Bbs9 (NM_181316) Mouse Untagged Clone

CAT#: MC222404

Bbs9 (untagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbs9
• Synonyms: E130103I17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222404 representing NM_181316
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGAATTTGACCAGGGCT
  GCTTATGCTTGGCTGATGTGGACAACAGTGGGAACGGCCACGATAAAATCATTGTGGGCAGCTTCATGGG
  CTACCTGAGAATCTTTAGTCCCCATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTGAAGATTTACTTCTA
  GAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTCGGGCACCGAGATGCTTC
  ATCTGGCCGTGCTGCACTCCCGGAAGCTCTGTGTCTACTCCGTCTCAGGGACATTGGGAAATGTGGAACA
  TGGAAACCAATATCAGATAAAACTAATGTATGAGCACCATCTTCAGAGGACGGCCTGCAATATGACGTAT
  GGACCCTTTGGTGGTGTAAAAGGTCGGGATTTAATTTGTATCCAGTCTCTGGATGGGATGCTGATGGTGT
  TTGAGCAGGAAAGCTATGCGTTTGGGAGGTTTCTCCCTGGTTTTCTTCTGCCTGGCCCTCTTGCTTACAG
  TCCCCGCACGGATTCCTTCATAACTGTGTCTTCTTGCAGACAAGTGGAAAGTTACAAGTACCAGGTACTT
  GCCTTTGCCACAGATGCAGACAAGAAGCAGGAGATGGAACAGCAAAAACTTGGTTCTGGAAAGCGGCTAG
  TTGTGGACTGGACTCTGAATATTGGCGAGCAAGCCCTTGATATCTGTATCTTCCCTTTGAACCAATCTGC
  ATCTTCTGTTTTTGTTCTTGGTGAGAGAAACTTCTTTTGCCTAAAGGATAATGGGCAGATTCGATTCATG
  AAGAAGCTCGGTTATAACCCCAGTTGTTTTCTACCGTACTGCTCAGTTTCTGAAGGTACAATAAATACTT
  TGATTGGAAACCATAACCACATGCTGCATATTTACCAGGACGTGACACTGAAGTGGGCCACACAACTCCC
  CCACGTCCCCGTGGCCGTCAGAGTAGGCTGTTTTCATGATTTGAAGGGAGTGATAGTGACTCTCAGTGAT
  GACGGTCACCTGCAGTGCTCGTATCTGGGGACAGACCCTTCTTTGTTCCAGGCTCCAAAAGTTGAATCAA
  GAGAGCTCAACTATGATGAACTTGATGTGGAATTGAAAGAACTTCAGAAAATCATCAAAGATGTAAAGTT
  GCAAGGTGTTTGGCCCTTGACTGAACAGGAAGATGACTTGAAAGTCTCTGCTTCAGTGTCTTCTACCCTG
  GATTCTGTGTCTCAAGCCACCAATGTGGAACCTGGAGCTGACTCTGTCCCGTCCATCACAGTGAAGATTA
  CCCTGCAGAACCGAGTGGTGTTGCAGAAGGTCAAGCTGTCTATCTATGTGCAGCCTCCACTACAGTTGAC
  TTGTGATCAGTTCACCTTTGACTTCACGGTTCCAGATATGACGAGTTCAGTAGCGTTTTCTGTGTACCTG
  AAGAGAAACTACACACCATCCGAGTTAGAAGGAAATGCTGTTGTTTCTTACTCCAGACCGACAGGTATTC
  CTCGGGTTGTCCAATGTAAATTTAGACTTCCTCTGAAACTGATTTGCCTACCTGGTCAGCCTTCAAAAAC
  TGCAAGCCACAAACTAACTATAGACACCAACAAATCTCCTGTCAGTCTGCTCGGTCTCTTCCCAGACTTT
  GCCAACCCATCAGATGACGACCAAGTGAATGTAATGGGCTTCCGTCTCCTAGGAGGCGCTCGAGTGACTC
  TTCTTGCTTCCAGAACATCCCAACGATACCGCATTCAGAGCGAACAGTTTGAAGATCTCTGGCTCATAAC
  CAATGAACTCATCCTCCGCCTTCAGGAACATTTTGAAAAACAGGGAACCAAAGACTTTTCGTGTTCTTTT
  TCTGGGTGTGTGCCCCTTCAAGAATATTTTGAGTTGATTGATCATCATTTTGAGCTGCGGATAAATGGTA
  AAAAATTGGAAGAACTTTTATCTGAAAGAGCTGTACAATTTCGGGCCATTCAGCGTCGGCTTCTGACAAG
  ATTCAGAGACAAGACCCCTGCCCCACTGCAGCACCTGGACACCCTGCTGGACGGGACATACAAGCAGGTG
  ATTGCTCTAGCAGATGCGATCGAGGAGAACCAGGACAGGCTGTTGCAGTCATTCTCAGGCCTGAAGAGTG
  CCACCCATTTGCTGATCCTGCTAATCAGGCTGTGGCAGAGGCTGAGCGCTGACCAGACTGCTATTCTGGA
  AGCAGCATTTCTGCCACTACAGGAAGACACGCAGGAACTGGGCTGGGAAGAAACCGTGGATGCTGCCATT
  GCCTACCTTTTGAAAACCTGCCTGTCGAAGAGTTCCAAGGAGCAGGCTTTGAACCTCAGCAGCCAACTGA
  ACATACCCAAAGATACCAGCCGGCTGAAGAAGCACATCACCCTGCTGTGTGATAGGTTAGCCAAAGGTGG
  GCGTCTCTGCGTGAGCACAGATGCAGCGGCCCCGCAGGCCATGGTTGTGCCAGGTGGCTGTACTCCAATC
  CCAGAGTCAGACCTAGAGGAAAGGTCACTAGATGACTCCACAGAGCTGTTTACCAACCACAAGCACCTCA
  TGACCGAGCCCCCCATGCCTGAAGTCTCAGCCCGCCAAGGAGTTTTGGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181316
• Insert Size: 2643 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181316.4, NP_851833.2
• RefSeq Size: 3382 bp
• RefSeq ORF: 2643 bp
• Locus ID: 319845
• UniProt ID: Q811G0
• Cytogenetics: 9 A3
• Gene Summary: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function]