Chaf1a (NM_013733) Mouse Untagged Clone

CAT#: MC222524

Chaf1a (untagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chaf1a
• Synonyms: AL023013; AL024058; Cac1p; CAF-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222524 representing NM_013733
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGAGGAGCCGGAGGCCGCGACGCGGACAGCCGCGGCCGTGGATTGCAAAGACAGACCCGGGTTCC
  CAGTGAAGAGGTTAATACAAGCCCGCTTGCCCTTCAAACGCCTGAACCTTGTTCCCAAGGAGAAAGTTGA
  GGAAGACACATCACCAAAAGCAGCTGTGGAAAGCAAAGTCCCGGATCTACAATTGTCTTTGGGCACTTTT
  GAAAGCCAGTGTCACACAGGCTCTCACGTAGGCTTGAGTACAAAGTTGGTCGGTGGCCAGGGCCCCATTG
  ATAGCTTCTTGAGGGCAACAATCAAGCCAGTACCGAGTGTGGTCATTATCGACCTGACGGAGAACTGCAG
  TGACATTCCAGACAGCCCTGAGGGCCACAGTGAACTAAGTCCTGACACTGCTGGTGTGGTCACCACAGTG
  GAAGGAGCTGCCAAGCAGCAGGAGCACTCTGCAGCAGAGCTGTGTCTCCTAGAGACCCCTTCAGACATCA
  CTTGTCACATGGAAGAGGAGCCTGGGAGTCCAGGAGACCCAAAGAGGACGGGTGACTGTCAGGCTGGTTC
  ACTACAGAGCTGTCCGGAGCTGACCCCAGGTTCTAGGACATGCCCCACCAAGGAGTTATCAAGCTGGAGC
  AAGGCAGGGGATCTCCTATTTATAGAGAAAGTGCCTGTGGTGGTGCTAGAGGACATCCTGGCCACTAAGC
  CCTCCATTGCCTCTCTTCCCATGATGTCCCTGGATAGGAGTGTCACTTCAGAGAGTGAAATACTGGAGTC
  CTGCCCCGAGGATGACTCCATACTGAGCCACTCCTCCACCAACTCCTCTTCTCCAACCAGCTCTCCTGAG
  GGACCATCCACACCCCCAGAACATCGGGGTGGGAGAAGCTCCCCCTCCACCCCAGCCTGCAGAGTTGCCA
  AGAACTTTGTCAAAGGCTCCACAGAGAAGGGCAGGAGCAAACTGCACAGAGATAGAGAACAGCAGAGGGA
  AGAGAAGGAAAAGCTGAGGGAAGAGATACGACGAGCCAAGGAGGAGGCACGGAAGAAGAAGGAGGAGGAG
  AAGGAGCTGAAGGAAAAGGAGCGGAGGGAAAAGCGGGAGAAAGATGAGAAGGAGAAGGCGGAGAAGCAGC
  GGCTAAAGGAGGAAAAACGCAAGGAGCGGCAGGAAGCTCTGGAGGCTAAACTGGAGGAGAAGAGGAAGAA
  GGAGGAAGAGAAGCGGTTACGGGAGGAAGAGAAGCGGTTACGGGAAGAAGAGAAGCGCATTAAGGCAGAA
  AAGGCAGAGATCACTCGATTCTTCCAGAAACCGAAGACTCCGCAGGCTCCCAAGACCCTGGCCGGTTCCT
  GTGGTAAGTTTGCCCCCTTTGAAATCAAAGAACACATGGTGCTGGCTCCACGGTGCCGAGCTGCCTTGGA
  TCAAGACCTGTGCGATCAGCTGGACCAGCTCCTGCAGCAGCAGAGTGTGGCAAGCACCTTCCTCAGTGAC
  CTAAAAAGCCGGCTGCCCTTGAGATCGGGGCCCACCCGGGTCTGTGGCCATGACACAGACATCATGAACA
  GGGATGTGGTCATTGTGGAGAGCAGCAAGGTGGACGGTGTGTCTGAAAGGAAGAAGTTTGGCCGCATGAA
  GCTTCTGCAGTTCTCAGAGAACCACCGGCCAGCATACTGGGGGACGTGGAACAAGAAGACAGCCATCATC
  CGCCCCCGGAACCCCTGGGCCCAGGACAAAGACCTTCTTGACTATGAGGTAGACAGTGACGATGAGTGGG
  AAGAGGAGGAACCTGGGGAGTCTTTGTCCCACAGTGAGGGGGATGAGGATGACGATGTGGGGGAAGATGA
  GGATGAGGACGATGGCTTCTTTGTGCCCCATGGGTACTTATCTGAAGATGAAGGCGTGACTGAGGAGTGT
  GCAGACCCTGAGAACCACAAAGTACACCAGAAGCTGAAGGCCAAGGAGTGGGATGAGCTCCTAGCCAAGG
  GCAAGCGCTTTCGTGTGCTGCAGCCTGTGCACGTGGGATGTGTATGGGCATCAGAGGCAGCCAACTGCAC
  GAGTTCCGACTTGAAGCTGCTGCAGCAGTTCACTGCATGCCTGCTGGATGTGGCATCCCCTGATGAGCCA
  GAACCCGGGGCCTCCAGGAGGGAAAAGAGGGACCAGCATATCCTGGCCCAGTTGCTTCCGCTGCTACATG
  GCAATGTGAATGGGAGCAAGGTGATCATCCATGAATTCCAGGAGCAATGCCGCAGGGGCCTGCTGACCCT
  TCCAAGTCCCACCCCACACCTGCAGATGCCCAACTTGGAGGATGCTGTTGCTGTGCCATCCAAGGCCAGG
  CTGAAGCGCCTTATCTCTGAGAACTCTGCCTATGAGAAGCGGCCCAACTTCCGTATGTGCTGGTATGTGC
  ACCCAGAGGTGCTCAAGAGCTTTGGCCAGGAGTGCCTGCCAGTGCCCTGCCAGTGGACCTACATCACCAC
  CATGCCCTCAGCACCCAGGGAAGACAGTGGCAGTGCTTCTACTGAGGGGCCTGGCCAGAGCACACCTATG
  CTACTCAAGCGGAAGCCAGCGGCCACCATGTGCATCACGCAGTTTATGAAGAAGCGGCGCTATGATGGAC
  AGGTTGGGTCTGGGGACATGGATGGCTTCCAGGCAGACACAGAAGAGGATGAAGAGGATGACACAGACTG
  TATGATCATAGATGTGCCAGATGTTGGGAGTGACGTGTCAGAAGCTCCCATACCTGCTCCCACACTTTGC
  AAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013733
• Insert Size: 2736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013733.3, NP_038761.1
• RefSeq Size: 3308 bp
• RefSeq ORF: 2736 bp
• Locus ID: 27221
• UniProt ID: Q9QWF0
• Cytogenetics: 17 D
• Gene Summary: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.[UniProtKB/Swiss-Prot Function]