Ptchd4 (NM_028474) Mouse Untagged Clone

CAT#: MC222500

Ptchd4 (untagged) - Mouse RIKEN cDNA 3110082D06 gene (3110082D06Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptchd4
• Synonyms: 3110082D06Rik; Gm321
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222500 representing NM_028474
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACGGCCGGGAGCGCCTGCGAGCTGGATCTGGTGGAGGATGCTGCGGCAGGTGCTTCGCAGAGGGC
  TCCAGTCATTCTGCCACAGGCTGGGCTTATGCGTGAGCCGGCACCCGGTCTTTTTCCTCACCGTGCCGGC
  AGTCCTGACCATCACCTTCGGCCTCAGCGCGCTCAACCGCTTCCAGACCGAGGGCGACCTGGAGCGCCTG
  GTTGCTCCCAGCCACAGCCTGGCCAAGATCGAGCGCAGCCTAGCCAGCAGCCTTTTCCCCCTGGACCAGT
  CCAAAAGCCAGCTCTATTCGGACTTACACACCCCTGGGAGGTATGGCAGGGTGATCCTCCTCTCCTCACC
  CGGGGACAATATTTTGCTCCAGGCTGAGGGGATTCTGCAGACCCACCGAGCCGTGATGGAAATGAAGGTG
  AACCACAAGGGCTATAATTATACTTTTTCCCATCTGTGTGTGTTGAGAAATCAGGATAAGAAATGCGTGC
  TGGATGATATTATTTCAGTGCTAGAGGATCTCAGGCAGGCTGCCGTCTCCAATAAGACAACAGCCAGGGT
  GCAAGTAAGGTATCCCAACACTAAATTAAAGGATGGGAGGAACAGTTTTATTGGACACCAGCTGGGCGGG
  GTAGTGGAAGTGCCAAACAGCAAAGACCAGCGGGTCAAGTCAGCCAGAGCCATTCAAATCACCTACTACC
  TCCAGACCTATGGTTCTGCCACACAAGACCTCATAGGGGAGAAGTGGGAGAATGAGTTCTGTAAGCTTAT
  GAGGAAGCTCCAGGAGGAGCATCAAGACCTCCAACTGTACTCTCTGGCATCTTTTAGCCTCTGGAGAGAC
  TTTCATAAGACCAGCATCCTGACCAGAAGCAAGGTCCTGGTGAGCCTAGTGCTGATCCTGACCACGGCCA
  CCCTGTCCAGCTCCATGAAGGACTGCTTGCGCAGTAAGCCCTTCCTGGGCCTCCTGGGGGTGCTCACGGT
  CTGCATTTCCATCGCCACTGCAGCCGGGATATTCTTCATCACTGATGGGAAGTACAACTCAACCCTGCTG
  GGAATCCCTTTCTTCGCCATGGGTCATGGAACTAAAGGAGTATTTGAGCTTCTGTCTGGATGGCGGAGAA
  CCAAAGAGAATTTACCCTTCAAAGACAGGGTAGCAGATGCCTACTCTGATGTGATGGTCACCTACACCAT
  GACCAGTTCCCTGTACTTCATCACATTTGGTATGGGTGCCAGCCCCTTCACAAACATAGAGGCCGTGAAG
  ATCTTCTGTCAGAATATGTGTGTCTCTATTCTGTTGAACTACTTCTACATTTTCTCTTTCTTTGGCTCTT
  GCCTGGTCTTTGCTGGACAACTTGAGCAAAATCGATACCACAGCATCTTTTGCTGTAAGATCCCTTCTGC
  AGAATACCTGGACCGCAAACCTGTGTGGTTCCAGACAGTAATGAGTGATGGGCACCAACAGACATCGCAT
  CATGAGACAAACCCCTACCAGCACCATTTCATTCAGCACTTCCTTCGAGAACATTACAATGAGTGGATTA
  CCAATATCTATGTGAAGCCATTTGTGGTCATCCTGTATCTCATTTACGCCTCTTTCTCCTTCATGGGGTG
  CTTGCAGATCAGTGATGGAGCCAGCATCATCAACCTACTAGCCAGTGATTCCCCAAGTGTTTCCTACGCC
  ATGGTTCAGCAAAAATATTTCAGCAACTATAGCCCTGTAATAGGATTCTACGTCTATGAACCCCTGGAGT
  ACTGGAACAGCAGTGTCCAAGAGGATCTGCAGAGGCTCTGTAGTGGGTTCACTGCAGTGTCCTGGGTAGA
  GCAGTATTACCAGTTTCTGAAAACGAGCAACATCAGTGCCAATAACAAGACTGACTTCATCAGTGTCCTG
  CAAAGCTCGTTTTTAAAAAAGCCAGAATTCCAACACTTTCGGAATGATATCATTTTCTCCAGAGCTGGTG
  ATGAAAACAACATCATTGCTTCTCGCTTGTATCTGGTGGCCAGGACTAGCAGGGACAAGCAGAAGGAAGT
  CATAGAAGTTCTGGACAAGTTGAGGCCCCTGTCTCTCTCAAAGAGTATCCGGTTCATCGTGTTTAATCCT
  TCCTTTGTTTTCACAGACCATTACAGTTTGTCAGTCACTGTGCCTGTTCTGATCGCAGGCTTTGGGGTTC
  TCCTGGTGTTAATCCTGACTTTCTTCCTAGTGATCCATCCTCTGGGAAACTTCTGGCTAATTCTTAGCGT
  CACCTCAATTGAGCTGGGCGTTCTGGGCTTAATGACGTTGTGGAATGTCGACATGGATTGCATTTCTATC
  TTGTGCCTTATCTACACTTTAAATTTTGCCATTGACCACTGTGCACCACTGCTTTATACATTTGTATTAG
  CAACTGAGCACACCAGAACACAGTGTATAAAAAGCTCCCTGCAAGAGCATGGGACAGCCATTTTGCAAAA
  CATTACTTCTTTTCTTATTGGGTTGCTCCCCCTCCTCTTTGTGCCTTCAAACCTGACCTTCACACTGTTC
  AAATGCTTGCTGCTGACCGGGGGTTGCACGCTTCTGCACTGTTTTGTTATCTTACCTGTGTTCCTAACCT
  TTTTTCCTCCTTCCAAAAAGCACCACAAGAAAAAGAAACGGGCCAAACGAAAGGAGAGAGAGGAAATTGA
  ATGCATAGAAATCCAAGAGAACCCTGATCACGTCACCACAGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028474
• Insert Size: 2706 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028474.1, NP_082750.1
• RefSeq Size: 6890 bp
• RefSeq ORF: 2706 bp
• Locus ID: 627626
• Cytogenetics: 17 B3
• Gene Summary: Could act as a repressor of canonical hedgehog signaling by antagonizing the effects of SMO, as suggested by down-regulation of hedgehog target genes, including GLI1, PTCH1, and PTCH2 in PTCHD4-expressing cells.[UniProtKB/Swiss-Prot Function]