Pla2g4e (NM_177845) Mouse Untagged Clone

CAT#: MC222357

Pla2g4e (untagged) - Mouse phospholipase A2, group IVE (Pla2g4e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pla2g4e
• Synonyms: 2310026J01Rik; C230096D22; Pla2epsilon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222357 representing NM_177845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCTATTCCACACTCCGATGAAGCAGACGTGGCTGGGATGACCCACGCCTCAGAAGGCCACCATG
  GCCTGGGGACCAGCATGCTTGTCCCAAAGAACCCACAAGGGGAAGAAGACAGCAAGCTAGGAAGAAACTG
  CAGTGGATTTGAAGATGCACAGGACCCACAGACTGCTGTGCCCTCCTCACCTTTACTTTCCATGGCTTCT
  TGCAGTTCTCAGGAGGGGTCATCTCCATGCCATCTGTTGACAGTGAGGATCATTGGCATGAAAAACGTCC
  GGCAGGCTGATATACTGAGTCAGACAGACTGCTTTGTGACCCTCTGGCTGCCTACTGCCTCTCAGAAGAA
  GCTGAAGACCAGAACCATCTCCAACTGCCTACACCCAGAGTGGGACGAAAGCTTCACCTTTCAGATCCAG
  ACTCAAGTAAAGAATGTGCTAGAGCTGAGCGTCTGTGACGAAGACACCCTGACACAAAATGACCATCTCT
  TGACAGTCCTCTATGACCTCTCTAAGCTTTGCCTCCGGAATAAAACCCATGTGAAGTTCCCACTCAACCC
  AGAGGGCATGGAAGAACTGGAGGTGGAGTTCCTACTCGAAGAGAATTTCTCCTCATCAGAGACCCTCATC
  ACCAACGGCGTGCTGGTGTCTCGCCAAGTCTCTTGCCTGGAGGTTCATGCAGAATCCAGGAGGCCGAGGA
  AGAGGAAGAAAAACAAAGACCTTCTGGTGATGGTGACAGACTCCTTCGAGAACACCCAGCGTGTCCCGCC
  TTGCCAGGAGCCCTGCTACCCCAATTCTGCCTGCTTCCACTACCCCAAGTACTCCCAGCCACAGCTTTAC
  GCAGAGGCGCCTAAGAGCCACTGTAACTTTAGGCTTTGCTGCTGCGGAACACACAGGAATGACCCTGTCT
  GCCAGCCCCTCAATTGCCTTTCTGATGGCCAGGTGACAACCCTGCCTGTGGGAGAGAACTATGAGCTACA
  CATGAAGTCCTCACCCTGCTCTGACACACTGGATGTGCGGCTTGGATTCAGCCTGTGCCAGGAAGAGGTG
  GAGTTTGTGCAGAAGCGGAAGATGGTGGTGGCCAAGACACTAAGTCAGATGCTGCAGCTGGAGGAAGGCC
  TGCATGAGGATGAGGTACCGATAATAGCCATCATGGCCACAGGAGGTGGCACAAGGTCTATGGTCTCCTT
  GTATGGCCACCTGCTGGGGTTGCAGAAGCTGAACTTTCTGGACGCTTCTACTTACATCACCGGCTTGTCA
  GGTGCAACCTGGACTATGGCTACCTTGTACAGTGATCCTGAGTGGTCCTCCAAAAACCTGGAGACTGTTG
  TCTTTGAGGCCCGGAGACATGTTGTCAAAGACAAGATGCCTGCCCTGTTCCCAGATCAGCTCTACAAATG
  GCGAGAGGACCTCCAAAAGCATAGCCAGGAGGGCTATAAGACCACGTTTACAGACTTTTGGGGCAAGCTG
  ATCGAGTACAGTCTGGGAGATAAAAAAAACGAATGCAAGCTGTCAGATCAGCGAGCTGCTCTGTGCAGGG
  GACAGAACCCTCTGCCCATCTACCTCACCATCAATGTCAAGGATGATGTAAGCAACCAGGATTTCAGAGA
  ATGGTTCGAGTTCTCCCCCTACGAGGTGGGCATGCAGAAGTACGGAGCCTTCATCCCCAGCGAGTTATTT
  GGCTCCGAGTTCTTCATGGGGCGGCTGATGAAGAGGATTCCTGAGCCGGAGATGTGCTACATGCTAGGGT
  TGTGGAGTAGCATCTTTTCCCTGAACCTGCTTGATGCCTGGAATTTGTCTCACACCTCAGAGGAGTTTTT
  CTATAGGTGGACAAGGGAGAGACTGCATGACATCGAAGATGATCCCATCCTGCCTGAAATCCCTAGGTGT
  GACGATAACCCCCTAGAGACCACAGTAGTGATCCCAACGACATGGCTGTCCAACACCTTCCGAGAAATCC
  TCACACGCAGGCCCTTCGTGTCTGAGTTCCACAACTTCCTGTACGGGATGCAGCTGCATACTGACTACTT
  ACAGAACAGGCAGTTCTCTATGTGGAAAGACACAGTACTGGACACCTTCCCAAACCAGCTGACACAGTTT
  GCAAAACACCTGAACCTGCTGGACACTGCGTTCTTTGTCAACTCCAGCTACGCACCCCTCCTTAGGCCAG
  AGAGAAAAGTCGACCTTATCATCCACCTCAATTACTGCGCAGGATCCCAGACAAAGCCCCTGAAACAAAC
  CTGTGAGTACTGTACCGAGCAGAAGATCCCCTTCCCCAGCTTCTCCATCCTGGAAGATGACAACAGTCTC
  AAGGAGTGCTACGTGATGGAGAATCCCCAGGAGCCCGACGCCCCCATCGTGGCTTACTTCCCACTCATCA
  GTGACACCTTCCAGAAGTACAAGGCTCCAGGTGTAGAGCGAAGTCCTGACGAGCTGGAACTGGGCCAGCT
  GAACATCTATGGACCAAAGTCTCCCTATGCCACCAAGGAGCTGACGTACACAGAGGCCGCCTTCGACAAG
  CTGGTGAAGCTCTCAGAATATAATATCCTCAATAACAGAGATAAGCTCATTCAGGCCTTGAGACTAGCAA
  TGGAGAAGAAACGCATGAGGAGCCAGTGTCCCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177845
• Insert Size: 2628 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177845.4, NP_808513.2
• RefSeq Size: 4477 bp
• RefSeq ORF: 2628 bp
• Locus ID: 329502
• UniProt ID: Q50L42
• Cytogenetics: 2 E5
• Gene Summary: Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).