Tlr3 (NM_126166) Mouse Untagged Clone

CAT#: MC222546

Tlr3 (untagged) - Mouse toll-like receptor 3 (Tlr3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlr3
• Synonyms: AI957183
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222546 representing NM_126166
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGGGTGTTCCTCTTATCTAATGTACTCCTTTGGGGGACTTTTGTCCCTATGGATTCTTCTGGTGT
  CTTCCACAAACCAATGCACTGTGAGATACAACGTAGCTGACTGCAGCCATTTGAAGCTAACACACATACC
  TGATGATCTTCCCTCTAACATAACAGTGTTGAATCTTACTCACAACCAACTCAGAAGATTACCACCTACC
  AACTTTACAAGATACAGCCAACTTGCTATCTTGGATGCAGGATTTAACTCCATTTCAAAACTGGAGCCAG
  AACTGTGCCAAATACTCCCTTTGTTGAAAGTATTGAACCTGCAACATAATGAGCTCTCTCAGATTTCTGA
  TCAAACCTTTGTCTTCTGCACGAACCTGACAGAACTCGATCTAATGTCTAACTCAATACACAAAATTAAA
  AGCAACCCTTTCAAAAACCAGAAGAATCTAATCAAATTAGATTTGTCTCATAATGGTTTATCATCTACAA
  AGTTGGGAACGGGGGTCCAACTGGAGAACCTCCAAGAACTGCTCTTAGCAAAAAATAAAATCCTTGCGTT
  GCGAAGTGAAGAACTTGAGTTTCTTGGCAATTCTTCTTTACGAAAGTTGGACTTGTCATCAAATCCACTT
  AAAGAGTTCTCCCCGGGGTGTTTCCAGACAATTGGCAAGTTATTCGCCCTCCTCTTGAACAACGCCCAAC
  TGAACCCCCACCTCACAGAGAAGCTTTGCTGGGAACTTTCAAACACAAGCATCCAGAATCTCTCTCTGGC
  TAACAACCAGCTGCTGGCCACCAGCGAGAGCACTTTCTCTGGGCTGAAGTGGACAAATCTCACCCAGCTC
  GATCTTTCCTACAACAACCTCCATGATGTCGGCAACGGTTCCTTCTCCTATCTCCCAAGCCTGAGGTATC
  TGTCTCTGGAGTACAACAATATACAGCGTCTGTCCCCTCGCTCTTTTTATGGACTCTCCAACCTGAGGTA
  CCTGAGTTTGAAGCGAGCATTTACTAAGCAAAGTGTTTCACTTGCTTCACATCCCAACATTGACGATTTT
  TCCTTTCAATGGTTAAAATATTTGGAATATCTCAACATGGATGACAATAATATTCCAAGTACCAAAAGCA
  ATACCTTCACGGGATTGGTGAGTCTGAAGTACCTAAGTCTTTCCAAAACTTTCACAAGTTTGCAAACTTT
  AACAAATGAAACATTTGTGTCACTTGCTCATTCTCCCTTGCTCACTCTCAACTTAACGAAAAATCACATC
  TCAAAAATAGCAAATGGTACTTTCTCTTGGTTAGGCCAACTCAGGATACTTGATCTCGGCCTTAATGAAA
  TTGAACAAAAACTCAGCGGCCAGGAATGGAGAGGTCTGAGAAATATATTTGAGATCTACCTATCCTATAA
  CAAATACCTCCAACTGTCTACCAGTTCCTTTGCATTGGTCCCCAGCCTTCAAAGACTGATGCTCAGGAGG
  GTGGCCCTTAAAAATGTGGATATCTCCCCTTCACCTTTCCGCCCTCTTCGTAACTTGACCATTCTGGACT
  TAAGCAACAACAACATAGCCAACATAAATGAGGACTTGCTGGAGGGTCTTGAGAATCTAGAAATCCTGGA
  TTTTCAGCACAATAACTTAGCCAGGCTCTGGAAACGCGCAAACCCCGGTGGTCCCGTTAATTTCCTGAAG
  GGGCTGTCTCACCTCCACATCTTGAATTTAGAGTCCAACGGCTTAGATGAAATCCCAGTCGGGGTTTTCA
  AGAACTTATTCGAACTAAAGAGCATCAATCTAGGACTGAATAACTTAAACAAACTTGAACCATTCATTTT
  TGATGACCAGACATCTCTAAGGTCACTGAACCTCCAGAAGAACCTCATAACATCTGTTGAGAAGGATGTT
  TTCGGGCCGCCTTTTCAAAACCTGAACAGTTTAGATATGCGCTTCAATCCGTTCGACTGCACGTGTGAAA
  GTATTTCCTGGTTTGTTAACTGGATCAACCAGACCCACACTAATATCTCTGAGCTGTCCACTCACTACCT
  CTGTAACACTCCACATCATTATTATGGCTTCCCCCTGAAGCTTTTCGATACATCATCCTGTAAAGACAGC
  GCCCCCTTTGAACTCCTCTTCATAATCAGCACCAGTATGCTCCTGGTTTTTATACTTGTGGTACTGCTCA
  TTCACATCGAGGGCTGGAGGATCTCTTTTTACTGGAATGTTTCAGTGCATCGGATTCTTGGTTTCAAGGA
  AATAGACACACAGGCTGAGCAGTTTGAATATACAGCCTACATAATTCATGCCCATAAAGACAGAGACTGG
  GTCTGGGAACATTTCTCCCCAATGGAAGAACAAGACCAATCTCTCAAATTTTGCCTAGAAGAAAGGGACT
  TTGAAGCAGGCGTCCTTGGACTTGAAGCAATTGTTAATAGCATCAAAAGAAGCCGAAAAATCATTTTCGT
  TATCACACACCATTTATTAAAAGACCCTCTGTGCAGAAGATTCAAGGTACATCACGCAGTTCAGCAAGCT
  ATTGAGCAAAATCTGGATTCAATTATACTGATTTTTCTCCAGAATATTCCAGATTATAAACTAAACCATG
  CACTCTGTTTGCGAAGAGGAATGTTTAAATCTCATTGCATCTTGAACTGGCCAGTTCAGAAAGAACGGAT
  AAATGCCTTTCATCATAAATTGCAAGTAGCACTTGGATCTCGGAATTCAGCACATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_126166
• Insert Size: 2718 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_126166.4, NP_569054.2
• RefSeq Size: 4327 bp
• RefSeq ORF: 2718 bp
• Locus ID: 142980
• UniProt ID: Q99MB1
• Cytogenetics: 8 B1.1
• Gene Summary: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function]