Chd1l (NM_026539) Mouse Untagged Clone

CAT#: MC222468

Chd1l (untagged) - Mouse chromodomain helicase DNA binding protein 1-like (Chd1l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chd1l
• Synonyms: 4432404A22Rik; Alc1; Snf2p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222468 representing NM_026539
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGGCTTGCCGCGCTTCCTGCAGGCGCTGCCGGCCGAACACGGGCCGGAGCCGCTTCGGACGC
  GGGTGCAGGAACCGGACTTACAGCAGTGGGGCTTGACTGGGATTCGTCTACGTTCTTACCAACTGGAAGG
  AGTCAATTGGCTAGTCCAGTGCTTCCATTGCCAGAATGGCTGCATCCTGGGAGATGAGATGGGCTTGGGG
  AAGACCTGCCAGACTATCGCTCTCCTCATTTACTTGGTGGGGAGACTGAATGATGAAGGGCCGTTTCTGG
  TTCTCTGTCCCTTGTCTGTTTTGAGCAACTGGAAAGAAGAGATGGAGAGATTTGCTCCAGGTCTTTCCTG
  TGTGACATACACAGGTGACAAGGAGGAGAGAGCCCGCCTTCAGCAGGACCTAAGACAGGAGTCAGGTTTT
  CACGTGCTGCTGACGACATACGAGATTTGCTTGAAAGATGCCTCATTTCTAAAATCCTTCTCTTGGAGCG
  TTCTTGCTGTGGATGAAGCTCACAGATTGAAAAACCAGAGCTCCCTGCTGCACAGGACACTTTCAGAGTT
  CTCAGCGGTCTTCCGTCTCCTGCTCACCGGAACGCCCATCCAGAACAGCCTCCGGGAGCTCTACTCCCTC
  CTCTGTGTCGTGGAGCCTGACCTCTTCTGCAGAGAACAGGTGGAAGATTTCGTTCAGCGTTACCAGGACA
  TCGAGAAAGAGTCCAAATCAGCAAGCGAGCTGCACAGGCTCTTGCAGCCATTTCTCCTGAGGCGTGTGAA
  AGCTCAGGTAGCCACAGAGCTCCCCAAGAAGACAGAGGTGGTGGTGTACCACGGCATGTCCGCTCTGCAG
  AAAAAGTACTACAAGGCCATCCTGATGAAAGACCTGGATGCATTTGAGAACGAGACAGCCAAGAAAGTCA
  AACTCCAGAACATCCTGACGCAGCTTCGGAAGTGTGTGGACCACCCGTATCTGTTCGATGGTGTGGAACC
  AGAGCCCTTTGAAGTTGGAGAGCACCTGATTGAGGCCAGTGGGAAGCTTCACCTGCTGGACAGGCTGCTG
  GCGTTTCTGTATTCTGGGGGCCATCGGGTTTTACTTTTCTCCCAGATGACACACATGCTGGATATTCTGC
  AGGACTATATGGACTACAGAGGCTACAGCTACGAGCGTGTGGATGGCTCCGTGAGAGGAGAGGAGAGACA
  CCTGGCCATTAAGAACTTTGGAAACCAGCCCATTTTTGTTTTTCTCCTGAGCACCCGGGCAGGTGGAGTG
  GGCATGAACTTAACAGCGGCAGACACCGTCATTTTTGTCGACAGCGACTTCAACCCTCAGAATGACTTGC
  AGGCGGCCGCCAGAGCTCACCGAATTGGCCAGAACAAGTCTGTTAAAGTCATCCGGCTGATCGGCCGCGA
  CACCGTGGAAGAAATCGTGTATAGGAAGGCGGCCTCCAAACTGCAGCTTACCAACATGGTCATAGAAGGA
  GGTCATTTCACACCAGGAGCCCAGAAACCTTCAGCCGAGGCCGACTTCCAGTTAAGTGAGATTCTCAAAT
  TCGGCTTGGATAAACTGCTGTCCTCTGAGGGGAGCTCCATGGAGGATATAGACCTGAAGTCCATCCTGGG
  AGAAACCAAAGATGGCCAGTGGACCCCTGATGCTCTGCCCGCAGCAGCAGCAGCAGGAGGAGGAAGCCTA
  GAACCAGAGGAAGGAAGTGAATTGGAATCCAGGTCTTATGAAAACCACATGTACTTGTTTGAAGGTAGAG
  ACTATTCTAAGGAGCCCAGTAAGGAAGACAGAAAGTCATTTGAGCAGCTGGTGAATCTTCAGAAAACCCT
  TCTAGAGAAGACAAGCCATGGGGGCCGGACCCTCCGGAACAAAGGCAGCGTTCTTATCCCAGGCCTTGCA
  GAAGGGCCCATCAAAAGGAAGAAGATCCTTAGCCCAGAGGAGCTGGAGGACAGACGGAAGAAAAGACAGG
  AAGCAGCGGCCAAGAGGAAGAGACTGATGGAGGAGAAGAGGAAGGAGAAGGAGGAGGCTGAACACAGGAA
  GAAGATGGCCTGGTGGGAATCCAACGGTTACCAGTCCTTCTGCCTGTCCTCGGAGGACAGCGAGCTTGAG
  GACCTGGAGGGCGGAGATGAGTCTTCTGCCGAGCTGGCTTATGAAGATCTAGACTCAACCTCCATTAACT
  ATGTTAGTGGCGACGTCACCCACCCCCAGGCTGGCGAAGAAGATGCTGTCATTGTGCACTGTGTAGATGA
  TTCTGGCCGCTGGGGCAGAGGTGGCTTATTCACAGCTCTGGAAGTACGATCAGCTGAACCAAGAAAAATA
  TACGAGCTGGCTGGGAAAATGGAAGACCTGAGTTTGGGTGATGTCCTTCTATTTCCTATCGACGATAAAG
  AGTCACGAGACAAGGGGCAAGATCTGTTGGCCTTGGTTGTGGCTCAGCACCGTGATCGCACCAATGTCCT
  GTCTGGCATTAAGATGGCAGCCCTAGAAGAGGGCCTGAAGAAGATATTTTTAGCAGCAAAGAAAAAGAAA
  GCAAGCGTTCATCTTCCACGAATTGGACATGCTACAAAGGGTTTTAACTGGTATGGTACCGAACGACTTA
  TCCGGAAACATTTGGCTACACGAGGCATCCCAACTTACATATACTACTTTCCCAGAAGTAAGGCCCGCCA
  TTCACAGCCTGCATCCTCCTCCTCAGCACCACTGGTTCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026539
• Insert Size: 2703 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026539.2, NP_080815.1
• RefSeq Size: 2989 bp
• RefSeq ORF: 2703 bp
• Locus ID: 68058
• UniProt ID: Q9CXF7
• Cytogenetics: 3 F2.2
• Gene Summary: DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding (By similarity).[UniProtKB/Swiss-Prot Function]