Musk (NM_001037127) Mouse Untagged Clone

CAT#: MC222416

Musk (untagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Musk
• Synonyms: Mdk4; Mlk; Ns; Nsk1; Nsk2; Nsk3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222416 representing NM_001037127
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGAGCTTGTCAACATTCCACTGTTACAGATGCTCACCCTGGTTGCCTTCAGCGGGACTGAGAAAC
  TTCCAAAAGCCCCTGTCATCACCACGCCTCTTGAAACTGTAGATGCCTTGGTTGAAGAAGTAGCGACTTT
  CATGTGTGCCGTGGAATCCTACCCTCAGCCCGAGATTTCTTGGACCAGAAATAAAATTCTCATTAAGCTG
  TTTGACACCCGCTACAGCATCCGGGAGAATGGTCAGCTCCTCACCATTCTGAGCGTGGAAGACAGTGATG
  ATGGCATCTACTGCTGCATAGCCAACAATGGAGTGGGAGGAGCCGTGGAGAGTTGTGGTGCCCTGCAAGT
  GAAGATGAAACCTAAAATAACTCGTCCTCCCATTAATGTAAAAATAATAGAGGGATTGAAGGCAGTTCTG
  CCGTGCACTACGATGGGTAACCCCAAACCATCTGTGTCCTGGATCAAGGGGGACAATGCTCTCAGGGAAA
  ATTCCAGAATCGCAGTTCTTGAATCTGGGAGCTTAAGGATCCATAATGTGCAAAAGGAAGATGCAGGACA
  GTACCGCTGTGTGGCCAAAAACAGCCTGGGCACAGCTTACTCCAAACTGGTGAAGCTGGAAGTGGAGGAA
  GACAGAGAACCTGAGCAGGACGCTAAAGTTTTTGCAAGAATCCTGCGTGCTCCTGAATCCCACAATGTCA
  CCTTTGGTTCCTTTGTAACCCTACGCTGCACAGCAATAGGCATCCCTGTCCCCACCATCAGCTGGATTGA
  AAACGGAAATGCTGTTTCTTCAGGTTCCATTCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTC
  CAGCTCTTCATCACAAAGCCAGGACTCTACACATGCATAGCTACCAATAAGCACGGAGAAAAGTTCAGTA
  CCGCAAAGGCTGCAGCCACTGTCAGCATAGCAGTCCCTCCTCCGTGGTTTTCTATGGATACTTCTTTCCT
  ATGGACAGAATGGAGTAAGTCACAGAAAGACAGCCAAGGCTACTGTGCCCAGTACAGAGGGGAGGTGTGT
  GATGCAGTCCTGGCGAAAGATGCTCTTGTCTTCTTCAACACCTCCTACCGGGACCCCGAGGACGCCCAGG
  AGCTGCTGATCCACACTGCGTGGAATGAGCTGAAGGCTGTGAGTCCACTGTGCCGGCCAGCTGCTGAGGC
  TCTGCTGTGTAACCACCTCTTCCAAGAGTGCAGCCCTGGAGTGGTACCTACTCCCATGCCCATTTGCAGA
  GAGTACTGCCTGGCGGTAAAGGAGCTCTTCTGTGCAAAGGAATGGCAGGCAATGGAAGGAAAGGCCCACC
  GGGGCCTCTACAGATCTGGGATGCATCTCCTTCCGGTACCAGAGTGCAGCAAGCTTCCCAGCATGCACCG
  GGACCCCACAGCCTGCACAAGACTGCCATATTTAGATTATAAAAAAGAAAACATAACAACATTCCCGTCA
  ATAACGTCCTCCAGGCCGAGCGCGGACATTCCAAACCTGCCTGCCTCCACCTCTTCCTTTGCCGTCTCGC
  CTGCGTACTCCATGACCGTCATCATCTCCATCGTGTCCAGCTTTGCCCTGTTTGCTCTTCTCACCATCGC
  TACTCTCTATTGCTGCCGAAGGAGGAAAGAATGGAAAAATAAGAAAAGAGAGTCGACCGCGGTGACCCTC
  ACCACGTTGCCTTCCGAGCTCCTGCTGGATAGGCTCCATCCCAACCCCATGTACCAGAGGATGCCACTCC
  TTCTGAATCCTAAGTTGCTCAGCCTGGAGTATCCGAGGAATAACATTGAGTATGTCCGAGACATCGGAGA
  GGGGGCGTTTGGAAGAGTCTTCCAAGCAAGGGCCCCTGGCTTGCTGCCTTATGAACCTTTCACTATGGTG
  GCCGTGAAGATGCTTAAGGAAGAGGCCTCTGCAGACATGCAAGCGGACTTTCAGAGGGAGGCGGCCCTCA
  TGGCAGAGTTTGACAACCCCAACATTGTGAAACTCTTAGGTGTGTGTGCCGTTGGGAAGCCGATGTGTCT
  GCTCTTTGAATATATGGCCTATGGTGACCTCAATGAGTTCCTCCGAAGTATGTCCCCGCACACTGTTTGC
  AGCCTCAGCCACAGTGACCTGTCCACGAGGGCTCGGGTGTCTAGCCCTGGTCCTCCACCACTGTCCTGTG
  CAGAACAGCTCTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCCTACCTTTCAGAGCGCAAGTTTGTCCA
  CCGGGACTTAGCTACCAGGAACTGCCTGGTTGGGGAGACCATGGTGGTGAAAATTGCAGACTTTGGCCTC
  TCCAGGAACATCTATTCCGCAGACTACTACAAAGCTGATGGAAATGACGCCATCCCTATCCGCTGGATGC
  CGCCCGAGTCTATCTTCTACAACCGCTACACCACTGAGTCGGATGTATGGGCCTATGGTGTGGTCCTCTG
  GGAGATCTTCTCCTATGGGCTGCAGCCCTACTATGGAATGGCCCACGAGGAGGTCATTTACTATGTGAGA
  GATGGCAACATCCTCGCCTGCCCTGAGAACTGCCCCTTGGAACTGTACAACCTCATGCGCCTGTGTTGGA
  GCAAGCTGCCTGCTGATAGACCCAGCTTCTGCAGTATCCACAGGATCCTGCAGCGCATGTGCGAGAGAGC
  AGAGGGAACGGTGGGTGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001037127
• Insert Size: 2682 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037127.2, NP_001032204.2
• RefSeq Size: 3422 bp
• RefSeq ORF: 2682 bp
• Locus ID: 18198
• Cytogenetics: 4 31.87 cM
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.