Gnas (NM_201617) Mouse Untagged Clone

CAT#: MC222309

Gnas (untagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnas
• Synonyms: 5530400H20Rik; A930027G11Rik; C130027O20Rik; G; Ga; Galphas; Gn; Gnas1; Gnasxl; GPSA; Gs-; Gs-alpha; Gsa; GSP; N; Nes; Nesp; Nesp55; Nespl; Oed; Oed-Sml; Oedsml; P; P1; P2; P3; PHP1A; PHP1B; POH; SCG; SCG6; XL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222309 representing NM_201617
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATGTTCAACTGCCTCCACGGCAATAATATGTCAGGACAACACGATATCCCCCCTGAAGTCGGGG
  AGCAGCCCGAGCAAGAACCTTTGGAAGCCCCAGGGGCAGCTGCCCCCGGTGCTGGGGCTGGCCCAGCCGA
  AGAAATGGCGACCGAACCGGACTCCGAACCGTCTAACAATGAGCCCGTCCCCGACGAGACTGGCAGTGAG
  ATCAGTGGACCCCCAGAAGACTCCAAATCTGACATCCAAAGCCCCTGCCAGGCCTTCGAGGAAGTCCGAG
  TGGGTGGAGACTACAGCCCACCTCCGGAGGAAGCCATGCCATTCGAGACACAACAGCCCAGCCTGGGAGA
  TTTCTGGCCCACCCTGGAGCAGCCAGGACCATCTGGGACCCCATCAGGCCTCCAAGCCTTCAACCCAGCG
  ATTTTGGAGCCCGGGACCCCCACTGGCGCGAGCCCAGGCCTGGGAGCCTATACCCCCCCACCAGAAGAAG
  CTATGCCATTTGAGTTCAACGAGCCTGCCCAGGGAGACCATAGCCAGCCTCCCTTGCAAGTCCCAGACCT
  TGCGCCAGGAGGTCCGGAAGCATTGGTCCCCAGAGCTCTTCCCGCGGAGCCCGGGAACATCAGATTTGAA
  AACGCTGGCTTCCGAGAAGACTACAGCCCTCCCCCTGAAGAATCTGTGCCATTTCAGGTCGGTGGAGAAG
  AATTCGGGGGCGATAGCCCACCCCCAGGACTCCCGCGAGTCATCCCACAAATCGGCATTGGCGGCGAGTT
  CCCGACAGTCGCGGTCCCGAGTGCGCTCTGCCTCGCTCCCGCCGAGAACGCGCCTCCCCTCTGGGTCCGA
  GGCGCCATTGACAGACCATTCCGCGAGGCTGTCAGATCTCCTCCTAACTTCGCATGCGACAGCCCCCCGA
  TGGAGATCACCAGACCCCTGCTTGAGATTGGCAGAGCCTCCATTGGGGTCGACGACGACACCGCTGTCAA
  TATGGACAGCCCCCCAATCGCAAGTGATGGCCCGCCCATCGAAGTCTCGGGAGCCCCAGATAAGAGCGAG
  TGCGCAGAGAGACCCCCAGTTGAGCGAGAAGCAGCCGAGATGGAAGGAAGCCCTACCACCGCCACTGCGG
  TGGAAGGAAAAGTCCCCTCTCCGGAGAGAGGGGACGGATCTTCCACCCAGCCTGAAGCAATGGATGCCAA
  GCCAGCCCCTGCTGCCCAAGCCGTCTCTACCGGATCTGATGCTGGAGCTCCTACGGATTCCGCGATGCTC
  ACAGATAGCCAGAGCGATGCCGGAGAAGACGGGACAGCCCCAGGAACGCCTTCAGATCTCCAGTCGGATC
  CTGAAGAACTCGAAGAAGCCCCAGCTGTCCGCGCCGATCCTGACGGAGGGGCAGCCCCAGTCGCCCCAGC
  CACTCCTGCCGAGTCCGAGTCTGAAGGCAGCAGAGATCCAGCCGCCGAGCCAGCCTCCGAGGCAGTCCCT
  GCCACCACGGCCGAGTCTGCCTCCGGGGCAGCCCCTGTCACCCAGGTGGAGCCCGCAGCCGCGGCAGTCT
  CTGCCACCCTGGCGGAGCCTGCCGCCCGGGCAGCCCCTATCACCCCCAAGGAGCCCACTACCCGGGCAGT
  CCCCTCTGCTAGAGCCCATCCGGCCGCTGGAGCAGTCCCTGGCGCCCCAGCAATGTCAGCCTCTGCTAGG
  GCAGCTGCCGCTAGGGCAGCCTATGCAGGTCCACTGGTCTGGGGAGCCAGGTCACTCTCAGCTACTCCCG
  CCGCTCGGGCATCCCTTCCTGCCCGCGCAGCAGCTGCCGCCCGGGCAGCCTCTGCTGCCCGCGCAGTCGC
  TGCTGGCCGGTCAGCCTCTGCCGCCCCCAGCAGGGCCCATCTTAGACCCCCCAGCCCCGAGATCCAGGTT
  GCTGACCCGCCTACTCCGCGGCCTCCTCCGCGGCCGACTGCCTGGCCTGACAAGTACGAGCGGGGCCGAA
  GCTGCTGCAGGTACGAGGCATCGTCTGGCATCTGCGAGATCGAGTCCTCCAGTGATGAGTCGGAAGAAGG
  GGCCACCGGCTGCTTCCAGTGGCTTCTGCGGCGAAACCGCCGCCCTGGCCTGCCCCGGAGCCACACGGTC
  GGGAGCAACCCAGTCCGCAACTTCTTCACCCGAGCCTTCGGAAGCTGCTTCGGTCTATCCGAGTGTACCC
  GATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCCTATGGAGGAGAGGCGCAAACAGATGCGCAAAGA
  AGCCATTGAGATGCGAGAGCAGAAGCGCGCAGATAAGAAACGCAGCAAGCTCATCGACAAGCAACTGGAG
  GAGGAGAAGATGGACTACATGTGTACACACCGCCTGCTGCTTCTAGGGAGAAAAGTGGTGCCGAGCGACA
  CTGAGGGTCGTTACCGGCCGGAGGCCAGCGCCAGCGCCAGCGATCGTAGGCTGGACAGGCGGGGGCGTGA
  GGTGAGCCCAGAACTGCTGGGGTGGGCCCTTCGGGGCTCCCCCGGCTCGATTGTTCGTGACCGCGGTGGG
  CTAGGGCCATCCGGGTGCGCGCCCCCGCCTCGCCTGGCACGGCTGCTTCGACTCAGACAGCTTGTTGTTG
  GTGTGTGTTGGTGTCCATTTTCTGTGTTCGCCTGTGCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_201617
• Insert Size: 2631 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201617.2, NP_963911.1
• RefSeq Size: 2734 bp
• RefSeq ORF: 2631 bp
• Locus ID: 14683
• UniProt ID: Q6R0H7
• Cytogenetics: 2 97.89 cM
• Gene Summary: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2015]
  Transcript Variant: This variant (6) is paternally expressed. Its 5' exon overlaps with variant 1 and none of its sequence overlaps with variant 7. This variant encodes isoform XXLb1, which has a distinct and shorter C-terminus, compared to isoform XLas. This variant is based on data in PMID:15148396. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.