Tpo (NM_009417) Mouse Untagged Clone

CAT#: MC222583

Tpo (untagged) - Mouse thyroid peroxidase (Tpo), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tpo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222583 representing NM_009417
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACACTTGGAGCTATGGCAATAATGCTGGTGGTTATGGGAACTGTAATTTTCCTCTCTTTTATCC
  TGAGAAGCAGAGACATCTTGTGTGGGAAGACCATGAAGTCCCATGTTATCAGTGCTGTGGAAACGAGCCA
  GCTCATGGTGGACCATGCAGTCTACAACACCATGAAAAGAAACCTCAAGAAAAGGGAAGTCCTTTCTCCA
  GCCCAGCTTCTCTCTTTCTTTAAGCTGCCCGAGTCCACCAGTGGGGCTATTTCCCGAGCAGCAGAGATTA
  TGGAAACATCAATACAAGTCATGAAACGTGAACAGTCACAGTTCTCCACGGATGCCTTATCAGCAGACAT
  TCTGGGCACAATTGCCAACCTGTCAGGATGCTTGCCTTTCATGCTGCCACCAAGATGTCCTGACACCTGC
  CTGGCAAATAAGTACCGGCCCATCACAGGGGCGTGCAACAATAGAGATCACCCCAGATGGGGAGCCTCCA
  ACACAGCCCTAGCAAGATGGCTGCCTCCTGTCTATGAAGATGGCTTCAGTCAGCCCAAAGGCTGGAACCC
  TAATTTCTTATACCACGGCTTCCCACTGCCCCCGGTACGGGAAGTGACAAGGCACCTCATTCAAGTTTCC
  AATGAGGCTGTGACCGAAGATGACCAGTACTCTGATTTTCTGCCGGTGTGGGGACAGTACATCGATCATG
  ACATTGCTCTCACACCACAGAGCACTAGCACAGCAGCCTTCTGGGGAGGTGTCGACTGCCAGCTGACCTG
  TGAGAACCAAAATCCTTGCTTCCCCATACAGCTTCCCTCAAACTCCTCAGGGACCACTGCATGCCTGCCT
  TTCTACCGCTCCTCCGCCGCTTGTGGCACTGGGGACCAAGGTGCTCTCTTTGGCAACCTGTCTGCAGCCA
  ATCCGAGGCAGCAGATGAATGGCTTGACCTCCTTCCTTGATGCTTCCACTGTGTACGGCAGCTCCCCTGG
  CGTTGAGAAGCAGCTGCGCAACTGGAGCAGCTCGGCAGGACTGCTGCGTGTCAACACTCTCCACCTAGAT
  GCTGGCCGTGCCTACCTGCCCTTCGCAACAGCCGCCTGCGCTCCAGAGCCTGGTACCCCACGCACCAACC
  GCACGCCCTGCTTCCTGGCTGGAGACGGTCGCGCCAGTGAGGTCCCTGCCCTGGCAGCGGTACACACCTT
  GTGGCTGCGCGAGCACAACCGCCTGGCTTCGGCCTTCAAGGCCATTAACAAGCACTGGAGCGCCAACACT
  GCCTACCAGGAGGCGCGCAAGGTGGTAGGGGCACTGCACCAGATCATCACCATGAGGGATTATATCCCCA
  AAATCCTGGGTCCTGATGCCTTCAGGCAGTATGTGGGCCCCTATGAAGGCTACAACCCCACTGTGAACCC
  TACTGTGTCCAACATCTTCTCCACTGCTGCCTTTCGCTTTGGCCATGCCACAGTCCATCCACTGGTGAGA
  CGGCTAAACACTGACTTCCAGGAGCACACAGAGCTCCCCAGGTTGCAGCTGCGTGATGTCTTCTTCAGAC
  CCTGGAGGCTTATCCAGGAAGGTGGTTTGGATCCGATAGTGAGAGGCCTCCTGGCGAGAGCAGCCAAGCT
  GCAAGTCCAAGGGCAGCTGATGAATGAGGAGCTGACCGAGAGGCTCTTCGTGCTGTCTAACGTGGGCACC
  TTGGATCTGGCATCACTGAACTTGCAGAGGGGCCGGGATCATGGCTTACCAGACTACAATGAATGGAGAG
  AGTTCTGTGGCTTGTCACGCCTGGAGACACCAGCTGAGCTGAACAAGGCCATTGCCAACAGAAGCATGGT
  CAACAAGATAATGGACTTATACAAGCATGCTGACAACATTGACGTCTGGTTGGGAGGCTTGGCTGAAAAG
  TTCTTGCCGGGGGCCCGCACTGGTCCTCTGTTTGCATGTATCATTGGGAAGCAGATGAAGGCTCTGAGGG
  ATGGGGACAGGTTTTGGTGGGAGAACACCAATGTCTTCACAGACGCTCAGAGGCAGGAACTAGAAAAGCA
  TTCACTACCTCGGGTCATCTGTGACAACACTGGACTCACCAGAGTACCTGTGGATGCCTTCCGTATTGGA
  AAGTTCCCCCAAGACTTTGAATCCTGTGAGGACATCCCGAGCATGGACCTTGAACTATGGAGGGAGACCT
  TCCCACAAGATGACAAGTGTGTCTTCCCAGAGGAGGTGGACAATGGGAACTTTGTGCACTGTGAAGAGTC
  TGGGAAGCTGGTGCTTGTATACTCCTGTTTCCATGGATACAAGCTGCAGGGCCAGGAGCAGGTCACATGT
  ACCCAGAAGGGATGGGACTCCGAACCTCCTGTCTGTAAAGATGTTAATGAGTGTGCAGATCTGACACACC
  CACCTTGCCACCCCTCTGCACAGTGCAAGAACACCAAGGGAAGCTTCCAGTGTGTGTGCACAGACCCCTA
  TGTGCTAGGTGAGGATGAGAAGACCTGCATAGATTCTGGCAGGCTACCTCGGGCATCCTGGGTCTCCATT
  GCACTGGGTGCACTTCTCATTGGTGGTTTGGCCAGTCTCACCTGGATAGTAATTTGCAGGTGGACACATG
  CTGATAAGAAAGCCACATTGCCGATCACAGAGAGAGTGACCACCCAGTCAGGATGCAGAAAGAGTCAGGG
  GAGGGGGATTTCACCACACAAGGCCGCAGCTCAAGACACTGGACAGGAACCTGCAAGTGGATCCAGGGTC
  CTCCTGTGCGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009417
• Insert Size: 2745 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009417.3, NP_033443.1
• RefSeq Size: 3306 bp
• RefSeq ORF: 2745 bp
• Locus ID: 22018
• UniProt ID: P35419
• Cytogenetics: 12 13.0 cM
• Gene Summary: This gene encodes a membrane-bound glycoprotein. The encoded enzyme plays a central role in thyroid gland function. The enzyme functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mice with homozygous missense mutations in this gene exhibit hypothyroid dwarfism and hearing impairment. [provided by RefSeq, Sep 2015]