Mcf2 (NM_133197) Mouse Untagged Clone

CAT#: MC222652

Mcf2 (untagged) - Mouse mcf.2 transforming sequence (Mcf2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcf2
• Synonyms: B230117G22Rik; Dbl; Mcf-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222652 representing NM_133197
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAGCAAATCCCCCGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCCTCCTTCCCTGGGAGCT
  TACACTTAGTCCTGGTTTTGCGTCCTACCAGTTTTCTTCAACGGACTTTCACGGACATTGGATTTCGATT
  TAGCCAGGAGGATTTTATGCTGAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATAC
  ATTGATGACAAGCAGTTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCT
  TCAGAAATGCCATAGAGAAGTTTGCTGTCACAGTGAAAGAAATGGCTCAGATGTTGCAATCCTTTGGAAC
  AGAATTGGCTGAAACAGAGCTACCACAGGATATCCTTTCAATAGAAGAGATTCTGGCAGGTCGAGCCGAA
  AGGTATCACCTGTTGAAGAATGACTTAACTGCTGTAACCAAAGAAGGAAAAGTTCTGCTCATGAACCTGC
  AAGTGCCTGCCACTGAAGAAACTGTCAGTTCAAGCCTTGAATGTACTCAGCACATAAATGGCGACTGGCA
  AACTATTAAAAAGTTGCTGGCTCAGGTGCATGATATGGAAACTGCTTTTGATGGATTTTGGGAAAAACAT
  CAGCTGAAAATGGAGCAGTATCTGCAACTGTGGAAGTTTGAACAAGACTTTCAAGAGGCTGTGACTCAAG
  TTGAATTTTTATTAAGTCAACAAAGAGAACTGGGTGATATAACAGGGAACTTAGCTCAAGTAAAACAAAG
  ACTTAAAAAACTAGAGATCTTAGATGATAAATCTCAGGAACTGTTAACCAAGGCCCGGATTGTGATACTA
  CGTGGCCACAAGCTGGCATCGAATCACCACTATGCACTTGATTTAATTTGCCAGCGATGCAATGAACTAC
  GCTACCTTTCTGATATTTTAGTTAATGAGATCAGAACAAAGCGGGTCCAACTGAGCAGGACTTTCAAAGT
  GCATAGACTCTTACAGCAGGCACGTCAGTGCTGTGATCAAGGGGAATGCCTCCTGGCTAGCCAAGGAATG
  GATAAGTTTCAGACTAAAGAAGATGCTCAGAAAGCCCTTCAGGATGTGGACAATTTCCTTCAAATGGCCA
  TGCCTTTCATCAATTATGATATCGAAAGCCTGCAATATGAATTTGATGTACTCCTATCTCCTGAACTCAA
  GGCTCAAATGCAGAATATACAACTAAAGCTTGAAAATATTCGAAGTGCATTTCAGAACCAACAGGCTGGT
  TGTAAGAGCCTAAAAGAAGTACCCGAGGGGGCGTTCCAGAACTTGGTTCCTGCATCTGAAAATGTGATGC
  GGTCCAGAATGATATTCTTTTCACCGAAACATGTGAAAAAGTCTTGGAGACAGATTCACGCTCAGAGCAA
  TGTGAAAGTTGAAGCCGTTGAGGATAGCCAAGAGAAGAATTCTGATCAGTCTCCCAAATTGGACAATAGC
  TTGGATATTTTAAAGAACCATGTCCTAAATGAGCTGATACAGACAGAGAGAGCATATGTTCGAGAGCTGT
  TTACTGTTTTGTTGGGCTACAGATCTGAGATGGACAATCCACAGATGTTTGATCTTATGCCACCTCTCCT
  GAGAAATAAAAAGGATGTTCTCTTTGGAAATATGGCAGAAATATACGAATTCCATAACAACATTTTCATG
  AGCAGACTGGAAGATTGCTCTGATGCTCCAGAAAGAGTGGGACCTTGCTTCCTGGAAAGGAAGGATGATT
  TTCAGATGTATGCCAAATATTGTCAGAATAAACCCAGGTCAGAGTTAATTTGGAGGAAGTATTCTGAATG
  TGCCTTTTTCCAGGAATGTCAACGAAAATTGAAACATCGACTTGGTCTGGATTCCTATTTGCTCAAACCT
  GTTCAACGAATCACTAAGTATCAGTTGCTTTTAAAGGAGCTTTTAAAGTATAGCAAAGAAGGTGAAGGAA
  CTACTAAATTAAAGGAGGCACTTGACTCAATGCTGGAGCTGCTGAAGTCAGTGAATGACTCCATGCATCA
  GACTGCCATCAATGGCTATGTTGGAAATATAAATGAGCTGGGCAAGATGGTTCTTCAAGGTTCCTTCAAT
  GTTTGGCTAGGACACCGGAAAGGTGCTACAAAAATGAAGGATTTTGCACGTTTCAAACCCATGCAGAGAC
  ATCTTTTCTTATATGAAAAAGCTGTTATGTTTTGTAAGAGGCGTTTCGAATCTGGAGAAGGTGCTGATAG
  ATACCCATCATACAGTTTTAAGCACTGCCTAAAAATGGAAGATGTTGGCATCACAGAACATGTGAAAGGT
  GATAACCGCAAATTTGAAATACGGTATAGTGAGAAGGAAGAAATTTACATTGTTCAGGCTCCAAATGTTG
  ATGTGAAGATGTTATGGCTAAAAGAAATAAGAAAAATTTTGGTGCAGCAGAAGGAACTTATGACTGCTAA
  AACCCAGCAGGATCAGGCACTAGACCAGGACCAACTTTTCCCCCAGCAGCAGAATGCCGAGCTATGCAAA
  AGCTCACCTTTTTGTGTGTGTGAGGAAACTCTTTTCAACGCCACAGAAGCAGGAGCAGAAGTGGAGCAAG
  CTGGTGCCCTGATAAAGGTCAGGGAGGCAGCTGTGCTAGCTCAAGCAGAGGCAAGTTCAGCAGCTTGGAA
  TGGGATGTTACCATCTGCAGAAGGCGCTGCTGCAATTGCTGAACATTCATACAATTATTTCTCCTCTAAC
  AATGACCATGGAGAAGATAGGACACAAATGAGACACATGTCGGAGGTGACATTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133197
• Insert Size: 2787 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133197.4, NP_573460.2
• RefSeq Size: 3671 bp
• RefSeq ORF: 2787 bp
• Locus ID: 109904
• Cytogenetics: X 33.5 cM