Lpin2 (NM_022882) Mouse Untagged Clone

CAT#: MC222426

Lpin2 (untagged) - Mouse lipin 2 (Lpin2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lpin2
• Synonyms: 2610511G02Rik; AI481352; AW742896
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222426 representing NM_022882
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTATGTGGGCCAGCTGGCTGGGCAGGTGCTCGTCACTGTGAAGGAACTCTACAAGGGCATTAACC
  AAGCCACGTTGTCTGGATGCATCGATGTGGTCGTGGTGAGGCAGCAGGATGGCTCCTACCAGTGCTCGCC
  TTTTCACGTACGCTTCGGGAAGCTGGGTGTCCTGAGGTCCAAGGAGAAAGTGATTGACATAGAAATCAAT
  GGCAGTGCTGTGGATCTTCACATGAAATTGGGTGATAATGGGGAAGCCTTCTTTGTAGAGGAGACTGAAG
  AAGAATATGAAAAATTACCTGCTTATCTTGCCACCTCACCAATTCCCACTGAAGACCAGTTCTTTAAACA
  TATTGAAACCCCTTTGGTGAAATCAAGTGGAAATGAAAGGCCAGCTCAGAGTTCAGACGTTTCTCACACC
  TTGGAATCAGAGGCAGTTTTCACTCAGAGTTCTGTGAAAAAGAAGAAACGAAGGAGAAAGAAGTGCAAAC
  AGGACAATAGGAAGGAGGAGCAGGCAGCTTCCCCTGTTGCAGAAGATGTAGGTGATGTGGGTGTGAGCTC
  AGATGATGAGAAGAGAGCCCAGGCAGCAAGAGGATCTTCAAATGCTTCCTTAAAGGAAGAGGACTACAAG
  GAGCCTTCACTCTTCCATTCTGGGGATAACTACCCCTTATCTGATGGAGATTGGTCCCCATTAGAAACCA
  CCTACCCTCAGGCTGTGTGCCCCAAGAGTGACTCTGAGCTGGAGGTGAAGCCATCTGAGAGCCTCCTCAG
  ATCTGAGCCGCACATGGAGTGGACGTGGGGCGGGTTCCCAGAGTCCACCAAGGTCACCAAAAGAGAACGG
  TATGACTATCATCCAAGGACAGCTACGATTACACCATCAGAGAACACACATTTCAGGGTAATTCCCAGTG
  AAGACAGCCTCATAAGAGAAGTTGAAAAGGATGCTACTGTTGAAGATACTACCTGTACCATAGTGAAACC
  CAAACCTAGAGCCCTGTGTAAGCAACTGAGTGATGCAGCGTCTACTGAGCTTCCCGAATCACCTCTTGAA
  GCACCTCAGATTTCATCGTTATTAGATGCAGACCCTGTTCCCAGCCCATCAGCAGAGGCTCCCTCAGAAC
  CCAAACCAGCTGCTAAAGACTCACCAACAAAAAAGAAAGGTGTTCACAAAAGAAGCCAGCACCAGGGACC
  TGATGACATTTACCTTGATGACTTAAAGGCTCTTGAGCCTGAAGTGGCGGCTCTCTATTTCCCTAAAAGT
  GACACGGATCCAGGTTCCAGGCAGTGGCCTGAGTCTGACACATTCTCTGGTTCTCAGTCCCCACAGTCTG
  TGGGGAGTGCAGCTGCGGACAGTGGCACTGAATGCCTCTCAGACTCTGCCATGGACTTGCCTGATGTAAC
  CCTCTCCCTCTGTGGAGGCCTCAGTGAGAATGGAGAGATTTCTAAAGAGAAGTTCATGGAGCATATCATC
  ACTTACCATGAGTTTGCAGAAAACCCTGGCCTTATCGACAACCCAAACCTCGTGATCCGGATATATAACC
  GTTACTACAACTGGGCGTTGGCTGCTCCCATGATCCTTAGCTTACAGGTATTTCAGAAGAGTTTGCCTAA
  GGCCACCGTTGAGTCCTGGGTTAAAGACAAGATGCCAAAGAAATCTGGTCGATGGTGGTTTTGGCGGAAA
  AAAGAAAGTATGATCAAACAGTTGCCAGAGACCAAGGAGGGAAAATCTGAGGTCCCTCCAGCAAATGACC
  TGCCTTCCAATGCTGAGGAGCCAACCAGTGCCAGACCTGCAGAGAATGACACTTCTAGTGACGAGGGGTC
  ACAGGAGTTGGAAGAAAGCATCAAAGTTGACCCCATCACCGTAGAGACACTGAGTCACTGTGGGACGGCC
  TCATATAAGAAGTCTCTCCGACTCTCCTCGGACCAGATAGCAAAACTGAAGCTCCATGATGGCCCCAATG
  ACGTGGTATTCAGTATCACAACCCAGTATCAGGGCACCTGTCGGTGTGCAGGGACCATCTACCTGTGGAA
  CTGGAATGACAAAGTCATCATCTCTGACATCGACGGAACAATAACCAAGTCTGATGCTTTGGGGCAGATT
  CTCCCACAGCTGGGTAAAGACTGGACGCATCAGGGCATAGCTAGGCTCTACCATTCCATCAATGAGAATG
  GCTACAAGTTTCTGTACTGTTCTGCACGTGCCATCGGCATGGCCGACATGACCCGTGGTTATCTGCACTG
  GGTCAATGATAAGGGGACGATCTTGCCTCGAGGCCCTCTGATGCTGTCTCCCAGCAGCTTGTTCTCTGCC
  TTCCACAGGGAAGTGATAGAAAAGAAACCAGAGAAGTTCAAAATTGAGTGTCTGAATGATATTAAGAACT
  TGTTTGCCCCGTCCAGGCAGCCCTTCTATGCTGCCTTTGGAAACCGTCCCAACGATGTCTATGCTTACAC
  ACAAGTCGGAGTTCCAGACTGTAGGATATTTACTGTGAATCCAAAGGGTGAATTAATCCAAGAGAGGACC
  AAAGGGAACAAATCATCGTATCACAGGCTGAGTGAGCTTGTGGAACACGTGTTCCCACTTCTCAGTAAGG
  AGCAGAATTCTGCCTTTCCATGCCCAGAGTTCAGCTCCTTCTGCTACTGGCGAGACCCAATCCCTGACCT
  GGACCTGGATGACCTGGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022882
• Insert Size: 2682 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022882.4, NP_075020.2
• RefSeq Size: 5651 bp
• RefSeq ORF: 2682 bp
• Locus ID: 64898
• UniProt ID: Q99PI5
• Cytogenetics: 17 E1.3
• Gene Summary: Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.