Sema6b (NM_001130456) Mouse Untagged Clone

CAT#: MC222375

Sema6b (untagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sema6b
• Synonyms: Sema; Seman; semaZ; VIb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222375 representing NM_001130456
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACCCCGCGAGTGCCCCCTCCACGCCCGGCCCTGTCGTTTTTCCTGCTGTTGCTCCTGGGGGTCA
  CCTATGGCCTTTTCCCAGAAGAACCACCTCCACTCAGCGTGGCTCCCAGGGACTACTTGAGCCACTATCC
  CGTGTTCGTGGGCAGCGGGCCTGGTCGTCTGACCGCTGCGGAGGGTGCTGAGGACCTCAACATCCAGAGA
  GTGCTACGTGTTAACAGGACACTGTTCATTGGGGACAGAGATAACCTGTACCAAGTAGAACTGGAGCCAT
  CCACTTCCACGGAGCTGCGATATCAGAGGAAGCTGACCTGGCGCTCCAACCCCAGTGACATCGATGTGTG
  TCGAATGAAGGGCAAGCAAGAGGGTGAGTGTCGGAACTTTGTCAAGGTGCTCCTGCTTCGCGATGAGTCC
  ACGCTGTTCGTGTGCGGCTCCAATGCGTTCAATCCCATCTGCGCCAATTACAGTATGGACACACTGCAGC
  TTCTTGGTGACAGCATCAGCGGTATGGCCCGCTGCCCCTATGACCCCAAACATGCCAATGTCGCCCTCTT
  CTCAGATGGGATGCTCTTCACAGCCACGGTGACTGACTTCCTAGCCATCGACGCTGTTATCTACCGCAGC
  CTTGGGGACCGGCCCACACTGCGCACAGTAAAGCACGACTCCAAGTGGTTTAAAGAGCCGTACTTTGTGC
  ATGCAGTGGAGTGGGGAAGCCACGTCTATTTCTTCTTCCGGGAGATCGCCATGGAGTTTAACTACCTGGA
  AAAGGTGGTGGTGTCTCGAGTGGCCCGTGTGTGCAAGAATGACGTGGGCGGCTCGCCACGAGTGCTGGAG
  AAGCAGTGGACTTCCTTCCTGAAGGCCCGGCTCAACTGCTCAGTGCCAGGGGATTCACACTTCTACTTCA
  ACGTCCTGCAGGCTGTGACGGGCGTGGTGAGCCTGGGTGGTCGTCCGGTGATCCTCGCTGTCTTCTCAAC
  TCCTAGCAACAGCATCCCCGGCTCAGCCGTCTGCGCCTTTGACATGAACCAAGTGGCTGCTGTGTTTGAA
  GGCCGCTTCCGGGAACAGAAGTCGCCTGAGTCGATCTGGACACCAGTGCCTGAAGACCAAGTGCCACGGC
  CCAGGCCTGGGTGCTGCGCGGCACCTGGCATGCAGTACAACGCGTCCAGTGCCCTCCCTGACGAGATCCT
  CAACTTTGTAAAGACCCACCCACTGATGGATGAGGCAGTACCTTCCCTGGGTCACTCGCCGTGGATTGTG
  AGAACTCTGATGCGGCACCAGCTGACCCGAGTGGCTGTGGATGTGGGTGCGGGTCCATGGGGCAATCAGA
  CAATAGTCTTCCTTGGCTCCGAGGCTGGCACAGTCCTCAAATTTCTTGTGAAGCCCAACGCCAGTGTCTC
  AGGGACCACAGGGCCCAGCATCTTTTTGGAGGAGTTTGAGACCTACCGGCCAGACAGGTGTGGACGACCC
  AGCAGTGCTGGTGAGTGGGGGCAGCGACTTCTCAGCCTGGAGCTGGATGCAGCCTCAGGGGGCCTGCTGG
  CAGCCTTCCCCCGCTGTGTGGTTCGTGTTCCTGTTGCCCGTTGCCAGCTGTACTCGGGGTGCATGAAGAA
  CTGTATTGGCAGCCAGGATCCATACTGCGGGTGGGCCCCTGATGGCTCCTGCATCTTCCTCAGACCAGGA
  ACTAGTGCCACGTTTGAGCAAGATGTGTCCGGGGCCAGCACCTCTGGCTTAGGTGACTGCACTGGACTCT
  TGCGCGCCAGCCTCTCAGATGACCGCGCAGGGCTGGTGTCGGTGAACCTGCTAGTGACGTCCTCGGTGGC
  AGCGTTCGTGGTGGGTGCCGTGGTGTCCGGCTTCAGTGTAGGCTGGTTCGTGGGTCTCCGCGAGCGGCGG
  GAGCTGGCCCGGCGCAAGGACAAGGAGGCCATTCTGGCGCACGGCGGCAGCGAGGCAGTTCTGAGCGTGA
  GCCGACTAGGCGAGCGCAGGGGGACCGGGCCCGGGGGTCGTGGAGGAGCCGGCGGCGGTCCCGGAGGTCC
  CCCGGAGGCCCTGCTGGCCCCGCTTATGCAGAATGGCTGGACAAAGGCAGCGCTGCTGCATGGTGGCCCT
  CACGACCTGGACACGGGGCTGCTGCCCACCCCAGAGCAGACGCCTCTACCCCAGAAACGCCTGCCCACGC
  CACACCCACACGCCCACGCCCTGGGGTCTCGAGCCTGGGACCACAGCCACGCGCTGCTGTCGGCCTCAGC
  CTCCACCTCCTTGCTCCTACTGGCGCCCGCCCGCGCCTCTGAGCAGCCCCAGGTGCCAGCCGAGCCGGGC
  CCGGAATCTCGCCTCTGCGCCCCCAGATCCTGCCGGGCCTCTCACCCAGGCGACTTCCCGCTCACGCCCC
  ACGCCAGCCCGGACCGCCGGCGGGTGGTGTCCGCACCCACGGGCCCCTTGGACCCTTCGGTGGGCGACGG
  CCTGCCCGGGCCATGGAGCCCACCTGCAACCAGCAGCCTGCGGAGGCCGGGTCCCCACGGGCCCCCCACA
  GCCGCCCTACGGCGCACACACACATTCAACAGCGGCGAGGCTCGGCCCGGGGGTCATCGTCCTCGCCGCC
  ACCCACCCGCGGACTCTACACACTTGCTGCCCTGCGGGACGGGCGAGCGGACTGCACCCCCGGTACCCTA
  G
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_001130456
• Insert Size: 2661 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130456.1, NP_001123928.1
• RefSeq Size: 3736 bp
• RefSeq ORF: 2661 bp
• Locus ID: 20359
• UniProt ID: O54951
• Cytogenetics: 17 D
• Gene Summary: Functions as a cell surface repellent for mossy fibers of developping neurons in the hippocampus where it plays a role in axon guidance (PubMed:20484647). May function through the PLXNA4 receptor expressed by mossy cell axons (PubMed:20484647).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.