Nedd4 (NM_010890) Mouse Untagged Clone

CAT#: MC222415

Nedd4 (untagged) - Mouse neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nedd4
• Synonyms: AA959633; AL023035; AU019897; E430025J12Rik; mKIAA0093; Nedd4-1; Nedd4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222415 representing NM_010890
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCGGACATGGCAGCCGACGAGTCGGAGGCCCCAGTACTCTCGGAGGACGAGGTATGGGAGTTTT
  GCCTGGATAAGACAGAAGATGGTGGCGGATCCCCCGGAAGTGATGTTACAGATACTTGTGAGCCTCCATG
  TGGATGCTGGGAGTTGAATCCGAATTCCCTGGAAGAGGAGCACGTGCTGTTCACTGCTGATCCGTACCTG
  GAGCTCCACAACGATGACACACGAGTTGTGAGAGTGAAGGTTATAGCTGGCATAGGCCTGGCCAAGAAAG
  ACATCTTGGGAGCCAGTGATCCTTACGTAAGAGTGACATTGTATGACCCGATGAGTGGAATCCTTACCAG
  CGTGCAGACAAAAACTATCAAAAAGTCTTTGAATCCAAAATGGAATGAAGAAATACTGTTCAGGGTCCTT
  CCACAGCGACACCGCATTCTTTTCGAAGTGTTTGATGAAAATCGTTTGACAAGAGATGATTTCCTAGGTC
  AAGTGGATGTCCCTCTCTATCCTTTACCGACTGAAAACCCAAGAATGGAGAGACCATATACATTTAAGGA
  TTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGATTAAAAATGACTTATTTA
  CCTAAAAATGGCTCAGAAGATGAAAATGCAGACCAGGCTGAGGAGTTAGAGCCTGGCTGGGTTGTTTTGG
  ACCAACCAGATGCTGCCACTCATTTGCCGCATCCACCAGAACCCTCTCCCCTACCTCCAGGATGGGAAGA
  GAGGCAGGATGTCCTTGGAAGGACCTACTACGTAAACCATGAATCTAGAAGAACACAGTGGAAAAGGCCA
  AGCCCTGACGATGACCTCACGGATGAAGACAATGATGATATGCAGCTGCAAGCGCAGCGAGCATTCACCA
  CCAGGCGGCAGATATCGGAGGATGTGGATGGCCCTGACAACCGGGAGTCCCCTGAGAATTGGGAAATCGT
  ACGAGAAGATGAAAACACCGAGTATAGTGGTCAGGCTGTCCAGTCACCTCCATCGGGTCACATTGATGTG
  CAGACTCACCTTGCAGAAGAGTTTAATACCAGACTTGCTGTGTGTGGAAATCCAGCCACCAGCCAGCCGG
  TTACCAGCTCAAATCATTCCAGCAGAGGAGGCAGCTTGCAGACCTGTATCTTTGAGGAACAGCCTACACT
  TCCTGTGCTTTTGCCTACTTCATCTGGATTGCCACCAGGTTGGGAAGAAAAACAAGATGACAGAGGAAGA
  TCATACTATGTAGACCACAACTCTAAAACCACCACATGGTCCAAGCCCACCATGCAGGATGATCCAAGAT
  CGAAAATCCCTGCTCATCTGAGAGGAAAGACTGACTCCAATGACCTGGGACCCTTACCTCCAGGCTGGGA
  AGAAAGAACCCACACAGATGGGCGAGTCTTCTTCATAAACCACAATATAAAGAAGACCCAGTGGGAAGAT
  CCTCGCCTGCAGAACGTGGCAATCACTGGACCAGCAGTGCCCTACTCCAGAGATTACAAGAGAAAGTACG
  AGTTCTTCAGAAGGAAGCTCAAGAAGCAGACTGACATTCCAAACAAATTTGAAATGAAGCTTCGCCGCGC
  AAACATTCTGGAGGATTCTTACCGGAGGATTATGGGTGTGAAGAGAGCTGACTTGCTCAAGGCCAGACTC
  TGGATTGAGTTTGATGGTGAAAAGGGCCTTGACTATGGAGGGGTTGCCAGAGAGTGGTTCTTCCTCATCT
  CGAAGGAAATGTTCAACCCTTACTACGGCCTGTTTGAATATTCTGCTACGGATAATTACACCCTACAGAT
  AAATCCTAACTCGGGCTTGTGTAATGAAGATCACCTCTCATACTTCAAGTTCATTGGCCGTGTGGCTGGG
  ATGGCAGTTTATCATGGCAAGCTGTTGGATGGGTTTTTCATCCGTCCGTTTTACAAGATGATGCTTCAGA
  AACTGATAACACTGCACGACATGGAGTCCGTGGATAGTGAATATTACAGTTCTCTGCGATGGATTCTTGA
  AAATGACCCGACGGAGCTGGACCTGAGATTTATCATAGATGAAGAACTTTTTGGACAGACACATCAGCAC
  GAACTGAAAACCGGAGGATCAGAGATTGTTGTCACCAATAAGAACAAAAAGGAGTATATCTACCTTGTAA
  TACAATGGCGATTTGTGAACCGTATCCAGAAGCAAATGGCAGCTTTTAAAGAGGGATTTTTTGAACTGAT
  ACCACAGGATCTCATCAAGATATTTGATGAAAATGAGCTAGAGCTTCTCATGTGTGGTCTGGGAGATGTG
  GATGTGAACGACTGGCGGGAACACACAAAATACAAAAATGGCTACAGCATGAACCACCAGGTCATCCACT
  GGTTCTGGAAGGCTGTTTGGATGATGGATTCGGAAAAAAGAATACGCTTACTTCAGTTTGTCACTGGCAC
  ATCCCGTGTGCCGATGAATGGGTTTGCTGAACTCTATGGCTCGAATGGACCACAATCCTTCACAGTGGAA
  CAATGGGGCACCCCTGATAAGCTGCCAAGAGCACACACCTGCTTCAATCGCCTGGACCTGCCACCCTACG
  AATCCTTTGACGAACTCTGGGATAAACTTCAGATGGCAATTGAGAACACACAGGGCTTTGATGGCGTTGA
  TTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010890
• Insert Size: 2664 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010890.3, NP_035020.2
• RefSeq Size: 5494 bp
• RefSeq ORF: 2664 bp
• Locus ID: 17999
• UniProt ID: P46935
• Cytogenetics: 9 40.08 cM
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (By similarity). Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Involved in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19193720). Predominantly involved in ubiquitination of membrane bound forms of ERBB4 rather than processed precursors and intermediate membrane-anchored 80 kDa fragments (m80HER4), with a lesser role in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19047365). Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (By similarity). Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity).[UniProtKB/Swiss-Prot Function]