Spata5 (NM_021343) Mouse Untagged Clone

CAT#: MC222423

Spata5 (untagged) - Mouse spermatogenesis associated 5 (Spata5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spata5
• Synonyms: 2510048F20Rik; C78064; Spaf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222423 representing NM_021343
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAAGAAGAACCGAAAGCGGCAGAAGGAGGGCGCGGAGGGCGCCTCGCCCTCGCTCTCTGCGG
  CTCCGTCCCGCTCAGGGACCTCGACTCCTTTCGCCCAGCCGAGCCCGGCGGCGCCGGGGATGCTGGTGGT
  GACCAACTTCCTTGAGAAAGATGGCAAAGTTCCTAAGACATTCCAGAATTCCCTTGTTCATCTTGGACTT
  AACACTATGAAGTCTGCAAACATATGTATAGGTCGGCCAGTGCTCCTTACCAGTTTGGATGGAAAGCAGG
  AGGTTTATACAGCCTGGCCAGTTGCAGGATTTCCTGGAGGCAAGGTTGGCCTGAGTGAAATGGCACAGAA
  GAATGTGGGTGTGAGAGCTGGTGAGACCATCCAGGTCCAGCCTCTTTTGGGTGCTGTGCTTCAGGCTCAG
  GAAATGGACTTGGCGCTGAGTGACAAAGATAAGGAAATTAATGAAGAAGAACTGACTGGTTGTATTCTGA
  GAAAACTAGATGGCAAGATTGTTTTACCAGGCAACTTTCTATACTGTACATTCTATGGACGACTCTGCAA
  GTTACAAGTGTTGCAAGTGAAAGGGACAGATGGCACAACATTGGGAAAGCTTCAGAGTGCCTCTGGCACT
  GATGCCCAGGGAATGGCCTCTGAACATTCTAGCATGGAAAGCAGCGATGTGGATCTATCCTTCCAGCTAA
  GCCAGTTAGATCTGAAAGAACCTCAGAGCCCATCATCACAGAGTACTCCCTGTAAACCCACTAATGACAG
  AACTGTGAATAAAGCTGGTGAAGTTTTGTTGGATGTCACACAGAGCCCTAGGGATGGAAGTGGACTTGGA
  CTAGAGGAAAGCACAGGTCTTAAGTGTAGTTTTGACTCATCCAAAGAAGGAAATACACAACCTGTCAGTG
  AAGAGAAACTACTAAAACCTGCTAGTGCAGGGACAAAGAGCAATACTGATACTTTTTATTTTATTTCTTC
  AACAACAAGAATCAATCTTCGAAAAATTTGTACAAATTCAAAAGAACAAGACAGTCAGTTCAAAGTAACT
  TATGACATGATTGGAGGCTTAAACAGTCAGCTGAAAGCAATAAGAGAAATAATTGAATTGCCTCTCAAGC
  AGCCTGAACTTTTTAAGAGTTACGGAATTCCAGCGCCTAGAGGGTTATTACTCTATGGTCCTCCAGGTAC
  CGGAAAGACAATGATTGCTAGGGCTGTTGCTAATGAAGTTGGAGCTTATGTTTCTGTAATTAATGGTCCT
  GAAATTATAAGCAAATTCTACGGTGAGACTGAAGCAAGGTTACGCCAGATATTTGCTGAAGCTACTCTAC
  GACACCCATCAATTATTTTTATTGATGAACTGGATGCACTTTGCCCTAAAAGAGAAGGAGCTCAGAGTGA
  AGTGGAAAAGAGAGTTGTAGCTTCACTCTTAACATTGATGGATGGCATTGGCTCAGAAGGAAGTGAAGGA
  CGGGTATTAGTCCTTGGGGCCACAAATCGCCCTCAAGCACTAGATGCTGCACTCAGACGACCTGGGAGAT
  TTGATAAAGAGATTGAGATTGGCATTCCTAATGCCCAAGACCGGCTTGATATTCTCCAGAAACTACTCCG
  AAGGGTGCCACATTTGCTCACTAAAGCTGAGCTTCTGAGGTTGGCAAATAATGCCCATGGTTATGTTGGC
  GCAGACTTGAAAGCGTTGTGTAATGAGGCAGGTCTGCATGCCTTGCGAAGAGTCCTGAGGAAACAGCCTA
  ACCTCCCTGATAGCAAGGTGGCTGGCATGGTGAAGATTACTCTAAATGATTTCTTACAGGGGATGAATGA
  CATCAGGCCTAGTGCTATGAGAGAGGTAGCAATTGATGTCCCAAATGTTTCCTGGTCAGATATTGGAGGA
  CTGGAAAATATCAAACTGAAATTGAAACAGGCTGTGGAATGGCCCTTGAAACATCCTAAGTCTTTCAACA
  GAATGGGTATTCAGCCACCCAAAGGAGTTCTCTTGTATGGGCCACCTGGATGCTCAAAAACAATGATAGC
  AAAAGCTTTGGCCAATGAAAGTGGGTTGAACTTTCTAGCTATAAAGGGGCCCGAATTAATGAATAAATAT
  GTTGGTGAATCTGAAAGAGCAGTTAGAGAGATATTCCGTAAAGCAAGAGCAGTGGCACCGTCCATTATCT
  TTTTTGATGAACTTGATGCCCTGGCTGTTGAAAGGGGCAGTTCTTCAGGTGCCGGGAATGTTGCAGACCG
  TGTTTTGGCTCAGCTGTTAACGGAAATGGATGGGATTGAACAGCTAAAAAATGTGACAGTTTTGGCAGCT
  ACTAATCGCCCAGATAGAATAGACAAGGCTTTGATGAGGCCTGGAAGAATTGACAGGATCATTTATGTGC
  CCTTACCGGACGCAGCAACCAGAAGGGAAATACTGAACCTGCAGTTCCACTCAATGCCGATCAGTAACGA
  GGTTGACCTGGATGAACTTGTCCTCCAAACTGACACCTACTCAGGAGCAGAGATCATAGCAGTCTGCAAG
  GAGGCGGCTCTCCTAGCTCTGGAAGAAAACATTAAAGCTGACTGCATTATGAAGAGGCATTTCACTCAAG
  CCTTGAGCATTGTGACACCCAGGATCCCAGAGTCACTGAGACGCTTTTATGAAGATTACCAAGAAAAGAG
  TGGACTACACACAGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021343
• Insert Size: 2679 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021343.3, NP_067318.2
• RefSeq Size: 3255 bp
• RefSeq ORF: 2679 bp
• Locus ID: 57815
• UniProt ID: Q3UMC0
• Cytogenetics: 3 B
• Gene Summary: ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (By similarity). May be involved in morphological and functional mitochondrial transformations during spermatogenesis (PubMed:10734318).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.