E2f7 (NM_178609) Mouse Untagged Clone

CAT#: MC222527

E2f7 (untagged) - Mouse E2F transcription factor 7 (E2f7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E2f7
• Synonyms: A630014C11Rik; D10Ertd739e; E2F-7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222527 representing NM_178609
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGAATTGTTTAACACTAAAAGACTTGATCAGCCCCAGGCAGACCAGACTAGATTTTGCCATTG
  AAGATGCAGAGAACGCACAAAAGGAAAATATATTTGTCGACCGATCAAGGATGACCCCAAAGACACCGAT
  GAAGAACGAGCCGATCGACCTGTCAAAGCAAAGAATCTTCACCCCAGACAGAAACCCCATTACTCCAGTA
  AAGCCGGTCGACAGGCAGCCGCAGGTGGAGCCCTGGACACCCACAGCCAACCTGAAGATGCTCATCAGCG
  CCGCCAGCCCAGACATAAGAGACCGGGAGAAGAAAAAGGAGCTGTTCAGACCCATTGAGAATAAGGAGGA
  TGCGTTCGTGAACTCCCTGCAGCTTGACGTGGCCGGTGACGGCGCTGTGGATGAGTATGAGAAGCAAAGG
  CCAAGCAGGAAACAGAAGAGCTTGGGGCTGCTGTGCCAGAAGTTTCTAGCTCGCTATCCGAGCTACCCCT
  TGTCAACGGAGAAAACCACCATCTCCCTAGATGAGGTCGCCGTCAGTCTCGGCGTGGAAAGGAGACGCAT
  CTATGACATCGTAAACGTGCTGGAGTCGCTGCATCTGGTCAGCCGGGTGGCTAAGAATCAGTATGGTTGG
  CACGGTCGGCACAGCCTCCCCAAAACCCTGAGGACCCTACAGAGACTGGGAGAGGAACAGAAATACGAGG
  AGCAGATGGCCTGCCTCCAGCAGAAGGAGCTGGACCTGATGGGGTATAGGTTCGGAGAACGCAGGAAAGA
  CGGGTCTCCGGACCCCCGAGATCCACACCTACTTGATTTTTCCGAAGCCGACTACCCCTCTTCATCTGCA
  AACAGTCGAAAAGATAAATCTTTAAGAATTATGAGCCAGAAGTTTGTCATGCTGTTCCTCGTCTCCAAAA
  CCAAGATTGTCACCCTGGATGTAGCTGCCAAAATCCTCATAGAAGAAAGCCAAGATACCCCGGACCACAG
  CAAATTCAAAACAAAGGTACGGAGGCTCTATGACATAGCCAATGTTCTGACCAGCCTGGCTCTGATAAAG
  AAAGTTCACGTAACAGAAGAGCGAGGCCGGAAACCAGCCTTCAAGTGGATCGGGCCTGTGGACTTCAGCT
  CCATTGATGAAGAACTGTTGGACGTCTCTGCATCCATCTTACCAGAATTGAAGAAGGAAGCATACGGCCA
  GATCCGAGTGTGTGCAAAAGAGAGGCTGGTGCGGTACGGTTCCTTTAACACGGTTCACACGTCTGAGAAG
  ATACAGAGAAAAGTGAGCTCAGAACCCAGCAGCCCACAGGGAGAAAGACAAGGGTCAGCCTATTCCCTGG
  AAATTGGAAGTCTGGCGGCCATCTACAGACAGAAAGTAGAAGATAATTCGCAGGAGGAAGCCTTTGTCAG
  TAACACAGCAGTGCCTCCAGCAAGCATCTTGGACCCCGCCCTCTCCATGGACTCTGAATACTGTGTCAAG
  CCTTTAGCCCAGCCAGTATTTTCTGTTGCTCAAACAGATTTGCCGGCCTTCTCTGCGCAGAATGGTCCAA
  GCGGACAAGTAGGTGTCCCCGTTCCTTCTGCAGCCTCCGACACAGAGAACTTAAAGCCAGCTCTGCTCGC
  CGGCCAGCCCCTGGTCTACGTGCCCTCCACCCAACTCTTCATGCTGTACGGGAGTGTGCAGGAGGGACTG
  TCCCCAGAGTCAAGGTCAGAGGAAGATGGTGGTGGCTCCGATGTCCCAGCTGACCTGTCAGTGACACCCT
  CTGCTCAGAAGCGCCTGTGTGAGGAGAGAGATCCTCAGGAGGAGGAGGACGAGCCCGCCATGAAAAGACA
  AAGTCAGGAATTTGAAGACAGCCCCTTATCCCTAGTTATGCCCAAGAAACCCTCAAGTTCCACAGACCTT
  GCCTGTCCTGTGACCATGGGAAACGGGAGCTCTCCACCCCTGGAAGACGCCTGTGTTAAGGGTCAGCTCC
  CTGCTGCAGAAGAAGTTACAGGAAAGGCTGCCCCAAACTGCTATGTTGCTTCTGAGTGTGGAAACCCGGC
  AAGAAATCCAGACACTGAAAAGCCTTCCAACGAAAACGAGATCACCAAAGACCCCTCGCTGATGCAGTAC
  CTGTATGTGCAGTCTCCCGCAGGGTTAAATGGTTTTAACATGGTTTTACCAGGCACCCAGACCCCCCACA
  CCGTGGCACCCTCCCCGGCTCAGCTGCCATCCTTCGGCGTACCCTGCATGTTCCTGCAATCCCCAGGCCT
  GGGCCCTTTTCCAGTTCTCTATTCTCCCGCAATACCCGGGCCCATTTCTTCAGCCCCCGGCACTCACCCA
  AACCCCGGACCCATGAACTTCGGCTTGTCCACCCTGGCATCTGCTTCTCACCTTCTCATCAGCCCGGCAG
  CCATGGTCAATCCAAAGCCATCCACCCTCCCCTGTACAGACCCTCAGCTTCGCTGTCAGCCCTCACTCAA
  CCTGAACCCCGTGATGCCAGGCTCACACGGCGTCATCCACCCCGAGTCCCCCTGTTACGTGAGACATCCG
  GTATCGATGGTAAAGGCAGAGCAGTCCCCTGCACCGGCGACTCCCAAGAGCATCCAACGGAGACATCGGG
  AGACGTTCTTCAAGACTCCCGGCAGCCTCGGCGATCCGGTCTTTAGAAGAAAAGAAAGGAACCAATCAAG
  AAACACTAGCTCAGCCCAGAGGAGACTAGAAATCTCTAGCAGCGGGCCTGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178609
• Insert Size: 2715 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178609.4, NP_848724.2
• RefSeq Size: 5476 bp
• RefSeq ORF: 2715 bp
• Locus ID: 52679
• UniProt ID: Q6S7F2
• Cytogenetics: 10 57.74 cM
• Gene Summary: Atypical E2F transcription factor that participates in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1. Acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Also involved in DNA damage response: up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dependent repression by mediating repression of indirect p53/TP53 target genes involved in DNA replication. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. Acts as a negative regulator of keratinocyte differentiation.[UniProtKB/Swiss-Prot Function]