Trpc3 (NM_019510) Mouse Untagged Clone

CAT#: MC222522

Trpc3 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 3 (Trpc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc3
• Synonyms: Mwk; Trcp3; Trp3; Trrp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_019510.2
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCAAGGTCAAGAAGTGCCGGGAGCCCGCGAGGGTGACCTTGCCCGCTCCGGAGGAGGAGGAGG
  ATGGCGAGGCCGAGGGAGGCGAGTCGCAGCGCCGCCGCCGGGGCTGGAGGGGCGTCAACGGCGGGCTGGA
  GCCGCCCTGCCCGCGCGCGCCGCCGTCCCCGGGGCCCGACGCGTCGTCGGAGGGCAGCCCATCCAGGTGG
  CGCACTGCGGGGATGCGTGACAAGGGCCGGCGCCAGGCAGTGCGTGGCCCGGCCTTCATGTTCGGTGCTC
  GTGGGCCCAGCCTCACAGCTGAGGAGGAGCGCTTCCTGGATGCTGCGGAGTACGGCAACATCCCTGTGGT
  GCGCAAGATGCTGGAGGAGTCTCGCACGCTCAATGTCAACTGCGTGGACTACATGGGCCAGAACGCACTG
  CAGCTGGCTGTAGGCAATGAGCATCTGGAGGTGACCGAGCTGCTGCTGAAGAAGGAGAACCTGGCGCGGA
  TCGGTGATGCGCTGCTGCTAGCCATCAGCAAGGGCTATGTGCGCATCGTGGAGGCCATCCTGGGCCACCC
  AGGCTTTGCAGCCAGCCGGCGCCTGACCCTCAGCCCTTGCGAGCAAGAACTGCGAGATGATGACTTCTAT
  GCTTACGACGAGGATGGCACACGCTTCTCGCCTGACATCACGCCCATCATCCTGGCTGCACACTGCCATA
  AGTACGAGGTGGTGCACCTGCTGCTACTCAAGGGTGCACGCATCGAGAGGCCACACGACTACTTCTGTCG
  CTGCTCTGACTGCGCTGAGAAACAAAGGCTTGACGCCTTCAGCCACTCAAGGTCTAGGATCAATGCCTAC
  AAGGGACTGGCCAGCCCAGCATACTTGTCGCTGTCCAGCGAGGACCCCGTGCTCACAGCACTGGAGCTCA
  GCAATGAGCTGGCCAAGCTGGCCAACATAGAGAAGGAGTTCAAGAATGACTACAGGAAACTCTCCATGCA
  ATGCAAAGACTTCGTAGTGGGTGTGCTGGACCTGTGTCGGGACTCGGAGGAGGTGGAAGCCATTCTGAAT
  GGAGATCTGGAATCAGCCGAGCCCCTGGAAAGACACGGGCACAAAGCGTCACTGAGTCGTGTCAAACTTG
  CCATTAAGTATGAAGTCAAAAAGTTTGTGGCTCACCCCAACTGCCAACAGCAGCTTTTGACAATCTGGTA
  TGAGAACCTTTCCGGCCTCCGGGAGCAGACCATCGCTATCAAGTGTCTGGTCGTGTTGGTCGTGGCCTTG
  GGTCTTCCATTCCTCGCCATCGGCTATTGGATTGCACCTTGTAGCAGGCTGGGGAAGATTCTTCGAAGCC
  CCTTCATGAAGTTTGTGGCCCACGCTGCCTCCTTCATCATCTTTCTGGGTCTGCTTGTATTCAACGCCTC
  GGACAGATTTGAAGGCATCACCACGCTGCCAAACATCACTGTTATCGACTACCCCAAGCAAATCTTCAGG
  GTGAAGACCACCCAGTTCACGTGGACGGAAATGTTAATTATGGTCTGGGTTCTTGGGATGATGTGGTCTG
  AGTGCAAGGAGCTGTGGCTGGAGGGGCCCCGGGAGTACATCGTGCAGTTGTGGAATGTGCTTGATTTCGG
  CATGCTCTCCATCTTCATCGCCGCGTTCACAGCCAGGTTCCTCGCTTTCCTGCAGGCCACGAAGGCGCAG
  CAGTATGTGGATAGTCACGTGCAGGAGAGCGATCTGAGCGAAGTCACGCTCCCACCTGAGGTTCAATATT
  TCACCTATGCTAGAGACAAATGGCTCCCCTCAGACCCTCAGATCATATCTGAAGGGCTCTATGCCATAGC
  TGTGGTGCTCAGCTTCTCCCGGATTGCGTACATCCTCCCTGCAAACGAGAGCTTTGGGCCCTTGCAGATC
  TCTCTTGGAAGGACTGTGAAGGACATATTCAAGTTCATGGTTCTCTTCATTATGGTGTTCCTGGCTTTCA
  TGATTGGCATGTTCATACTTTATTCTTACTACCTTGGGGCCAAAGTAAATCCTGCTTTTACCACGGTTGA
  AGAAAGTTTCAAGACTTTGTTTTGGTCCATATTTGGACTGTCTGAAGTGACTTCTGTTGTCCTCAAATAT
  GATCACAAATTCATAGAGAATATTGGCTATGTTCTTTATGGGATATATAATGTAACTATGGTGGTCGTTT
  TACTCAACATGCTAATTGCTATGATTAATAGCTCATACCAAGAGATCGAGGATGACAGTGATGTAGAATG
  GAAGTTTGCTCGTTCCAAACTTTGGCTCTCCTACTTCGATGATGGAAAAACATTACCTCCACCCTTCAGT
  CTGGTCCCTAGTCCAAAATCATTTGTTTACTTCATCATGAGGATCACTAACTTTTCCAAATGCAGGAGGA
  GAAGACTGCAGAAGGATCTGGAACTGGGCATGGGTAACTCAAAGTCCAGGTTAAACCTCTTCACACAGTC
  TAACTCGAGAGTTTTTGAATCACACAGTTTTAACAGCATTCTCAATCAGCCAACACGATATCAGCAGATA
  ATGAAAAGACTCATAAAACGGTATGTTTTGAAAGCACAAGTAGACAAAGAAAACGATGAGGTGAATGAAG
  GTGAACTGAAAGAAATCAAGCAGGATATCTCCAGCCTTCGTTATGAACTTTTAGAAGATAAGAGCCAAGC
  GACGGAGGAATTAGCCATCTTGATTCATAAACTCAGTGAGAAACTGAACCCCAGTGTGCTGAGATGTGAG
  TGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_019510
• Insert Size: 2733 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019510.2, NP_062383.2
• RefSeq Size: 3694 bp
• RefSeq ORF: 2733 bp
• Locus ID: 22065
• Cytogenetics: 3 17.93 cM