCGCAGTGCACCCAATCCATACCGAG
  GCAGTCACAGGTGTTGTAGGAAGAGCGGAACTCTTATCATCTGTCTTTTTCCTAGCTGCCTTTCTCTCGT
  ATACTAAGTCAAAAGGACCAGATAATTCCATAGTGTGGACCCCGATTGTATTAACCGTGTTTTTAGTGGC
  TGTTGCAACATTGTGTAAGGAACAAGGAATAACGGTTGTCGGAATTTGTTGTGTATATGAAGTATTTGTG
  GCCCAAGGGTACACCTTGCCGATGTTATGCACCGTTGCCGGGCAGTTTCTGCGCGGGAAGGGCAGCATTC
  CGTTATCTATGCTGCAGACACTGGTGAAGCTCATTGTCCTGATGCTCAGTACCTTGCTACTCGTTGTGGT
  CAGAGTCCAAGTTATTCAGTCACAACTTCCAGTGTTTACCAGGTTTGATAATCCAGCTGCTGTAAGCCCA
  ACCCCGACACGACAGCTGACTTTTAACTATCTCCTTCCTGTGAATGCCTGGCTTCTGCTGAATCCTTCGG
  AGCTCTGCTGTGATTGGACCATGGGGACAATACCACTGATAGAATCATTTCTAGATGTTCGAAATCTCGC
  CACTTTTGCTTTCTTTTGCTTTCTGGGGGCTTTGGGAATATTCAGTCTCAGATACCCTGGTGACTCCTCA
  AAGACTGTCCTAATGGCGCTTTGTTTAATGGCGTTACCATTTATTCCCGCATCAAACCTGTTCTTTCCTG
  TTGGATTTGTGGTTGCTGAGCGAGTACTATATGTTCCTAGCATGGGGTTCTGTATTTTAGTAGCCCATGG
  ATGGCAAAAAATTTCAAACAAAAGTGTGCTGAAAAAGCTCTCGTGGGTTTGTCTGTCCATGGTGATACTA
  ACCCATGCCTTGAAAACACTTCATAGAAATTGGGACTGGGAGTCAGAATATACATTGTTTATGTCAGCCC
  TAAAGGTGAATAAAAACAATGCCAAATTATGGAATAATGTGGGTCATGCTCTGGAGAATGAGAAGAACTT
  TGAGAAAGCTTTGAAATACTTCTTGCAGGCTACCCATGTTCAGCCAGATGACATCGGTGCCCACATGAAT
  GTAGGAAGAACTTATAAAAACTTAAACAGAACTAGAGAAGCTGAAGCGTCTTACATGCTGGCTAAATCAC
  TGATGCCTCAGATTATCCCTGGTAAAAAATATGCAGCCAGAATTGCCCCTAATCACCTAAACGTTTATAT
  CAATCTGGCCAACCTTATTCGAGCAAATGAGTCCCGCCTGGAGGAAGCGGACCAGCTGTACCGACAGGCC
  ATCAGCATGAGGCCAGACTTCAAACAGGCTTACATTAGCAGGGGAGAGTTGCTTTTAAAAATGAATAAGC
  CTCTCAAAGCAAAGGAAGCATATCTTAAAGCACTAGAGCTGGACAGAAATAATGCAGATCTCTGGTACAA
  CTTGGCAATTGTTTATATTGAACTTAAAGAACCAAATGAAGCTCTGAAGAACTTTAATCGAGCTTTGGAA
  CTGAACCCTAAACATAAGCTAGCACTGTTCAATTCTGCTATTTTAATGCAGGAATCAGGTGAAGTTAAAC
  TCAGACCTGAAGCTAGAAAACGGCTTCTAAATTATGTAAATGAAGAGCCACAGGATGCTAATGGCTATTT
  CAATTTGGGGATGCTCGCCATGGATGACAAAAAGGATTCAGAAGCTGAGTCTTGGATGAAGAAAGCTATC
  AAATTACAACCTGACTTCAGAAGCGCTTTGTTTAACCTGGCACTCCTGTATTCGCAGACTGCTAAGGAGT
  TAAAGGCATTGCCAATTTTAGAAGAGCTGCTTAAATACTATCCTGACCATACCAAGGGTCTCATTTTAAA
  AGGAGACATTCTGATGAACCAAAAGAAGGATATACCTGGAGCTAAAAAGTGTTTTGAAAAGATCCTGGAA
  ATGGATCCAAGCAATGTGCAGGGGAAGCACAACCTGTGTGTTGTGTACTTTGAAGAGAAGGAGTTACTAA
  AAGCTGAGAGGTGTCTTGTGGAAACATTGGCATTAGCACCTCATGAGGAGTATATTCAACGCCATTTGAG
  TATAGTCAGGGATAGAATCTCCTCTTCTGGTATTGTGGAGCAGCCACTGGCCCCAGCTGATAAGACTCCA
  GGTACAGAGGAGAGAGAGGAAATCCCTTCTGAGGATGTAAAAGAAATCAGCAGTGAATCCAGACCACCAC
  AAATCTTAAAAACAAACAATAACAGAAACTCTAAATCCAACAAGCAATCAACAGAAAATGCAGACCAAGA
  TGCCCCCCATAAAACAACAAAAGACATCAAAGAAATTGAGAAGAAAAGAGTTGCTGCTTTGAAAAGGCTA
  GAAGAGATTGAACGTATTTTAAATGGAGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110013
• Insert Size: 2763 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110013.1, NP_001103483.1
• RefSeq Size: 5990 bp
• RefSeq ORF: 2763 bp
• Locus ID: 237500
• UniProt ID: Q8BRH0
• Cytogenetics: 10 D1
• Gene Summary: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3. Involved in the positive regulation of proteasomal protein degradation in the endoplasmic reticulum (ER), and the control of ER stress response.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.