Als2 (NM_146109) Mouse Untagged Clone

CAT#: MC222644

Als2 (untagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) homolog (human) (Als2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Als2
• Synonyms: 3222402C23Rik; 9430073A21Rik; Als2cr6; Alsin; mKIAA1563
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222644 representing NM_146109
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCAAAGAAGAAAAGCTCAACAGAGGCAGAAGGATCCAAAGAAAGAGGCCTAGTCCATGTCTGGC
  AGGCAGGATCCTTTTCTCTAACACCAGAGAGGTTGCCAGGCTGGGGTGGAAAGACAGTTCTTCAGGCAGC
  CCTTGGTGTGAGGCATGGAGTTCTTCTGACTGAAGATGGTGAGGTCTACAGCTTTGGGACTCTTCCCTGG
  AAAAGTGAATCAGCAGAAATTTGTCCAAGCAGCCCCCTTCTAGAAAGTGCCCTGGTTGGGCATCATGTTA
  TTACTGTGGCAACAGGGAGCTTCCACAGTGGAGCAGTGACAGAGAGCGGGGTGGTGTACATGTGGGGAGA
  GAATGCTGCCGGGCAGTGTGCGGTAGCTAACCAGCAGTATGTGCCGGAGCCGAGTCCTGTCAGCATTTCT
  GACTCGGAGACCAGCCCGTCATTAGCAGTTAGGATTCTGCAATTGGCATGTGGCGAGGAACACACACTGG
  CATTGTCACTCAGCAGAGAGATCTGGGCATGGGGCACCGGCTGTCAGCTGGGCCTCATCACCACCACTTT
  CCCAGTGACAAAGCCACAGAAGGTGGAACACCTTGCTGGACGCGTGGTGCTCCAGGTGGCCTGCGGTGCA
  TTCCACAGCCTTGCACTTGTGCAGTGCCTCCCTCCTCAGGATCTGAAGCCAGTCCCAGAGAGATGCAATC
  AGTGCAGCCAGCTGCTCATCACCATGACAGACAAAGAGGACCATGTGATAATATCGGACAGCCATTGCTG
  CCCTTTAGGTGTGACATTGTCCGAGTCTCAAGCAGAAAAGCATGCCAGCCCTGCTCCCAGCCCTCACCCA
  GAGGCACTGGATGAGCAGGGAGAGGTGTTTGAGAACACGGTGGTAGAAGCTGAACTGAACATGGGAAGCA
  GTCAGACCACAAGTGGCAGTGCCATTTCCACCCAGCAGAACGTCGTGGGAACAGCTGAAGTGTCTTCTGC
  CAGAACAGCTCCGTCATACCCAGACACCCATGCGGTAACTGCATACCTGCAGAAGCTGTCAGAGCATTCG
  ATGAGGGAGAACCATGAGCCTGGAGAAAAGCCACCCCAGGTCCAGCCTCTTGTAGAAGAAGCAGTTCCTG
  ATCTTCACAGTCCACCAACCACAAGCACCTCAGCCCTCAACAGCTTGGTGGTCTCCTGTGCATCTGCTGT
  TGGTGTCAGAGTGGCTGCCACCTATGAAGCTGGGGCCTTGTCTCTCAAGAAAGTTATGAACTTTTACAGC
  ACTGCCCCCTGCGAGACGGCAGCTCAGTCGGGCAGTGCCTCCACAGGCCCAGAAAGTCTGAAAGATCTCC
  GAGAAGAGCAGGTGAAACAGGAGTCACTGCAAGGGAAGAAAAGCTCAAGTCTCATGGACATCAGAGAGGA
  GGAGTTGGAGGGAGGGAGTCGAAGACTCTCCCTCCCAGGGTTGTTGTCGCAAGTTTCCCCCAGGCTCTTA
  AGGAAGGCTGCGAGGGTGAAAACTCGGACAGTGGTTCTGACTCCCACATACAGTGGAGAAGCAGATGCCC
  TTCTGCCTTCCCTGAGGACAGAGGTGTGGACCTGGGGGAAAGGCAAGGAAGGGCAGCTAGGGCACGGCGA
  CGTCCTGCCCAGGCTTCAGCCGTTGTGTGTCAAGTGTCTGGATGGTAAAGAGGTAATCCACCTGGAGGCG
  GGCGGCTCCCACTCCCTCGCACTCACTGCGAAATCTCAGGTTTACTCATGGGGCAGTAATACCTTTGGTC
  AGCTTGGGCATTCTGAGTTTCCAACAACGGTTCCTCGACTCTCAAAGGTTAGCAGTGAAAATGGAGTCTG
  GAGTGTAGCTGCAGGCCAAGATTATTCCTTGTTTTTAGTGGACACGGAAGACTTCCAGCCTGGGTTGTAT
  TACAGTGGCCGACAGGACCGTGCAGAAGGTGATACCCTGCCAGAGAATCCCAGTGGTACAAAGACTCCAG
  TACTTCTCTCCTGTAGTAAGCTTGGATACATAAGCAGAGTAACAGCAGGAAAAGATAGCTATCTAGCCTT
  GGTGGATAAGAACATCATGGGATACATCGCCAGTCTCCATGAGTTGGCTTCTACAGAAAGACGGTTTTAC
  TCAAAACTGAGCGAAATCAAATCACAGATACTTAGGCCTCTTCTCAGTTTAGAAAATTTGGGCACAGTGA
  CCACTGTCCAGCTGTTGCAGGAAGTTGCCAGCCGGTTCAGCAAGTTGTGTTACCTCATTGGGCAGCATGG
  AGCCTCACTAAGCAGCTACCTACAGGGCATGAAGGAAGCCAGCAGCCTGGTCATCATGAAGCACTCAAGT
  CTTTTCCTGGACAGCTACACAGAGTACTGCACATCAGTTTCAAATTTCCTGGTTATGGGAGGATTCCAGC
  TTCTTGCTAAGCCTGCCATTGATTTCCTAAATAAAAACCAAGAACTCTTGCAAGATTTGTCAGAAGTGAA
  TGATGAGAACACTCAGTTGATGGAAATCCTGAACATGCTGTTTTTCTTGCCAATCAGACGACTTCATAAT
  TATGCAAAAGTTTTGCTAAAGCTTGCCACTTGCTTTGAAGTGGTAGGTTTCGTGTGTGCCCCTCCAAATA
  CTCGGGAAGCCAAGAGTCAAAGTAGTTTGTTTGCATTAAGTCTCCTTAAGATGTTGGGCCCCGGGGCTGG
  TGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCCGGAGTTCAAATCCCAGCAACCA
  CATGGTGGCTCACAACCACCCGTAATGAGATCTGACGCCCTCTTCTGGAGCTTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_146109
• Insert Size: 2787 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146109.3, NP_666221.1
• RefSeq Size: 2977 bp
• RefSeq ORF: 2787 bp
• Locus ID: 74018
• UniProt ID: Q920R0
• Cytogenetics: 1 C1.3
• Gene Summary: May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding sequence compared to variant 3. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.