Ptpn4 (NM_019933) Mouse Untagged Clone

CAT#: MC222633

Ptpn4 (untagged) - Mouse protein tyrosine phosphatase, non-receptor type 4 (Ptpn4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptpn4
• Synonyms: hPTP-MEG; Ptn4; PTPMEG; TEP; TEP/mPTPMEG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222633 representing NM_019933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCACGTTTCCGATTGCCTGCTGGCAGAACCTACAATGTCCGAGCATCAGAGTTGGCCCGAGACA
  GACAGCATACAGAGGTCGTTTGCAACATTCTTCTTCTGGATAACACTGTACAGGCTTTCAGAGTTAACAA
  ACATGATCAGGGGCAAGTTCTGTTGGATATAGTCTTCAAGCATCTTGATTTGACTGAGCGAGACTATTTT
  GGTTTACAGTTGGCTGACGATTCCACAGATAACCCAAGGTGGCTGGATCCAAACAAACCAATAAGGAAGC
  AGCTAAAGAGAGGATCACCTTACAATTTGAACTTTAGAGTCAAATTCTTTGTAAGTGACCCCAACAAGTT
  ACAAGAAGAGTATACAAGGTATCAGTACTTTTTGCAAATTAAGCAAGACATTCTTACTGGAAGATTATCC
  TGTCCTTGTAACACTGCTGCCCTTTTAGCATCATTTGCTGTTCAGTCTGAACTTGGAGACTACAATCAGT
  CAGAAAACTTGGCAGGCTACCTCTCAGATTATTCTTTCATTCCTAATCAACCTCAAGATTTTGAGAAAGA
  AATTGCAAAGTTACATCAGCAGCACGTTGGCCTATCTCCTGCAGAAGCAGAATTTAATTACCTAAACGCG
  GCACGTACCTTAGAACTCTATGGAGTTGAATTTCACTATGCAAGGGATCAAAGTAACAATGAAATCCTGA
  TTGGAGTGATGTCAGGAGGAATTCTGATTTATAAGAACAGGGTACGGATGAATACTTTTCTGTGGTTGAA
  GATTGTAAAAATTTCTTTTAAATGCAAACAGTTTTTTATTCAACTTAGAAAAGAGTTGCATGAATCTAGA
  GAAACATTACTGGGATTTAATATGGTGAATTATAGAGCATGTAAAACTTTGTGGAAAGCGTGTGTAGAAC
  ATCATACATTCTTCCGCCTGGATAGACCACTTCCACCTCAAAAAAATTTTTTTGCACATTATTTTACATT
  GGGTTCCAAATTCCGGTACTGTGGGAGAACTGAAGTCCAGTCAGTTCAATATGGCAAAGAAAAGGCAAAT
  AAAGACAGGGTATTTGCAAGATCTCCAAGTAAGCCTTTGGCACGGAAATTAATGGATTGGGAAGTAGTCA
  GCAGAAATTCATTATCTGATGACAGGTTAGAAACACAAAGCCTCCCATCCCGGTCTCCACCTGGAACTCC
  CAACCATCGGAATTCTTCATTCACACAAGAGGCAACCCGGGTTCGGCCGTCTTCAGTTGGTCATTTGGTA
  GACCATGTGGTTCACATGTCCCCCAGTGAGGATTTTGTAAGTCAGAGATCTCCATCATCAACGCAAGCTA
  ATAGCATAGTTCTGGAGTCATCACCATCACAAGAGACCCCTGAAGATGGGCAGCCACCAGCTTTACCACC
  CAAACAATCTAAGAAAAATAGTTGGAACCAAATTCATTTTTCAAACTCTCAGCAAGATCTAGTCACCCAT
  ACTAATGAATCCTTTGATGTGCCCTCTTCCCCTGAAAAGTCCACTCCTAATGGTGGCATTCCACATGATA
  ACCTTGTTCTAATCAAAATGAAACCTGATGAAAATGGAAGGTTTGGATTCAATGTAAAGGGAGGATATGA
  TCAGAAGATGCCTGTAATTGTTTCCCGGGTAGCACCAGGAACACCTGCTGACCTCTGTGTCCCTCGCTTG
  AATGAAGGGGACCAAGTGGTACTAATAAATGGTCGGGACATTGCAGAACATACCCATGATCAAGTAGTCT
  TGTTTATTAAAGCTAGCTGTGAGAAACATTCTGGGGAACTCGTGCTCCTAGTCCGACCTAATGCTGTATA
  TGATGTAGTGGAAGAAAAACTAGAAAGTGAACCAGACTTCCAGTATATTCCTGAGAAAGCCCCACTAGAT
  AGTGTCCATCAAGATGACCATTCCTTGCGGGAGTCAATGATCCAGCTAGCTGAGGGGCTTATCACTGGAA
  CAGTACTGGCACAGTTTGATCAACTCTATCGGAAAAAACCTGGAATGACAATGTCTTGTGCCAAATTACC
  TCAGAACATTTCCAAAAACAGATACAGAGATATTTCACCTTATGATGCTACACGGGTCCTTTTAAAAGGT
  AATGAAGACTACATCAATGCAAACTATATAAATATGGAAATTCCTTCTTCAAGTATTATAAATCAATACA
  TTGCTTGTCAAGGGCCATTACCACACACTTGTAAAGATTTTTGGCAAATGACTTGGGAACAAGGCTCCTC
  CATGGTTGTGATGTTGACCACACAAGTTGAACGTGGCAGAGTTAAATGTCACCAGTATTGGCCAGAACCC
  TCAGAAAGCTCATCCTATGGATGCTATCAAGTCACCTGCCACTCTGAAGAAGGAAACCCTGCCTATATCT
  TCAGGAAGATGACACTGTTTAACCAAGAGAAAAATGAGAGCCGTCAACTTACTCAGATTCAGTACACAGC
  CTGGCCTGACCATGGAGTACCTGATGATTCGAGTGACTTTCTGGATTTTGTTTGTCATGTACGAGACCAG
  AGGGCTGGAAAAGAAGAGCCCATTATTGTTCATTGCAGTGCTGGAATTGGAAGGACTGGGGTTCTTATTA
  CTATGGAAACTGCCATGTGTCTCATTGAATGCAATCAGCCAGTTTATCCACTAGACATTGTAAGAACAAT
  GAGAGATCAAAGAGCAATGATGATCCAAACACCTAGTCAATACAGATTTGTATGTGAAGCTATTCTGAAA
  GTTTATGAAGAAGGATTTGTTAAACCATTAACAACATCATCAAATAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019933
• Insert Size: 2781 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019933.2, NP_064317.2
• RefSeq Size: 4707 bp
• RefSeq ORF: 2781 bp
• Locus ID: 19258
• UniProt ID: Q9WU22
• Cytogenetics: 1 52.39 cM
• Gene Summary: Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis (PubMed:17953619, PubMed:25825441). Regulates neuronal cell homeostasis by protecting neurons against apoptosis (By similarity). Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction (PubMed:25825441). Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway (PubMed:18614237). May act at junctions between the membrane and the cytoskeleton.[UniProtKB/Swiss-Prot Function]