Grm3 (NM_181850) Mouse Untagged Clone

CAT#: MC222392

Grm3 (untagged) - Mouse glutamate receptor, metabotropic 3 (Grm3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grm3
• Synonyms: 0710001G23Rik; Gprc1c; mGlu3; mGluR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222392 representing NM_181850
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGTTGACAAGACTACAAGTTCTTATGTTAGCTTTGTTTTCAAAGGGATTTTTAGTCTCTTTAG
  GAGATCACAACTTTATGAGGAGAGAAATTAAAATAGAAGGAGACCTTGTTTTAGGGGGCTTATTTCCTAT
  TAATGAAAAAGGCACTGGAACTGAAGAGTGTGGACGAATCAATGAAGACAGAGGTATCCAACGCCTGGAG
  GCCATGTTGTTTGCCATTGATGAAATCAACAAAGACAATTACTTGCTTCCAGGAGTGAAGCTGGGGGTTC
  ACATTTTGGATACATGTTCAAGAGACACCTATGCATTAGAGCAGTCACTGGAGTTTGTCAGAGCATCGTT
  GACTAAAGTGGATGAAGCTGAATATATGTGTCCTGATGGATCATATGCTATTCAAGAAAACATCCCACTA
  CTCATTGCAGGAGTCATTGGCGGTTCGTACAGCAGTGTTTCCATACAGGTGGCAAACCTGCTGAGGCTCT
  TCCAGATCCCTCAGATAAGCTACGCCTCCACCAGTGCCAAACTCAGCGACAAATCGCGCTATGATTATTT
  TGCCAGGACCGTGCCCCCTGACTTCTACCAGGCCAAAGCCATGGCCGAGATCTTGCGCTACTTTAACTGG
  ACCTATGTGTCCACTGTTGCCTCTGAAGGTGACTATGGGGAGACAGGGATTGAGGCCTTCGAGCAGGAAG
  CAAGGCTACGCAACATCTGCATCGCCACTGCTGAAAAGGTGGGGCGCTCCAACATCCGCAAGTCCTACGA
  CAGCGTGATCCGTGAGCTCCTGCAGAAACCTAACGCGCGAGTTGTGGTCCTGTTCATGCGCAGTGATGAC
  TCACGAGAGTTGATCGCTGCAGCCAGCCGCGTGAATGCTTCCTTCACCTGGGTGGCCAGCGATGGCTGGG
  GTGCACAGGAGAGCATTGTCAAGGGCAGTGAGCACGTCGCCTATGGAGCCATCACCCTGGAGCTGGCGTC
  CCACCCTGTTCGTCAGTTTGATCGCTACTTCCAGAGCCTCAACCCCTACAACAATCATCGTAACCCCTGG
  TTCCGAGACTTCTGGGAGCAGAAGTTCCAGTGCAGCCTCCAGAACAAGAGAAACCACAGACAGATTTGTG
  ACAAGCACCTGGCCATTGACAGCAGCAACTATGAACAAGAATCCAAGATCATGTTTGTGGTGAATGCAGT
  GTATGCCATGGCGCATGCGCTGCACAAAATGCAACGCACCCTCTGTCCCAACACCACCAAGCTCTGTGAT
  GCAATGAAGATCCTGGATGGAAAGAAGTTGTACAAAGATTATTTGCTGAAAATCAACTTCACAGCTCCAT
  TCAACCCAAATAAAGGAGCAGACAGCATTGTGAAGTTTGACACTTACGGAGACGGGATGGGAAGATACAA
  CGTGTTCAACTTCCAGCATATAGGTGGAAAGTATTCCTACTTAAAAGTTGGCCACTGGGCAGAAACTTTA
  TATCTAGATGTGGACTCTATTCATTGGTCCCGGAACTCAGTCCCCACTTCCCAGTGCAGTGATCCCTGTG
  CCCCCAATGAAATGAAAAACATGCAGCCAGGAGATGTTTGCTGCTGGATCTGCATCCCATGTGAGCCCTA
  TGAATACCTGGTTGATGAGTTCACCTGCATGGATTGTGGCCCTGGCCAGTGGCCCACTGCAGACCTATCT
  GGATGCTACAACCTTCCAGAGGATTACATCAGGTGGGAAGATGCCTGGGCAATAGGCCCAGTCACTATTG
  CCTGCCTGGGTTTTATGTGTACATGCATAGTCATAACTGTTTTTATCAAGCACAACAACACACCCTTGGT
  CAAAGCATCAGGCCGAGAACTCTGCTACATCTTGTTATTTGGAGTTAGCCTGTCCTATTGCATGACATTC
  TTCTTCATTGCTAAGCCATCGCCTGTCATCTGTGCATTGCGCCGACTTGGGCTTGGGACCTCCTTTGCCA
  TCTGTTATTCAGCTCTCCTGACCAAGACAAACTGCATCGCTCGCATCTTTGATGGGGTCAAGAATGGCGC
  TCAGAGGCCAAAATTCATCAGCCCCAGTTCTCAGGTTTTTATCTGCCTGGGTTTGATACTGGTGCAAATT
  GTGATGGTGTCTGTGTGGCTTATCTTGGAGACTCCAGGTACTAGAAGATACACCCTGCCAGAGAAGCGGG
  AAACAGTCATCCTAAAATGCAATGTCAAAGATTCCAGCATGTTGATCTCTCTGACCTATGACGTGGTTCT
  GGTGATTCTATGCACTGTGTATGCCTTCAAAACAAGGAAGTGTCCTGAAAACTTCAATGAAGCCAAGTTC
  ATAGGCTTCACCATGTACACCACCTGCATCATCTGGTTGGCATTCCTCCCTATATTTTATGTGACATCAA
  GTGACTACAGAGTGCAGACGACAACAATGTGCATCTCCGTTAGCTTGAGTGGTTTCGTGGTCTTGGGCTG
  TTTGTTTGCCCCCAAGGTGCACATTGTCCTGTTCCAACCCCAGAAGAATGTGGTCACACACAGACTTCAC
  CTCAACAGGTTCAGTGTCAGTGGAACTGCGACCACATATTCTCAGTCCTCTGCAAGCACATATGTCCCGA
  CAGTGTGCAACGGAAGGGAAGTCCTGGACTCCACCACCTCATCTCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181850
• Insert Size: 2640 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181850.2, NP_862898.1
• RefSeq Size: 3536 bp
• RefSeq ORF: 2640 bp
• Locus ID: 108069
• UniProt ID: Q9QYS2
• Cytogenetics: 5 A1
• Gene Summary: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.[UniProtKB/Swiss-Prot Function]