Adamts8 (NM_013906) Mouse Untagged Clone

CAT#: MC222526

Adamts8 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts8
• Synonyms: METH-2; METH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222526 representing NM_013906
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGCGACCCCACCACCACCGGGTGGCCGCCCCTCCTGCTGCTGCTATTGCAGCTGCCGCCGCCGC
  CACTCGTCTGCGGCGCCCCGGCGGGGCCGGGAACCGGGGCGCAGGCCTCGGAGCTAGTGGTGCCCACGCG
  GTTGCCCGGCAGCGCGAGCGAGCTCGCCTTCCACCTGTCCGCCTTCGGCCAGGGCTTCGTGCTGCGCCTG
  GCGCCTGACGCCAGCTTCCTGGCGCCGGAATTCAAGATCGAGCGCCTCGGGGGCTCGAGCGCGGCGGCCG
  GGGGCGAGCCGGGACTGCGTGGCTGCTTCTTCTCTGGCACAGTGAATGGAGAACGGGAGTCGCTGGCGGC
  GATGAGCCTGTGTCGCGGGCTGAGCGGCTCGTTCTTGCTGGCAGGCGAGGAGTTCACCATCCAGCCACAG
  GGCGCTGGGGACTCCCTGGACCAGCCTCATCGCCTGCAGCGCTGGGGGCCGGGACAGCGCCGCGAAGACC
  CCGGGCTCGCTGCCGCCGAAGTTTTCCCCCTCCCTCAAGGACTGGAGTGGGAGGTGGAGATGGGTAATGG
  GCAGGGACAGGAGAGAAGTGACAACGAAGAGGACAGGAAGCAGGACAAGGAGGGGTTGCTCAAAGAGACA
  GAAGACTCCCGCAAAGTGCCACCACCCTTCGGATCCAAAACTAGAAGCAAGAGGTTTGTGTCCGAGGCTC
  GCTTCGTGGAAACACTTCTGGTGGCTGATGCGTCCATGGCTGCCTTCTATGGGACCGACCTGCAGAACCA
  CATCCTCACGGTGATGTCAATGGCAGCCCGAATCTACAAGCACCCGAGCATCAGGAACTCCGTCAACCTT
  GTGGTGGTGAAAGTGCTAATAGTGGAAGAGGAAGGATGGGGCCCGGAGGTGTCGGACAACGGGGGGCTCA
  CACTGCGCAACTTCTGCAGCTGGCAACGGCGTTTCAACAAGCCCAGTGACCGCCACCCGGAGCACTATGA
  CACTGCCATCTTGTTCACCAGACAGAACTTCTGTGGGAAGGGAGAGCAGTGTGACACCCTGGGGATGGCA
  GACGTTGGCACCATCTGTGACCCCGACAAGAGCTGCTCAGTGATCAAGGATGAGGGACTGCAGGCAGCCT
  ACACCCTGGCCCATGAGCTAGGGCACGTTCTCAGCATGCCCCATGATGATTCTAAGCCCTGTGTGAGATT
  GTTTGGGCCCATGGGCAAGTACCACATGATGGCGCCATTCTTCATCCACGTGAACAAGACGCTGCCCTGG
  TCTCCCTGCAGTGCTGTCTACCTCACAGAGCTCCTGGATGATGGTCACGGAGATTGTCTTCTGGATGCCC
  CCACCTCGGTTCTGCCCCTCCCCACAGGCCTCCCGGGCCACAGCACCCTCTACGAGCTGGACCAGCAGTG
  CAAGCAGATCTTTGGGCCTGATTTCCGACACTGCCCCAACACCTCTGTGGAGGACATCTGTGTCCAGCTC
  TGGTGCCGTCATCGGGATAGTGATGAGCCCATTTGCCACACAAAGAATGGTAGCCTGCTCTGGGCTGATG
  GTACACCCTGTGGCCCTGGGCACCTGTGCCTGGATGGTAGCTGTGTACTCAAGGAGGATGTGGAGAATCC
  CAAGGCTGTGGTAGATGGAGACTGGGGTCCCTGGAGACCCTGGGGACAATGTTCTCGCACCTGTGGTGGA
  GGGATACAATTCTCGAACCGTGAATGTGATAATCCAATGCCTCAGAATGGAGGAAGATTTTGCCTGGGTG
  AAAGAGTCAAGTACCAATCATGCAACACAGAGGAATGTCCACCAAACGGAAAAAGCTTCCGGGAGCAGCA
  GTGTGAGAAATATAATGCCTACAACCACACTGACCTGGATGGGAATTTCCTGCAGTGGGTCCCCAAGTAT
  TCAGGAGTGTCCCCCCGAGACCGATGCAAGCTGTTTTGCAGAGCCCGTGGGAGGAGTGAGTTCAAAGTGT
  TTGAAGCTAAGGTGATCGATGGCACTCTGTGTGGACCGGATACTCTGTCCATCTGCGTCCGGGGGCAATG
  TGTTAAGGCTGGCTGTGACCATGTGGTGAACTCACCTAAGAAGCTGGACAAATGTGGGGTGTGTGGGGGC
  AAAGGCACTGCCTGTAGGAAGATCTCCGGTTCTTTCACCCCCTTCAGTTATGGCTACAATGACATTGTCA
  CCATCCCAGCTGGTGCCACAAACATTGATGTGAAACAGCGGAGTCACCCAGGGGTCAGGAACGACGGCAG
  CTACCTGGCGCTGAAGACAGCCAATGGGCAGTACCTGCTCAATGGTAACCTGGCCATCTCTGCCATAGAG
  CAAGACATCTTGGTGAAGGGGACCATCCTGAAGTACAGTGGCTCCATGGCTACCCTGGAGCGGCTGCAGA
  GCTTCCAGGCCCTGCCTGAGCCTCTTACAGTACAGCTCCTGACTGTGTCTGGTGAGGTCTTCCCTCCAAA
  AGTCAGATATACCTTCTTTGTCCCCAATGACATGGACTTCAGCGTGCAGAATAGCAAGGAAAGAGCAACC
  ACCAACATCATTCAGTCACTGCCCTCTGCGGAGTGGGTTCTGGGAGACTGGTCTGAATGTCCGAGCACGT
  GCAGAGGTAGCTGGCAGCGGCGGACTGTGGAATGCAGGGACCCCTCAGGTCAGGCCTCTGACACCTGTGA
  TGAGGCTCTGAAACCTGAGGATGCCAAGCCCTGTGGAAGCCAGCCGTGTCCCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013906
• Insert Size: 2718 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013906.3, NP_038934.2
• RefSeq Size: 4613 bp
• RefSeq ORF: 2718 bp
• Locus ID: 30806
• Cytogenetics: 9 16.49 cM
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. This gene is expressed in mouse lung, heart and macrophage-rich areas of atherosclerotic plaques. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. This gene is located adjacent to a related ADAMTS gene on chromosome 9. [provided by RefSeq, May 2016]