Faap100 (NM_027980) Mouse Untagged Clone

CAT#: MC222383

2310003H01Rik (untagged) - Mouse RIKEN cDNA 2310003H01 gene (2310003H01Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Faap100
• Synonyms: 2310003H01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222383 representing NM_027980
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCTTCCTCGCGCGTGCACTACCTGTCGGACTTCTGCTGCCCGCTCGGAGGCCGAGCGGCGG
  GGAAGCCGTTTGTGCTGCGGCATGAGGCTGAGGTCTTTCTGTCCACCGGACGCGAGTTCGTCTATGTGTA
  TGACCAGGAGGGCGGGCTGCTGACGGCGGTGTACCAGTTTCCCGACCAGGTATGGCACCTGCAGCTCCTG
  GCCATCCGCAGGGCACTCTACGTCCTGTGTGCCCGGACTGGCATCTACTGCCTGTCGTTGGACTCGCTGG
  ACAGGTCTGGGAGCCAGGCCTGTGAGGACAAAGAGGAAGCGGCTCCCCCCTACCCTGTGATCCATGTGGA
  CCCGGATGCGTGTGTCCTCCCTGACGCTGCCCTCTGTGCCTTCACCGTGCTGGATGATATGCTGGTCACC
  CTGGCACAGGGCCCCACCCAGTGGAAGATGCAGCTGTTTGAGCGTCCCTGTGCAGGGGAGGAGCCTCTGC
  CCAGAGGCCAGATTGGTGAGGTGGAGTTGTCCACTTGCACCCCACCAGGTGGGGTGCCAGAGAAGCCTGC
  AGCCCCCCGCTTCCTGCCCGTGCTGTGCTGCGTGTTCCCACCAGACTCCAGGGCACCTCATGGTCACCCC
  CAGGGCTGTGGGTGCTTCACGCTGGAGGAGGCTCTCTTTGGTCTACTCTTTGGAGTCGATGCCACCCTCC
  TGCAGTCCCCTGTGATCCTCTGCGGTCTCCCTGATGGCCAGCTTTGTTGTGTGGTCCTGAAGGCCTTGGT
  TACTTCTGGGTTAGCCCCTGGTGACCCAAAGGTCCTGGTCAAGATCCTCCACCACCTAGAGGAGCCTGTG
  ATCTTCATTGGGGCTTTGAGGGCAGAGCCACACGAGGAGGAAGCAGCAGGAGAGCTGCTGCCTGGCCAGC
  ATGAACACTCTGACTGCCTAGTAGCCCTTGGTCACCAGGGCCGGACACTGGCCATCAAGGCCAGCTGGAG
  TGAGTCAGGGAATCTGGTGCCGGAGCTGCGTGAGTATTGCCTCCCAGGGCCTGTGCTCTGTGCTGCCTGT
  GACAGGGATGGCCACGTGTACCACAGCACTCCCTCAGACCTCTGTGTGGTAGACTTGACTCGGAGAGACA
  GTCCTTGGAACCCTGAGAAGCCAGACGGGGCCATAGGAGGTCTACCCTCTGTGCTGTGCCCAGCCAGCTT
  GAACATCTGCAGCGCCCTGGCTCTGTGTGTGACAGCAAGGGCACCTACAGGTAGCACCGAGCTCCTCGCC
  CTGTCTTCTAAAGGACGCCTGATCACCTGCAGTCTGGACTTGAACTCTGAGGCGCCTGTGCCTGCCAAAA
  TGGCCATGGCAAATGCAGGCCAGAAAATTAAGGAGCTGCTCTTGGACATCGGTGATGTCTCTGAAAGGGT
  GTCCTTTCTGAAGAAAGCTGTTGACCAACGCAACAAGGCCATCACAAGTCTCAACGAGGCCATGAACGTG
  AGCTGTGCCCTGCTGTCCCATCCAGAGGGCGATCGGCCCATCGCGTGCACCATCACCACCTCCTGGAGCC
  GCCTGGAGCTGCGAGACATGCTGATGGCCACCTGCACACTAGAGAACAGCAGCAGCTTCAGCCTGGACCA
  GGGCTGGACCTTGTGTATTCAGGTGCTGACTAGTTCCTCTGCCTTAGACCTGGATGGGACTGGTTCAGCT
  TTCACCTACACCATTCCAGTGGACAGACTGGGTCCAGGCAGCCGGCGGGAAGTGACGTTGCCCCTGGGCC
  CTAGTGAGAGTGGTGTGCTCGACCTGCCTGTGACCATGTCCTGCTGGCTCTTCTACAGCCTCAGGGAGGT
  GGTGGGTGCGGCCCTGGCCCCCTCAGACCCTCTAGAGGCCCCCTACTTGGAGCAGTTCCCTCTCAGCCTT
  CCTAAGCAAGAGGGCGTCTGCCTGCCCCTGTGCAAGCGCACAGTGGACATGCTGCAGTGCCTGCGCTTCG
  CCGGCGCAGCCACACACCCTGCACAGGCCCCCTGTATGCCTGGCCCTGCCTGTGAGCCTGTGGAAACTTT
  CCTGAAGACCTGCCAAGCCCCTGGCAGCGAGCCTACTGGCGCTGCCTCCCTACGGGCCAAGTACCTGCCT
  CCATCCACAGCCTCCATCAGGGTGTCTGCAGGCCTTCTCAGAGCTGCCCTGGAAGACAGTCACTCAGGTT
  TCCACCTGTGCAGCGCCACCCTGCGGTGGCTCCTTGCCGAGAACGCTGCTGTGGATGTTGTGCGGGCCCA
  GACACTGTCTTCCATCCAGGGAATAGCCCCAGATGGAACTGATGTCAACCTCACCGTCCATGAGGTAGCT
  GTGACGGATTTGAGCCCTGCAGGCCCCATTCAGGCTGTGGAGATCCAAGTGGAGAGCTCCTCTCTGGCCA
  ACATGTGTAGGGCGCACCATGCCATCATCAGACGAATACAGACAATGGTCACAGAGCAAGCAGCCCTAGG
  CTCCAGCCCACCTGACCTCCGCATGCAGTATCTACAGCAGATCCATGCCAACCACCAGGAACTGCTCCGT
  GAGGTGCAAGCACTGCGCGACCAGCTGTGCACAGAGGATGAGCTGAGCTCCTGCTCCACGGCCCAGAAGC
  TCCTGCACATTTACAAGCAGCTGCGCAACCCTAGCCTCGTCCTGTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027980
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027980.2, NP_082256.2
• RefSeq Size: 3175 bp
• RefSeq ORF: 2640 bp
• Locus ID: 71885
• UniProt ID: A2ACJ2
• Cytogenetics: 11 E2
• Gene Summary: Plays a role in Fanconi anemia-associated DNA damage response network. Regulates FANCD2 monoubiquitination and the stability of the FA core complex. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed (By similarity).[UniProtKB/Swiss-Prot Function]