Dnajc6 (NM_001164585) Mouse Untagged Clone

CAT#: MC222471

Dnajc6 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 6 (Dnajc6), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnajc6
• Synonyms: 2810027M23Rik; mKIAA0473
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222471 representing NM_001164585
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCAGCTATGGGGGAGGCCTTTTTGACATGGTGAAAGGCGGTGCAGGGAGGCTATTCAGTAACC
  TGAAGGACAACTTGAAAGACACTCTCAAAGATACATCCTCCAGGGTCATCCAGTCTGTTAGCAGCTACAC
  AAAGGGAGACCTAGACTTTACTTACGTCACCTCCAGGATTATCGTGATGTCCTTTCCTGTGGATAGTGTT
  GACATAGGATTCAGGAATCAGGTTGATGACATCCGTAGCTTTTTGGATTCCAGACATCTTGACCACTACA
  CAGTATACAACTTGTCACCCAAGTCTTACCGAACTGCCAAGTTCCACAGCCGGGTCTCAGAGTGCAGTTG
  GCCCATCAGGCAGGCTCCAAGTCTACACAACCTGTTTGCTGTGTGTAGGAATATGTATAACTGGCTACTG
  CAGAACCCCAAAAATGTCTGTGTTGTCCACTGCTTGGATGGACGGGCCGCGTCGTCAATCCTGGTTGGTG
  CTATGTTCATTTTCTGTAATCTCTACTCCACGCCTGGCCCTGCTGTTCGATTACTCTACGCAAAGCGACC
  AGGAATTGGACTGTCTCCGTCTCACAGGAGGTACTTAGGCTACATGTGCGACCTACTTGCAGATAAGCCC
  TACCGCCCTCACTTCAAGCCACTCACGATCAAGGCAATCACTGTCAGCCCAGTTCCCTTCTTCAATAAGC
  AGAGAAATGGGTGCCGCCCTTACTGTGACGTTCTGATTGGAGAAACTAAAATATATTCAACGTGCACAGA
  CTTTGAACGAATGAAAGAATACCGTGTCCAAGATGGGAAAATCTTCATCCCTCTGAACATCACAGTCCAA
  GGTGATGTCATCGTTTCCATGTACCACTTGCGGTCAACCATCGGGAGCCGGCTGCAGGCGAAGGTGACCA
  ACACACAGATATTCCAGCTCCAGTTTCATTCTGGGTTTATACCACTGGACACGACAGTTTTAAAGTTCAC
  CAAGCCTGAGCTGGATGCATGTGATGTACCAGAAAAATATCCTCAGCTGTTCCAAGTGACACTGGATATA
  GAAGTCCAGCCCCAAGACAAAGTGATCGACTTAACTCCACCATGGGAACACTACTGCACAAAAGATGTGA
  ACCCCAGCATCCTCTTCTCCTCTCAGCAGGAGCATCAGGACACTCTGGCCTTAGGGGGTCAGGCTCCAGC
  CGATCTCCCACCAGACCATCCCAGGAATCTTGGGCAAGGAGGCTTCTTTGCCTCTCTCTGTTGGCAAGAT
  CAGAAATCAGAGAAGTCACGCTGTGAGGAGGACCACGCTGCCCTGGTGAATCAGGAAAGTGAGCAGTCAG
  ATGATGAGCTACTGACTCTCTCCAGCCCCCATGGCAATGCAGAGGGTGATAAGCCTCATGGAGCCAAGAA
  GCCAGGCAAAAAGCAGCAGGAGCCAGCTGCGCCTCCTCCCCCAGAGGAAGTGGACCTCCTGGGCCTGGAA
  GGGTCTGATGTGAGCACGAACTTCTCTTCCTTGGCAGCTCCTCCCAGCAATTCTGAGTTGCTGAGTGACC
  TGTTTGGGGGTGTAGGTGCCACAGGTCCTGCTCAGGCAGGACAGGCTGGGGTAGAAGATGTGTTTCATCC
  TAGTGGACCTGTGTCTGCACAGTCCACACCGCGCCGAACCGCCACCTCCGCCTCTGCATCGCCAACCCTA
  AGAGTGGGAGAAGGTGCCACCTTTGACCCTTTTGGAGCCCCTGCTAAGCCACCAGGTCAGGATTTGTTGG
  GCTCCTTTCTAAACACATCCAGTGCTTCTAGCGACCCCTTTCTGCAGCCCACGAGAAGCCCTTCCCCTAC
  AGTGCATGCTTCTAGTACTCCTGCTGTGAACATCCAGCCCGACATTGCTGGAGGTTGGGACTGGCACACC
  AAACCAGGAGGTTTTGGAATGGGGAGCAAATCCGCTGCCACCAGCCCGACAGGCTCATCACATGGCACCC
  CCACCCATCAGAGCAAGCCCCAGACTCTGGATCCCTTCGCTGACCTGGGGACACTAGGTAGCTCTTCATT
  TGCCAGCAAACCTACCACCCCAACTGGATTGGGTGGAGGATTTCCACCTCTCAGCTCACCACAGAAAGCA
  TCCCCCCAGCCAATGGGTGGAGGGTGGCAGCAGCCAGCCGGCTACAACTGGCAGCAGACACAGTCTAAGC
  CACAGTCCAGTATGCCTCACTCCTCCCCTCAGAACCGACCCAATTACAACGTGAGCTTCTCGGCTATGCC
  TGCAGGGCAGAGCGAACGTGGAAAAGGATCTACTAACTTGGAAGGGAAACAAAAGGCTGCTGACTTTGAA
  GATCTGCTTTCTAGTCAAGGCTTCAATGCACACAAAGACAAAAAGGGGCCTCGAACAATAGCTGAGATGA
  GAAAGGAGGAGATGGCTAAGGAAATGGATCCTGAAAAACTAAAGATCCTGGAGTGGATAGAGGGCAAAGA
  GAGAAACATCAGAGCTCTGCTTTCCACAATGCACACCGTGCTGTGGGCTGGGGAGACCAAATGGAAACCC
  GTGGGCATGGCGGATCTGGTGACGCCGGAGCAAGTGAAGAAGGTGTACAGGAGGGCTGTGCTAGTGGTGC
  ACCCTGACAAGGCAACTGGGCAACCCTATGAACAATACGCAAAGATGATTTTCATGGAGCTAAATGATGC
  CTGGTCTGAATTTGAAAACCAAGGCCAAAAGCCCTTGTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164585
• Insert Size: 2703 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164585.1, NP_001158057.1
• RefSeq Size: 5230 bp
• RefSeq ORF: 2703 bp
• Locus ID: 72685
• UniProt ID: Q80TZ3
• Cytogenetics: 4 C6
• Gene Summary: Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream translational start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Variants 3. 4 and 6 encode the same isoform.