Osbpl8 (NM_175489) Mouse Untagged Clone

CAT#: MC222445

Osbpl8 (untagged) - Mouse oxysterol binding protein-like 8 (Osbpl8), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Osbpl8
• Synonyms: AA536976; AA536995; C730029P18Rik; D330025H14Rik; OR; ORP-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222445 representing NM_175489
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCAGCCTTAGCAGACGGAGAACCTGACAGATCTTCGCTTCTTGGTGATAGCAAAGATGTCCTTG
  GGCCATCAACTGTTGTAGCGAACAGTGACGAACCTCAGCATTTGACCCCAGGAAAGATGAGTCAGCGCCA
  AGGCAGAGACGCTAACCCAACCCCAACTAGAGATCTGCCTCAGCCTTCTCTCAGCCCCGCCAGCCTTCAC
  AGCCAGGGTTTTGAAAGAGGAAAAGAAGACATTTCTCAAAATAAAGATGACTCTTCACTTTCAATGTCAA
  AGAGCAAGTCTGAATCTAAACTATATAATGGCTCAGAAAAGGACAGTTCAACTTCAAGTAAACTCACGAA
  GAAAGAGTCTCTTAAGGTACAAAAGAAAAACTACCGTGAAGAGAAGAAGAGAGCCACGAAGGAGTTACTC
  AGCACCATCACAGACCCCTCTGTCATTGTCATGGCCGACTGGCTGAAGATTCGTGGTACCCTGAAGAGCT
  GGACCAAGCTGTGGTGTGTGTTGAAACCTGGCGTGCTTCTGATCTATAAAACACAAAAAAATGGTCAGTG
  GGTCGGAACAGTCCTTCTGAATGCTTGTGAGATCATTGAACGTCCATCAAAAAAGGATGGCTTTTGTTTC
  AAGCTTTTCCATCCTTTGGAGCAGTCTATTTGGGCAGTGAAGGGTCCTAAAGGTGAAGCGGTTGGCTCCA
  TAACCCAGCCTCTACCTAGCAGCTACTTGATTATCCGAGCTACTTCAGAGTCAGATGGAAGGTGCTGGAT
  GGATGCTTTGGAGTTGGCTTTGAAGTGTTCCAGTCTCCTTAAGCGGACCATGGTGAGGGAGGGGAAGGAG
  CACGACCTGAGCATCTCCTCAGACAGCACACACGTGACGCTGTATGGCTTACTGCGCGCTAACAATCTCC
  ACAGTGGCGACAACTTCCAGTTGAATGACAGTGAGATCGAAAGACAGCACTTTAAGGACCAAGATTTGTA
  CTCTGATAAATCTGACAAGGAGAACGACCCGGAGCACGATGAGTCTGACAACGAGGTGCTGGGGAAGAGC
  GAGGAGAGCGACACAGACACGTCCGAAAGGCAAGACGACTCGTACATCGACCCTGAGCCTGTGGAGCCCT
  TAAAGGAGACCACCTACATGGAGCAGAGCCACGAAGAACTGGGAGAGGCAGGTGAGGCCTCTCAAACGGA
  AACTGTGTCTGAAGAAAACAAAAGCCTTATCTGGACGCTGCTCAAGCAAGTCCGTCCCGGCATGGACCTA
  TCCAGGGTGGTTCTTCCTACGTTTATTTTGGAGCCCCGCTCTTTCCTGGATAAGCTTTCAGATTACTACT
  ACCACGCCGATTTCCTGTCTGAAGCTGCTCTAGAAGAAAATCCGTATTTCCGTTTGAAGAAAGTCGTGAA
  ATGGTATTTGTCAGGATTTTATAAGAAGCCAAAGGGACTAAAGAAACCATATAATCCTATACTTGGTGAG
  ACTTTCCGATGTTTATGGATTCATCCCAGAACAAACAGCAAAACTTTCTATATTGCTGAACAGGTGTCCC
  ATCACCCACCAATATCTGCCTTTTATGTCAGTAACCGGAAAGATGGGTTTTGCCTTAGTGGTAGCATCCT
  GGCTAAGTCCAAGTTTTATGGAAACTCGTTATCTGCCATATTGGAGGGAGAAGCCCGGTTAACTTTCTTG
  AACAGAGGTGAAGATTACGTCATGACAATGCCATATGCTCACTGTAAAGGAATTCTTTATGGCACAATGA
  CCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCGATACTTGAATTTAA
  ACTAAAGCCATTTCTAGGGAGTAGTGATTATGTTAACCAAATATCAGGGAAACTTAAATTGGGAAAAGAA
  GTCCTAGCTACTTTGGAAGGCCATTGGGATAGTGAAGTTTTCATTAATGACAAAAAGACTGATAACTCAG
  AGATTTTCTGGAATCCAACTCCTGACATTAAACAGTGGAGATTAATAAGGCACACTGTAAAGTTTGAAGA
  ACAGGATGATTTCGAATCAGAGAAACTCTGGCAACGAGTGACAAAAGCTATAAATGCCAAAGACCAAACG
  GAAGCTACTCAAGAAAAGTATGTTCTGGAAGAAGCTCAAAGACAAGCTGCCAGAGATCGAAAAACAAAAA
  CTCAAGAGTGGGTTTGTAAGTTATTTGAACTTGACCCACTCACTGGAGAATGGCACTATAAGTTTTCAGA
  TACTCGACCATGGGACCCACTTAATGATATGATACAATTTGAAAAAGATGGAGTTATCCAGACCAAAGTG
  AAACATCGCACTCCAATGGTTAGTGTTCCAAAAATGAAACATAAACCAACAAGGCAGCAGAAGAAAGTGG
  TAAAAGGCTATTCTTCCCCAGAACCTGACATCCAGGATTCCTCTGGAAGTGAAGCTCAATCTGTAAAACC
  AAGTACACGAAGGAAGAAGGGGATAGACCTGGGAGACATTCAGAGTTCCATTGAGTCTATAAAGCAGACA
  CAGGAAGAGATTAAAAGAAATATTATGGCTCTTCGAAATCATTTACTTTCAAGTACACCTGCTACAGATT
  ATTTTCTGCAACAGAAAGACTACTTCGTCATTTTCCTTCTGATTTTGCTTCAAGTCATAATAAACTTTAT
  ATTTAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175489
• Insert Size: 2670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175489.3, NP_780698.2
• RefSeq Size: 7152 bp
• RefSeq ORF: 2670 bp
• Locus ID: 237542
• UniProt ID: B9EJ86
• Cytogenetics: 10 D1
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (Osbp) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal Osbp-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created using sequences from two different strains, C57BL/6J and C57BL/6J x DBA/2J F1, because no single transcript from the same strain was available for the full length of the gene.