Spef2 (NM_177123) Mouse Untagged Clone

CAT#: MC222355

Spef2 (untagged) - Mouse sperm flagellar 2 (Spef2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spef2
• Synonyms: C230086A09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222355 representing NM_177123
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAAATCCTGTGCCAGTGGCTCAACCAGGAGTTGAGGGTGTCCCGGACCGTGAGTCCCAAGTCAT
  TTGCAAAGGCATTTTCTAATGGCTATCTAATTGGAGAAGTTCTGTTCAAGTTTGAACTTCAGAGTGATTT
  TGCAGAATTTTCAGAGAGCAGGGGTTCAACAGCCAAGCTTAACAATTTCTCCCGCTTGCAACCAACACTT
  CACCTGCTGGGTTTGCAGTTTGATCAGAATGTGGCTCAAAGCATCATCACAGAGAAGCCTGGGGCAGCAA
  CCAAACTTCTGTATCAGTTGTACATTGCTCTTCAGAAAAAGAAGAAAACAGGGCTGACTGGACTAGAGAT
  ACAAACCATGCAACCCCAGACAAACTTGAGGCTTCAGACCCTCAAAAGTGAGGCTTTTCGAGAGCAACGC
  TTAAACAGAAGACGACAAAATGAGATAATGGCCAAAATCCAGGCAGCCATTATACAGATCCCCAAGCCTG
  AATCCAACCGTACTTTGAAAGCTATTGAGGCTCAAAAACTGATGAAAAAGAAAAAGGAGGCAGAGGACGT
  GGCCAATGAGATTAAGAAATTTGAAGCACTAATTAAAAAGGATCTCCAAATTAAAGAAAGTGTATCCAAG
  ACTTCTTTAGAAACAACAGACCAGACAACTGCAGAACTGCTCAACACTTATTCAGATGATGACTACATTA
  AAAAGATCCAGAAACGCCTAGAAGAAGACGCTTTCGCACGCGAGCAAAGGGAAAAAAGACGACGCAGATT
  GTTGATGGATCAGCTAATGGCCCATGAAGCACAGGAGGAGGCCTATCGGGAAGAGCAGCTCATTCACCGG
  CTCATGCGCCAGTCCCAGCAGGAGCGCAGGATTGCTGTGCAGCTCATGCATGTTCGCCACGAGAAGGAAG
  TTTTGTGGCAAAATAGAATTTTCCGAGAGAAACAATTTGAAGAAAGACGACTTAAGGATTTCCAGGATGC
  TCTTGATAGAGAAGCGGCTCTTGCCAAACAAGCCAAGATTGATTTTGCAGAACAAACCTTGAGGGAGAAG
  GAAATTCATGAAAAGATCTCTGTGGAAAGAGCCCAACAGCGTTATAAGAAGCATTATGGGATATGTGCAG
  AGATTTTGGATCAAATGCTAGATTTGTGCACGAAGGTGGCAGACTATAGGTTGCTGACAAATAATCTTAT
  TCCACATAAGATGATGCACGATTGGAAAGAACTGTTTTTTAGTGGAATTCCCATATATGAACAGACTTCT
  CTTACACATGGACAAACAGAACCCACTGAGGATCACCGTGCAGAAGTGAAGAAAAGGAACTTACTCGATA
  GCAAAGATTATGAAGATTATAAGAACATGGTTGGAGAGTGGGCCTTACCAGAGGACATGGTTAATAGTTC
  ACCACCCTCCAACAATAGCATTTTGGGTCACGTGCTTCTGAGATTAATTGAAAAAGCTGATCCATCTGCA
  AGTAATGCTGAAGCCACTGAATTACCTTCACTTGCGGTCAAGGGTTGTATACTGGGGAAGACACTCAGTG
  GAAAAACTACTGTTCTAAAGTCTCTACAAAATGACTTTCCTGTTCATGTGCTTTCTATTGATACTCTTGT
  CCAAGAGGCTATCCAGGCGTTTCATGAAAGACAAAAAAGCGGTAAGACACCACCAACTCAGGAAGATGAT
  AAAAGAGATCCTGTAGTGAACCAGGAAAAAGTGAGCAAAACTCAGGATAAAAATGTGTTAGCAGTTAGTC
  CAGTTCCAGGGGACAGAACATCCCAAAAAGAAGGTGTCAAGATTAATGAATTTGAACAGTTCAGATCAAG
  TGATAGCTTTTTATCACTTAGCATGCGAGCTCAACTTGGTGCAAAGTCAGAGTTGATGCTTAGAAGAGGA
  AAGAGCATTCCAGACATACTTCTGGTCAGCATCCTGGTAAATGCTATAAAGGAGATACCTGTGGACCAAA
  GCTGGGTTCTTGATGGATTTCCAATAACATTAAATCAAGCCAAACTCCTGGAGGAAGCACTTACAGGGTA
  CAAAAGAAAATTCCTACAGTTAAAGAAGAAGAAAGAACAAATGCCCACATTGGCCCTAGACCCCTCAACT
  TCCACAGAGGTCTCTCTTCTCCCATCTGCACTTGATTTTGTCATATTGCTAGATATTTCCGATAACTCAT
  CGTTGGCTCGAACTAATGATATCATAGCTAAAGAAATCTCACATGAAATTTCTCATGAAAATGTTGGCCG
  TCCTGGAACTGGTACAAGCCAAGATAATAAGAGCGAGGACCGGAACTTAAGAGACCACATACAGCACAGA
  ATTGTTGGCTTCTTGGACAACTGGCCACTGTTGGAAGAGTGGTTTACACAGCCAAAAAACATTCTGACGA
  AAGTCAATGCTGAAATAGATGAAGCGCTCTTATGCCAAAAAGTAAAAGAAATTTTTGCTACTGAAACAGT
  AAACAAAAAGATCAAAGTTGAAAAGACACTGGAAGAAAAGGAAACTGAGAAAAAGGCAGGAGCTCCCCCG
  GCTGAGCCTCCCGCTATGAGCCCTCCCCTTTCTTCTGAAGCAGAAAAAGACAAGGAGCTTCATCAGGCAA
  AAACTCCTGGGAAAGGAAAGACACAATCAGGTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177123
• Insert Size: 2628 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177123.4, NP_796097.2
• RefSeq Size: 4247 bp
• RefSeq ORF: 2628 bp
• Locus ID: 320277
• UniProt ID: Q8C9J3
• Cytogenetics: 15 A1
• Gene Summary: Required for correct axoneme development in spermatozoa (PubMed:21715716, PubMed:28619825). Important for normal development of the manchette and sperm head morphology (PubMed:28619825). Essential for male fertility (PubMed:21715716, PubMed:28619825). Plays a role in localization of the intraflagellar transport protein IFT20 to the manchette, suggesting function as an adapter for dynein-mediated protein transport during spermatogenesis. Also plays a role in bone growth where it seems to be required for normal osteoblast differentiation (PubMed:28619825).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks numerous 3' exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.