Nsmaf (NM_010945) Mouse Untagged Clone

CAT#: MC222630

Nsmaf (untagged) - Mouse neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsmaf
• Synonyms: AA959567; C630007J05; Fan
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222630 representing NM_010945
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCACCCGGAAGAGGCAGCGCGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGGTTTTCCTTAC
  TGCTTCTCAACTTGGAGGAGTACTACTTTGAGCAGCACACAGCGTTCCATGTCCAGCACCAGGGCAGCCA
  GGAAGAAAGGAAAATCAGAGGCTCCTTAAAGATATGTTCAAAATCAGTGATTTTTGAACCAGATGCAATA
  TCTCAGCCCATTCTTAAGATTCCTTTGAGAGACTGTTTAAAAATAGGAAAACATGGAGAAAATGGAGCCA
  ATAAACACTTTGCAAAGGCAAAATCATGGGGTATTTCACTCATTTTCAGTCAGATATACTTTATTAAGGA
  GCACAACATTGTGGCACCGTATAAGATAGAGCGGGGAAAAATGGAGTATGTCTTTGAACTGGAAGTTTCT
  GGGAAGGTGGAAGATGTTGTGGAGACATTGCTACAGCTGCACAGAGCATCCTGCCTTGATAAACTTGGTG
  ACCAAATGGCCATGATAACAGCTATCTTGCAGTCACGTCTGGCTAGGACATCATTTGACAAAAACAGGTT
  CCAAAGTGTTTCTGAAAAGCTGCACATGGAGTGCAAAGCAGAGATGGTAACACCTCTAGTGACGAACCCT
  GGGCATGTGTGCATTACAGACACCAGCCTGTATTTCCAGCCTCTCAATGGGTACCCGAAACCTGTGGTCC
  AGATAACACTGCAGGATGTCCGGCGCATTTACAAAAGGAGGCACGGCCTCATGCCTTTGGGCTTGGAAGT
  GTTTTGCACAGATGACGACCTGTGTTCCGACATATACCTAAAGTTCTATGAGCCTCAAGACAGAGATGAT
  CTCTATTTTTATATCGCCACGTACCTAGAACACCACGCTGCAGAACACACTGCGGAGAGCTACATGCTGC
  AGTGGCAGCGAGGGCACCTTTCCAACTACCAGTACCTGCTCCACCTCAACAACCTGGCCGACCGCAGCTG
  CAATGACCTGTCCCAGTACCCTGTGTTTCCATGGATAATAAGTGATTACTCCAGCCCAGAGCTAGATTTG
  TCAAATCCTGCAACCTTCCGAGATCTTAGTAAGCCAGTGGGAGCCCTAAATGCGGAGCGACTGGAGAGAC
  TGCTGACTCGATACCAGGAAATGCCTGAGCCAAGATTCATGTATGGGAGTCACTACTCTTCTCCAGGCTA
  TGTGCTCTTTTACCTCGTTAGGATCGCACCAGAGTATATGCTGTGTCTACAAAATGGAAGATTTGATAAT
  GCAGATAGGATGTTTAACAGTATTGCAGAAACGTGGAAAAACTGTTTGGATGGTGCAACGGATTTTAAAG
  AGTTGATTCCAGAATTCTATGATGAAGACGTCAGCTTTTTGGTCAATAGTCTGAAGCTGGATCTGGGAAA
  GAGACAAGGCGGGCAGATGGTTGATGACGTGGACCTTCCAGCCTGGGCCTCAAGTCCCCAAGACTTCCTG
  CAGAAGAACAAGGATGCCTTGGAAAGCGGTTACGTGTCTGAGCACCTTCATGAGTGGATTGATCTGATAT
  TTGGCTATAAGCAAAAAGGGAGTGAGGCCATAGGGGCCCATAACGTGTTTCATCCCCTGACCTATGAAGG
  TGGTGTAGACTTGAACAGCATTGAGGATCCTGATGAAAAAGTAGCCATGCTGACCCAAATTTTGGAATTT
  GGGCAGACACCAAAACAGCTGTTTGTGACACCACATCCTCGAAGGATCACCCCCAAGTTTAAAAGTTTGT
  CCCAAGCCTCCAGCTACAACGCTTCTCTGACAGACTCCCCAGTTTCTCCAGGGGAGGAGTCGTTTGAAGA
  CCTGACTGAGGAAAGCAGAACTCTGGCCTGGAGTAATATCGCCAAGCTGCAGCTACATGAGCAGTACAAG
  ATCCACAAAGAGGCGGTTACAGGGATAGCAGTGTCTTGCAATGGCTCTTCAGTCTTTACCACCTCCCAAG
  ATTCCACTTTGAAGATGTTTTCTAAGGAATCCAAAATGCTACAAAGAAGTATATCATTTTCCAATATGGC
  TTTATCCTCTTGTTTACTGTTGCCGGGAGACACCACTGTCATAAGTTCTTCTTGGGATAACAACGTCTAT
  TTTTATTCCATAGCATTTGGAAGACGCCAAGATACACTGATGGGACATGACGATGCTGTCAGTAAGATCT
  GTTGGCATAACGACAGGCTGTACTCTGGATCTTGGGACTCTACTGTGAAGGTGTGGTCCGGTGTTCCTGC
  AGAAATGCCAGGAACAAAAAGACACCAGTTTGACTTACTGGCTGAGCTTGAACATGATGTCAGCGTCAAT
  ACTATCAATTTAAATGCTGTGAGCACACTGTTAGTTTCTGGCACCAAAGAAGGCATGGTGAATATTTGGG
  ACCTCACTACAGCCACTTTATTACACCAGACTTCATGCCATTCAGGGACAGTATGTGATGCTGCTTTTAG
  CCCAGATAGTCGCCACATCCTCAGCACGGGCGTAGATGGATGTCTCAATGTGATTGATGTGCAGACGGGC
  ATGCTCATCTCCTCCATGGCCTCAGAGGAGCCGCAGAGGTGCTTTGTCTGGGATGGAAATTCTGTCTTAT
  CTGGAAGTCGGTCTGGTGAGCTGCTTGTTTGGGACCTCCTTGGAGCAAAAGTCAGTGAGCGGATTCAGGG
  CCACACTGGTGCTGTGACATGCATGTGGATGAACGAACAGTGTAGCAGTATTATCACCGGAGGGGAAGAC
  AGACAAATTATGTTCTGGAAATTGCAGTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010945
• Insert Size: 2763 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010945.2, NP_035075.2
• RefSeq Size: 3518 bp
• RefSeq ORF: 2763 bp
• Locus ID: 18201
• UniProt ID: O35242
• Cytogenetics: 4 A1
• Gene Summary: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.[UniProtKB/Swiss-Prot Function]