Hic1 (NM_010430) Mouse Untagged Clone

CAT#: MC222411

Hic1 (untagged) - Mouse hypermethylated in cancer 1 (Hic1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hic1
• Synonyms: AA408311; HIC-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222411 representing NM_010430
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTACAAGAATGCGCCACCATCCCTGGCTTAAGAGTTCCTCATTTGAAGACAGAACCTTCCCCTCCC
  CCCCCCCGAATTGGCGCGATATAACATATAAATATGTACTCGGGAGCACTCAGCACAAAACCCCCAGGTT
  TATGGGACTCCCAGGTCCGATATCTGAGTCTCCAGGTGGGCACATCTGGAAGCGCGCGAACCTGCAAGAT
  CTGCTCAGCTCCGCATCTGCAAGCCTCTTGGCCCAGGTGTGCGCCAGAGGTCGCAGCCCAGCCGCACACA
  GTCCCCGCGTGGCCGCGAGGTGGCGCCATGGCCGCGGCAGCGTCTGCCGGTTCGGGCCGCTCCAGATAAG
  AGTGTGCGGAAAGCGCGGAGGGGCTGAGACGCGACCAGGACGCGGGGAGGACGGACCGGCAAGACAGACC
  GACCGGGGGCCCGGCGGGCGGAGGGCAGCGCACTGCAGCCACGTCCCCCCCTGGATCCGCCGGCAGCCGG
  GCCCGGGGCTTCCGACATGCCCCCCAGGAGAGTGTGCTGGGCAGACGATGCTGGACACGATGGAGGCGCC
  TGGCCATTCGAGGCAGCTACTGCTGCAGCTCAACAATCAGCGCACCAAGGGCTTCTTGTGCGACGTGATC
  ATCGTGGTGCAGAATGCCCTCTTCCGCGCGCACAAGAACGTGCTGGCGGCCAGCAGCGCCTACCTCAAGT
  CCCTGGTGGTGCATGACAACCTGCTAAACCTGGACCATGACATGGTGAGCCCGGCCGTGTTCCGCCTGGT
  GCTGGACTTCATCTACACCGGCCGCCTGACTGACAGTGTTGAGGCCGCAGCAGCAGCAGCGGTGGCCCCG
  GGCGCGGAGCCGAGCCTGGGCGCTGTGCTGGCTGCTGCCAGCTACCTGCAGATCCCTGACCTCGTGGCTC
  TGTGCAAGAAGCGCCTCAAACGCCACGGCAAGTACTGCCACCTGCGGGGAGGAGGCAGCGGCGGCGGCGG
  CTACGCTCCTTACGGGCGGCCCGGCCGGGGCTTGAGGGCTGCCACGCCCGTCATCCAGGCTTGCTACTCG
  TCCCCGGCCGGGCCACCGCCGCCGCCTGCCGCCGAGCCGCCGTCGGGCCCCGATGCAGCCGTCAACACCC
  ACTGCGCTGAGCTATATGCTTCAGGCCCGGGCCCAGCAGCCTCACTCTGCGCCCCGGAGCGTCGCTGCTC
  CCCGCTTTGCGGCCTGGATCTGTCCAAGAAGAGCCCGCCAGGTTCCTCGGTCCCCGAGCGACCGCTTAGT
  GAGCGCGAACTGCCTCCACGCCCGGATAGCCCTCCCGGTGCGGGGCCCGCAGTCTACAAGGAGCCATCAC
  TCGCCCTGCCCCCGCTGCCGCCTTTGCCCTTCCAAAAGCTGGAGGAGGCCGTACCGACTCCAGACCCGTT
  TCGAGGAAGCGGTGGCAGTCCGGGACCCGAGCCCCCCGGCCGCCCCGACGGCTCCAGCCTCCTCTACCGC
  TGGATGAAGCACGAGCCAGGCCTGGGTAGTTATGGCGATGAACTGGTCCGGGATCGAGGCTCCCCGGGTG
  AGCGCCTGGAGGAGCGCGGTGGGGATCCTGCCGCCTCACCCGGGGGCCCCCCGCTGGGCCTGGTACCCCC
  GCCACGCTACCCCGGGAGCCTGGACGGGCCAGGCACAGGAGCAGACGGCGACGACTATAAGAGCAGCAGC
  GAGGAGACCGGTAGCAGCGAAGACCCCAGCCCACCCGGCGGCCACCTGGAGGGCTACCCATGCCCGCACT
  TGGCTTATGGTGAGCCTGAGAGCTTTGGTGACAACCTGTACGTGTGCATCCCATGTGGCAAAGGCTTCCC
  CAGCTCGGAACAGCTGAATGCACACGTGGAGGCTCACGTAGAGGAAGAAGAGGCGTTATATGGCAGGGCA
  GAGGCTGCTGAGGTGGCTGCTGGGGCCGCCGGCCTTGGGCCCCCCTTTGGTGGCGGTGGGGACAAGGTCA
  CTGGGGCGCCGGGCGGACTAGGAGAGCTGCTGCGGCCATACCGCTGCGCGTCCTGCGACAAGAGCTACAA
  GGACCCGGCCACGCTGAGGCAGCACGAGAAGACACACTGGCTGACAAGGCCCTATCCGTGTACCATCTGC
  GGAAAGAAGTTCACGCAACGCGGAACCATGACACGCCATATGCGCAGCCACTTGGGCCTGAAGCCCTTTG
  CGTGCGACGCGTGCGGCATGCGCTTCACCCGCCAGTATCGCCTCACGGAGCACATGCGCATCCACTCTGG
  AGAGAAGCCCTACGAGTGCCAGGTGTGCGGTGGCAAGTTTGCTCAACAGCGCAACCTCATCAGCCATATG
  AAGATGCACGCTGTAGGTGGCGCGGCCGGCGCGGCCGGGGCGCTGGCTGGCCTGGGGGGACTACCTGGCG
  TCCCTGGCCCTGACGGCAAGGGCAAGCTCGACTTCCCTGAGGGTGTCTTTGCTGTGGCCCGCCTCACAGC
  TGAACAGCTGAGTCTGAAGCAGCAGGACAAGGCAGCTGCTGCCGAGCTGCTGGCGCAGACCACGCACTTC
  CTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACCCGCTGGCTAAATTCACTGCTGAGCTAGGACTCA
  GCCCAGACAAGGCGGCAGAGGTGCTGAGCCAGGGTGCGCACTTGGCCGCAGGACCGGACAGCCGAACCAT
  CGACCGTTTCTCTCCCACCTAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_010430
• Insert Size: 2682 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010430.2, NP_034560.2
• RefSeq Size: 4247 bp
• RefSeq ORF: 2682 bp
• Locus ID: 15248
• Cytogenetics: 11 45.76 cM
• Gene Summary: Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from ACKR3, FGFBP1 and EFNA1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.