Mcm10 (NM_027290) Mouse Untagged Clone

CAT#: MC222381

Mcm10 (untagged) - Mouse minichromosome maintenance deficient 10 (S. cerevisiae) (Mcm10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcm10
• Synonyms: 2410041F14Rik; AU018508; C79164; C330019M07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222381 representing NM_027290
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGGAGGAAGACGACTTGTGTCTCCTGACATCTCTACTGGAAGAGAATGAGGCAGTCTTACCTT
  GCAGCTCAGAAAAGGATAAGTCCTTGTCTCTGGGAGACGGGGACCCTGATGAATTTGATGAGCTCTTTGA
  TGCTGATGGTGATGGTGAGTCTTACACAGAAGAGGCTGGCAGTGGAGAAGAGGGCAAGACTGGAAACCAG
  GAGGAACGTTTGGCCACTCTCTTTGGTGATGTGGAGGACTTAACGGATGATGAAGTTGCTACATCGAAAG
  TTGGAAACAGTGGCCCCCCTCCTGCTCCCAGCCAAGAGAAAACCAGCGAGGAGCTGCAAGATGAATTAAA
  GAAGTTGCAAGAGCAAATGAAATCCTTACAGGAGCAGCTAAAAGCTGCATCAATTAAACAGCCTCCAGGG
  ACAGCACCCTTGCAGGAACCACCTGATTCATCTCTGCAGCCACTGCTTAAGGAGAAGAGAATTCGGAGAA
  TTCAGGAGTCAGCGTGCTTTTCTGCAGAGCTTGATGTCCCCACTTTACCCAAAGCCAAGCGGGTTGCTCG
  GAAGCCAAAGACTCCTGCAGAGTCATCTTCAAGGATGCGTACACCCGCACAGCCACTACAGGTTTCTTCC
  AGTTTTCTAGAGCCTAACCACTCCTCTTCTTCGAGATCAAGCACACCCTCACCTCAAGCTGTTCCTGGAA
  ACAAATGTAGCAGGACAATTAGAAATCAAAACACAGTGTCCCCAGGGAATTCTGGGGACCGGCCCCAGCA
  GGTTTCCCAGGTCTCTGTGGAAGCCTTCTCAGGCCTACGCCTCAGGCGACCTCGAGTCTCCTCCACAGAA
  ATGAGCAGGAAGATGGCAGGCCGGAAACTAATCAGACTGCCTCAGATCAAGGAGAAGATGGCAACTGAGA
  ACCTGGAAGAGACAGACTGGGTGACGTTTGGGGTTATCCTCAGGAAGGTCACTCCACAGAGTGCTACCAG
  TGGGCAAACGTTTAGCATCTGGAAACTGAACGACCTTCATGACCTGACTCAGTGTGTGTCCTTGTTCTTG
  TTTGGAGATGTTCACAAAGATCTCTGGAAGACAGAGCAAGGGACCGTCATAGGCTTGCTCAACGCAAACC
  CCATGAAGCCCAAAGATGGCTTGAAAGAGGTGTGCTTATCTATTGATCATCCTCAAAAAGTCTTAATTAT
  GGGAGAAGCTATGGACCTGGGAGCCTGTAAAGCCAAGAAGAAGAATGGAGAGCCATGTACACAGACAGTT
  AACTTGCATGACTGTGAATACTGCCAGTATCACATCCAGGCCCAGTACAAGAAGCTCAGCGCAAAGAGGA
  CTGATCTGCAGTCCACTTTCTCTGGGGGACGGATTCCGAAGAAGTTCCGCAAAGGCACCAGCCTGAAGGA
  GCGGCTGTGCCAAGATGGTTTCTACTATGGTGGTGTTTCTTCCGAATCCTTCGCAGCGTCCAGGGCGGCG
  GCCATTGCTCCTAAGAAGAAGGTTCAAACCACGCTGACCAATCTGGTTGTCCGGGGCACAAACTCGATCA
  TCCAGGAAACAAAACAAAAGCTTGGTATTCCCCAGAAGAGCTTGTCCTGCTCTGAGGAGTTCAGGGAATT
  GATGGCCCTGCCCACATTTGGAGCCAGAAACTTACAAAAGCATCTAGCCAGAGCCAAGGCTTCAGGGAGT
  TCCAAACCTGCCATCCAGTCCATCTCAGCATCAGCCCTCTTGAAGCAGCAGAAACAACAGATGCTGGAGA
  TGCGGAAGAGGAGATCAGAAGACATACAGAAGCGATTCCTCCAAAGCTCAAGTGAAGTCCAGAGCCCAGC
  AGTGCCGTCTTCCTCTCGACAGGCTGCTGCCCAGTCTCCTCGAACTGGGGCTGAGTTCCCCAGGTTGGAA
  GGAACTGCAACCCCACGGATGCCCAAGCTGGGCAGAGGCATCTCAGAAGGGGACGATGTTCTCTTCTTTG
  ATGATTCACCACCACCAAGACCAAAACTGAGCGCAGCAGCAGAAGCTAAAAAGTTAGCTGCTATTGCAAA
  ATTAAGGGCAAAAGGCCAGATTCTCACAAAAGTAGATCCAAACAATACTGTAAGGAAGCAAATGGATGGC
  CGAGCCATGCTGGGAGTGAAGGAGCGTGTGGAGAATAGCAACACAGTTTCTCCTGAAGAGGAGCTGGAGC
  CTGCCAGGAAAAAAAGGAGAGAGCAGCTTGCCTACTTAGAGTCCGAGGAATTTCAGAAGATTCTAAAAGC
  AAAGTCCAAGCACACAGACATCCTGAAAGAGGCTGAGGCTGAGCTACAGAAATCCTATTTTGAGCCACTG
  GTGAAAAAAGAACAGATGGAAGAAAAGATGAGAGCCACCCGGGAAGTGAAATGCCGCGTGGTGACGTGCA
  GGACGTGCACCTATACCCACTTCAAGCCTCTGGAGACCTGTGTCAGCGAGCAGCATAACCTCCACTGGCA
  CGACGGCGTGAAGAGGTTTTTCAAATGTCCCTGTGGAAACAGGACCATCTCCCTGGACAAGCTCCCAAAC
  AAGCACTGCCGTAACTGTGGCCTCTACAAGTGGGAACGGGATGGAATGCTGAAGGAGAAGACAGGTCCAA
  AGATAGGAGGAGAGACCCTGCTGCCGAGAGGGGAAGAGCACGCCAAGTTTCTGAACAGCCTCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027290
• Insert Size: 2658 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027290.3, NP_081566.2
• RefSeq Size: 3510 bp
• RefSeq ORF: 2658 bp
• Locus ID: 70024
• UniProt ID: Q0VBD2
• Cytogenetics: 2 A1
• Gene Summary: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.