Pard3 (NM_001013581) Mouse Untagged Clone

CAT#: MC222474

Pard3 (untagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pard3
• Synonyms: AA960621; AI256638; Asip; D8Ertd580e; Par-3; Par3; Pard-3; Pard3a; Phip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222474 representing NM_001013581
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTTCATGTCCGCCGGAGCAGCGACCCAGCTTTAACTGGCCTTTCCACTTCTGTCAGTGATAACA
  ACTTTTCCTCAGAGGAGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCA
  GAACACCGCTGGAAGTCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTTCCACGGGAT
  CCCAGTAGCTGGTCCAACCAGTTCCAGCGAGACAACGCCCGCTCCTCCCTGAGCGCCAGCCACCCAATGG
  TAGACCGGTGGCTGGAGAAGCAAGAACAGGATGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCC
  GGTTGGACATGCTGATACCGGATTGGAGAACATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTA
  CAAGTCCCCAACGATGGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACAT
  TGGGGTTGTTAGTGAAGCGGTTGGAGAAAGGCGGTAAGGCTGAGCAAGAAAACCTTTTCCATGAGAATGA
  CTGCATTGTGAGGATTAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAACATATGTTCCGC
  CAAGCTATGCGTGCGCGTGTCATTTGGTTCCATGTGGTCCCTGCAGCAAACAAGGAGCAATATGAACAAC
  TGTCCCAACGCGAGAAGAACAACTACTCCCCAGGCCGCTTCAGCCCTGACAGCCACTGTGTGGCCAACAG
  GAGTGTGGCCAACAATGCCCCTCAAGCATTGCCCAGAGCACCCAGACTGAGTCAGCCACCCGAGCAGCTG
  GATGCTCACCCCCGACTACCTCATAGTGCTCACGCCTCAACCAAACCACCCGCAGCCCCGGCCTTGGCTC
  CACCCAGTGTGCTTAGTACCAACGTAGGCAGTGTGTACAACACGAAGAAAGTAGGCAAGAGGCTCAACAT
  CCAGCTTAAGAAAGGTACAGAAGGACTGGGATTCAGCATCACCTCCCGGGACGTCACCATAGGTGGCTCA
  GCTCCCATTTATGTCAAGAATATCCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGACTCAAGGCAGGAG
  ACCGGCTAATAGAGGTCAATGGAGTAGATTTAGCAGGCAAATCCCAGGAGGAAGTTGTTTCCCTGTTGAG
  AAGCACCAAGATGGAGGGGACTGTGAGCCTTCTGGTCTTTCGTCAGGAAGAGGCTTTCCACCCAAGGGAA
  ATGAATGCTGAACCAAGCCAGATGCAGACTCCAAAAGAAACGAAAGCTGAAGATGAGGACGTTGTTCTCA
  CACCCGATGGTACCAGGGAGTTTCTGACTTTTGAAGTTCCACTGAATGACTCAGGATCTGCAGGGCTTGG
  TGTCAGTGTCAAGGGGAACCGTTCCAAAGAGAACCACGCAGATTTGGGGATCTTCGTTAAATCCATTATC
  AATGGTGGAGCTGCATCTAAAGATGGAAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAATGGAGAAT
  CTCTACTGGGCAAAGCCAACCAGGAAGCCATGGAGACTCTACGGAGGTCCATGTCCACCGAGGGCAACAA
  GCGTGGCATGATCCAGCTCATTGTGGCGAGGCGGATCAGCAGATGTAACGAGCTGCGGTCTCCTGGGAGC
  CCTGCTGCACCTGAGCTGCCCATCGAGACAGAACTGGATGACCGAGAACGCAGGATCTCACACTCCCTCT
  ACAGTGGGATCGAGGGGCTGGATGAGTCGCCCACCAGGAACGCAGCACTGAGCAGGATAATGGGTAAATG
  CCAGCTGTCCCCCACGGTGAACATGCCTCACGATGACACTGTCATGATTGAAGATGACAGGCTGCCTGTG
  CTCCCTCCTCACCTCTCTGACCAGTCCTCCTCCAGCTCCCATGATGACGTTGGATTCATAATGACAGAAG
  CAGGCACGTGGGCCAAAGCTACCATCAGTGACTCAGCCGACTGCTCATTGAGTCCAGATGTTGATCCGGT
  TCTCGCTTTTCAACGGGAAGGATTTGGACGCCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAGAT
  GCCAGTCAATTGGATTTCGTTAAAACACGAAAATCAAAAAGCATGGATTTAGGTATAGCTGACGAGACTA
  AACTCAATACAGTGGATGACCAGAGAGCAGGCTCTCCCAGTAGAGATGTGGGACCCTCCTTAGGTCTGAA
  GAAATCTAGCTCCTTAGAAAGTCTGCAGACCGCTGTCGCCGAGGTGACGCTGAACGGGAACATTCCTTTC
  CACCGCCCTCGGCCACGAATCATCCGGGGAAGGGGCTGCAACGAAAGCTTCAGAGCCGCCATTGACAAGT
  CCTACGACAAGCCCATGGTTGATGATGACGACGAAGGCATGGAGACCTTGGAAGAAGACACAGAGGAAAG
  CTCGAGGTCAGGGAGGGAGTCCGTGTCCACGTCCAGCGATCAGCCTTCCTATTCTCTGGAGAGGCAAATG
  AATGGAGACCCAGAGAAAAGGGACAAGACAGAGAGGAAAAAGGACAAAGCCGGAAAGGATAAGAAGAAAG
  ACCGAGAGAAGGAGAAGGATAAACTGAAAGCCAAGAAGGGGATGCTGAAAGGCTTGGGGGACATGTTCAG
  CCTGGCCAAACTGAAGCCGGAGAAGAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013581
• Insert Size: 2691 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013581.2, NP_001013599.1
• RefSeq Size: 3233 bp
• RefSeq ORF: 2691 bp
• Locus ID: 93742
• UniProt ID: Q99NH2
• Cytogenetics: 8 74.66 cM
• Gene Summary: Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1, also called PAR-3o1) differs in the 5' and 3' UTR and has multiple coding region differences compared to variant 5. These differences cause translation initiation at a downstream AUG and an isoform (1) with a shorter N-terminus and shorter and distinct C-terminus compared to isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.