Unc5a (NM_153131) Mouse Untagged Clone

CAT#: MC222478

Unc5a (untagged) - Mouse unc-5 homolog A (C. elegans) (Unc5a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unc5a
• Synonyms: mKIAA1976; Unc5h1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222478 representing NM_153131
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCACTGCCTGGCTTCGTGGTTCGG
  GTGCCCAGCAGAGTGCCACAGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTGCCCCACTTCCT
  GGTAGAGCCGGAGGACGTGTACATTGTCAAGAACAAGCCCGTGCTGCTGGTGTGCAAGGCTGTGCCCGCC
  ACCCAGATCTTCTTCAAGTGCAACGGGGAATGGGTTCGCCAGGTCGATCACGTCATTGAACGCAGCACTG
  ACGGCAGCAGCGGATTGCCAACCATGGAGGTCCGGATCAACGTATCAAGGCAGCAGGTCGAGAAAGTGTT
  TGGGCTGGAGGAGTACTGGTGCCAGTGTGTGGCATGGAGCTCCTCAGGAACCACCAAAAGCCAGAAGGCC
  TACATCCGGATTGCCTATTTGCGCAAGAACTTTGAGCAGGAGCCGCTGGCCAAGGAAGTGTCACTGGAGC
  AAGGCATTGTGCTACCTTGTCGCCCCCCGGAAGGAATCCCCCCAGCTGAGGTGGAGTGGCTCCGAAATGA
  GGACCTCGTGGACCCCTCCCTCGACCCCAATGTGTACATCACACGGGAGCACAGCCTAGTCGTGCGGCAG
  GCCCGCCTGGCCGACACTGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCCCGTCGCCGAAGCGCCT
  CTGCGGCCGTCATTGTTTATGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGTGCCAG
  CTGTGGGCGTGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCACCTCTCAACGGGGGCGCCTTC
  TGTGAGGGGCAGAATGTCCAGAAAACAGCCTGCGCCACTCTGTGCCCAGTGGATGGGAGCTGGAGCCCAT
  GGAGTAAGTGGTCAGCCTGCGGGCTTGACTGCACCCACTGGCGGAGCCGGGAGTGCTCCGACCCAGCGCC
  CCGCAACGGAGGTGAGGAGTGCCGGGGTGCTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGCCTG
  CACACCTCTTCCGGCCCCGAGGACGTGGCTCTCTACATCGGCCTCGTCGCCGTGGCCGTGTGCCTCATCT
  TGCTGCTGCTGGTCCTCGTCCTCATCTACTGCCGCAAGAAGGAAGGACTGGACTCAGACGTGGCTGACTC
  ATCCATCCTTACCTCAGGCTTCCAGCCTGTCAGCATCAAGCCCAGCAAAGCAGACAATCCCCATCTGCTC
  ACCATCCAACCGGACCTCAGCACCACCACGACCACCTACCAGGGCAGCCTGTGTCCCCGGCAGGATGGAC
  CCAGCCCCAAGTTCCAGCTCTCTAATGGTCACCTGCTCAGCCCACTGGGCAGTGGCCGCCATACGCTGCA
  CCACAGCTCCCCCACCTCTGAGGCTGAGGACTTCGTCTCCCGCCTCTCCACCCAAAACTACTTTCGTTCT
  CTGCCCCGCGGTACCAGCAACATGGCCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTA
  ACACAGGAATCAGCCTCCTCATACCCCCGGACGCCATCCCCCGAGGAAAGATCTACGAGATCTACCTCAC
  TCTGCACAAGCCAGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCTATCGTTAGC
  TGTGGGCCCCCAGGAGTCCTGCTCACCCGGCCAGTCATCCTTGCCATGGACCACTGCGGGGAGCCCAGTC
  CCGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCCTGTGAGGGCAGCTGGGAGGACGTGCTGCACCTTGG
  TGAGGAGTCGCCCTCTCATCTCTACTACTGCCAGCTGGAGGCCGGGGCCTGCTATGTCTTCACCGAGCAG
  CTAGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCACCAAGCGCCTCAGGCTCCTTCTGT
  TTGCCCCTGTGGCCTGTACGTCCCTCGAGTACAACATCCGAGTGTACTGCCTGCACGACACCCACGATGC
  TCTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGTGGACAGCTGATCCAGGAGCCCCGTGTCCTGCAC
  TTCAAAGACAGTTACCACAACCTACGTCTGTCCATCCACGACGTGCCCAGCTCCCTGTGGAAGAGCAAGC
  TCCTTGTCAGCTACCAGGAGATCCCTTTTTACCACATCTGGAATGGCACTCAGCAGTATCTGCACTGCAC
  CTTCACCCTGGAGCGCGTCAATGCCAGCACCAGCGACCTGGCCTGCAAGGTGTGGGTGTGGCAGGTGGAG
  GGAGATGGACAGAGCTTCAACATCAACTTTAACATCACTAAGGACACGAGGTTTGCTGAAATGCTGGCTC
  TGGAGAGTGAAGGGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCA
  AAAGATCATTACCAGCCTGGACCCACCCTGCAGCCGGGGCGCCGACTGGCGAACTCTAGCCCAGAAACTT
  CACCTGGACAGCCATCTTAGCTTCTTTGCCTCCAAGCCCAGCCCTACAGCCATGATCCTCAACCTATGGG
  AGGCGCGGCACTTCCCCAACGGCAACCTCGGCCAGCTGGCCGCAGCTGTGGCCGGACTGGGCCAGCCAGA
  TGCTGGCCTCTTCACCGTGTCAGAGGCCGAGTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153131
• Insert Size: 2697 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153131.3, NP_694771.1
• RefSeq Size: 3974 bp
• RefSeq ORF: 2697 bp
• Locus ID: 107448
• UniProt ID: Q8K1S4
• Cytogenetics: 13 B1
• Gene Summary: Receptor for netrin required for axon guidance. Functions in the netrin signaling pathway and promotes neurite outgrowth in response to NTN1. Mediates axon repulsion of neuronal growth cones in the developing nervous system in response to netrin. Axon repulsion in growth cones may be mediated by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.