Cdh1 (NM_009864) Mouse Untagged Clone

CAT#: MC222365

Cdh1 (untagged) - Mouse cadherin 1 (Cdh1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdh1
• Synonyms: AA960649; ARC-1; E-ca; E-cad; E-cadh; Eca; Ecad; L-C; L-CAM; Um; UVO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222365 representing NM_009864
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCCCGGTGCCGCAGCTTTTCCGCGCTCCTGCTCCTGCTGCAGGTCTCCTCATGGCTTTGCCAGG
  AGCTGGAGCCTGAGTCCTGCAGTCCCGGCTTCAGTTCCGAGGTCTACACCTTCCCGGTGCCGGAGAGGCA
  CCTGGAGAGAGGCCATGTCCTGGGCAGAGTGAGATTTGAAGGATGCACTGGCCGGCCAAGGACAGCCTTC
  TTTTCGGAAGACTCCCGATTCAAAGTGGCGACAGACGGCACCATCACAGTGAAGCGGCATCTAAAGCTCC
  ACAAGCTGGAGACCAGTTTCCTCGTCCGCGCCCGGGACTCCAGTCATAGGGAGCTGTCTACCAAAGTGAC
  GCTGAAGTCCATGGGGCACCACCATCACCGGCACCACCACCGCGACCCTGCCTCTGAATCCAACCCAGAG
  CTGCTCATGTTTCCCAGCGTGTACCCAGGTCTCAGAAGACAGAAACGAGACTGGGTCATCCCTCCCATCA
  GCTGCCCCGAAAATGAAAAGGGCGAATTTCCAAAGAACCTGGTTCAGATCAAATCCAACAGGGACAAAGA
  AACAAAGGTTTTCTACAGCATCACCGGCCAAGGAGCTGACAAACCCCCCGTTGGCGTTTTCATCATTGAG
  AGGGAGACAGGCTGGCTGAAAGTGACACAGCCTCTGGATAGAGAAGCCATTGCCAAGTACATCCTCTATT
  CTCATGCCGTGTCATCAAATGGGGAAGCGGTGGAGGATCCCATGGAGATAGTGATCACAGTGACAGATCA
  GAATGACAACAGGCCAGAGTTTACCCAGCCGGTCTTTGAGGGATTCGTTGCAGAAGGCGCTGTTCCAGGA
  ACCTCCGTGATGAAGGTCTCAGCCACCGATGCAGACGATGACGTCAACACCTACAACGCTGCCATCGCCT
  ACACCATCGTCAGCCAGGATCCTGAGCTGCCTCACAAAAACATGTTCACTGTCAATAGGGACACCGGGGT
  CATCAGTGTGCTCACCTCTGGGCTGGACCGAGAGAGTTACCCTACATACACTCTGGTGGTTCAGGCTGCT
  GACCTTCAAGGTGAAGGCTTGAGCACAACAGCCAAGGCTGTGATCACTGTCAAGGATATTAATGACAACG
  CTCCTGTCTTCAACCCAAGCACGTATCAGGGTCAAGTGCCTGAGAATGAGGTCAATGCCCGGATCGCCAC
  ACTCAAAGTGACCGATGATGATGCCCCCAACACTCCGGCGTGGAAAGCTGTGTACACCGTAGTCAACGAT
  CCTGACCAGCAGTTCGTTGTTGTCACAGACCCCACGACCAATGATGGCATTTTGAAAACAGCCAAGGGCT
  TGGATTTTGAGGCCAAGCAGCAATACATCCTTCATGTGAGAGTGGAGAACGAGGAACCCTTTGAGGGGTC
  TCTTGTCCCTTCCACAGCCACTGTCACTGTGGACGTGGTAGACGTGAATGAAGCCCCCATCTTTATGCCT
  GCGGAGAGGAGAGTCGAAGTGCCCGAAGACTTTGGTGTGGGTCAGGAAATCACATCTTATACCGCTCGAG
  AGCCGGACACGTTCATGGATCAGAAGATCACGTATCGGATTTGGAGGGACACTGCCAACTGGCTGGAGAT
  TAACCCAGAGACTGGTGCCATTTTCACGCGCGCTGAGATGGACAGAGAAGACGCTGAGCATGTGAAGAAC
  AGCACATATGTAGCTCTCATCATCGCCACAGATGATGGTTCACCCATTGCCACTGGCACGGGCACTCTTC
  TCCTGGTCCTGTTAGACGTCAATGACAACGCTCCCATCCCAGAACCTCGAAACATGCAGTTCTGCCAGAG
  GAACCCACAGCCTCATATCATCACCATCTTGGATCCAGACCTTCCCCCCAACACGTCCCCCTTTACTGCT
  GAGCTAACCCATGGGGCCAGCGTCAACTGGACCATTGAGTATAATGACGCAGCTCAAGAATCTCTCATTT
  TGCAACCAAGAAAGGACTTAGAGATTGGCGAATACAAAATCCATCTCAAGCTCGCGGATAACCAGAACAA
  AGACCAGGTGACCACGTTGGACGTCCATGTGTGTGACTGTGAAGGGACGGTCAACAACTGCATGAAGGCG
  GGAATCGTGGCAGCAGGATTGCAAGTTCCTGCCATCCTCGGAATCCTTGGAGGGATCCTCGCCCTGCTGA
  TTCTGATCCTGCTGCTCCTACTGTTTCTACGGAGGAGAACGGTGGTCAAAGAGCCCCTGCTGCCACCAGA
  TGATGATACCCGGGACAATGTGTATTACTATGATGAAGAAGGAGGTGGAGAAGAAGACCAGGACTTTGAT
  TTGAGCCAGCTGCACAGGGGCCTGGATGCCCGACCGGAAGTGACTCGAAATGATGTGGCTCCCACCCTCA
  TGAGCGTGCCCCAGTATCGTCCCCGTCCTGCCAATCCTGATGAAATTGGAAACTTCATCGATGAAAACCT
  GAAGGCAGCCGACAGCGACCCCACGGCACCCCCTTACGACTCTCTGTTGGTGTTCGATTACGAGGGCAGT
  GGTTCTGAAGCCGCTAGCCTGAGCTCACTGAACTCCTCTGAGTCGGATCAGGACCAGGACTACGATTATC
  TGAACGAGTGGGGCAACCGATTCAAGAAGCTGGCGGACATGTACGGCGGTGGTGAGGACGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009864
• Insert Size: 2655 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009864.3, NP_033994.1
• RefSeq Size: 4447 bp
• RefSeq ORF: 2655 bp
• Locus ID: 12550
• UniProt ID: P09803
• Cytogenetics: 8 53.18 cM
• Gene Summary: This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8. [provided by RefSeq, Oct 2015]