Npepps (NM_008942) Mouse Untagged Clone

CAT#: MC222632

Npepps (untagged) - Mouse aminopeptidase puromycin sensitive (Npepps), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Npepps
• Synonyms: AAP-S; goku; MP100; Psa; R74825
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222632 representing NM_008942
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGGCAGCCGCCGTCCCCTCCCTCGCTCGCCGCCTGCTCCTCCTCGGCCCTCCGCCTCCTCCCC
  TCCTTCTCCTCCTCAGCCGCTCCTCTCGCCGCCGCCGCCGCCTCCACAGCCTGGGCCTCGCCGCGATGCC
  GGAGAAGCGGCCCTTCGAGCGGCTGCCCGCCGAGGTGTCGCCCATCAACTACAGCCTCTGCCTCAAGCCC
  GATTTGCTGGACTTCACCTTCGAGGGCAAGCTGGAGGCCGCCGCCCAGGTAAGGCAGGCAACCAATCAAA
  TTGTGATGAATTGTGCTGATATTGACATTATTACAGCTTCATATGCCCCAGAAGGAGATGAAGAAATCCA
  TGCGACAGGATTTAACTATCAGAATGAAGATGAGAAAGTCACCTTGTCTTTTCCTAGCACTCTGCAAACA
  GGTACAGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGAAGCA
  GATACACCACCCCTGCCGGCGAGGTGCGCTATGCTGCTGTCACACAGTTTGAGGCTACTGATGCCCGAAG
  GGCTTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCGCTGGTTGTGCCTAAAGAC
  AGAGTGGCTTTATCAAATATGAATGTAATTGACAGGAAACCATATCCTGATGATGAAAATTTAGTGGAAG
  TGAAGTTTGCTCGCACACCTGTTATGTCTACGTATCTGGTGGCATTTGTTGTGGGTGAATATGACTTTGT
  AGAAACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACCCCTGTTGGCAAAGCAGAGCAAGGAAAG
  TTTGCGCTCGAGGTTGCTGCTAAGACCTTGCCTTTTTATAAAGACTACTTCAATGTTCCTTATCCTCTAC
  CTAAAATTGATCTCATTGCTATTGCTGACTTTGCAGCTGGTGCCATGGAGAACTGGGGCCTTGTTACTTA
  TAGGGAAACGGCCTTGCTTATTGATCCAAAAAACTCGTGTTCTTCATCACGCCAGTGGGTTGCTCTGGTT
  GTGGGACATGAACTCGCCCATCAATGGTTTGGAAATCTTGTTACTATGGAATGGTGGACTCATCTCTGGT
  TGAATGAAGGCTTTGCATCCTGGATTGAGTATCTTTGTGTAGACCACTGCTTTCCAGAGTATGATATCTG
  GACTCAGTTTGTTTCTGCAGATTATACCCGTGCCCAGGAACTTGATGCCTTAGATAACAGCCATCCTATT
  GAAGTCAGTGTGGGCCATCCGTCTGAGGTTGATGAGATATTTGATGCTATATCATATAGCAAAGGTGCAT
  CTGTAATCCGAATGCTACATGACTACATTGGTGATAAGGACTTTAAGAAAGGAATGAATATGTATTTAAC
  CAAGTTCCAACAAAAGAATGCTGCCACAGAGGATCTCTGGGAAAGTTTGGAAAGTGCCAGTGGCAAACCC
  ATAGCAGCTGTGATGAATACCTGGACCAAACAAATGGGATTCCCTCTCATTTATGTGGAAGCTGAACAGG
  TAGAAGATGACAGAGTGCTGAAGCTGTCTCAGAAGAAGTTTTGTGCCAGTGGACCATATGGCGGTGAAGA
  CTGTCCTCAGTGGATGGTTCCTATCACAATTTCAACTAGTGAGGATCCTAACCAGGCTAAGCTGAAAATA
  CTAATGGATAAGCCAGAGATGAGTGTGGTTTTGAAAAATGTCAAACCAGACCAATGGGTAAAGCTAAATC
  TGGGAACAGTTGGGTTTTATCGAACCCAGTACAGCTCTGCCATGCTCGAAAGTTTATTACCAGGCATCCG
  TGACCTTTCTCTGCCCCCAGTGGATCGACTTGGATTACAGAATGACCTCTTTTCTCTGGCTCGAGCTGGC
  ATCATTAGCACTGTAGAGGTTCTAAAAGTCATGGAGGCTTTTGTGAATGAGCCCAATTATACTGTATGGA
  GCGACCTGAGCTGTAACCTGGGGATTCTTTCAACTCTCTTGTCCCACACAGACTTCTATGAGGAAATCCA
  GGAGTTTGTCAAAGATGTCTTTTCACCTATAGGAGAGAGATTGGGCTGGGACCCCAAACCTGGAGAAGGT
  CATCTAGACGCACTCCTGAGGGGCTTGGTGCTGGGCAAACTGGGAAAAGCAGGCCATAAGGCAACTTTGG
  AAGAAGCCCGTCGTCGGTTTAAGGAGCACGTGGAAGGGAAACAGATTCTTTCTGCTGACCTAAGGAGTCC
  TGTCTATCTCACTGTTTTAAAGCATGGGGATGGCGCTACCTTAGATATCATGCTGAAGCTTCACAAACAA
  GCTGATATGCAAGAAGAGAAAAACAGAATTGAAAGAGTTCTTGGGGCTACTCTTTCACCTGAACTGATTC
  AAAAAGTCCTTACTTTTGCACTTTCAGAAGAGGTCCGTCCGCAGGACACTGTGTCGGTGATTGGTGGAGT
  GGCTGGAGGCAGCAAGCATGGGAGGAAAGCTGCTTGGAAATTCATCAAGGACAACTGGGAGGAGCTTCAC
  AACCGGTACCAGGGAGGGTTCTTAATATCCAGACTAATAAAGCTCTCAGTTGAGGGGTTTGCAGTTGATA
  AAATGGCTGGAGAAGTTAAGGCTTTCTTCGAGAGTCACCCAGCTCCTTCAGCTGAGCGCACCATCCAGCA
  GTGTTGTGAAAATATCCTGTTGAATGCTGCTTGGCTCAAGCGAGATGCTGACAGCATTCACCAGTACCTC
  CTTCAGCGGAAAACCTCCCCACCCTCGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008942
• Insert Size: 2763 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008942.2, NP_032968.2
• RefSeq Size: 4161 bp
• RefSeq ORF: 2763 bp
• Locus ID: 19155
• UniProt ID: Q11011
• Cytogenetics: 11 60.95 cM
• Gene Summary: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins.[UniProtKB/Swiss-Prot Function]