Srrt (NM_001109909) Mouse Untagged Clone

CAT#: MC222279

Srrt (untagged) - Mouse serrate RNA effector molecule homolog (Arabidopsis) (Srrt), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Srrt
• Synonyms: 2810019G02Rik; Ars2; Asr2; ASR2A; ASR2B; ASR2C; ASR2D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222279 representing NM_001109909
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACAGTGATGATGAATACGACCGAAGACGCAGGGACAAATTTCGAAGAGAGCGCAGCGATTATG
  ACCGTTCCCGGGAAAGGGATGAAAGACGGCGAGGGGACGATTGGAATGACCGAGAGTGGGACCGTGGCCG
  GGAGCGCCGCAGTCGGGGTGAATATCGAGACTACGACAGGAACCGAAGGGAGCGCTTCTCTCCCCCTCGA
  CACGAACTAAGCCCCCCCCAGAAGCGCATGCGGAGAGACTGGGATGAGCACAGCTCTGACCCATACCACA
  GTGGCTATGACATGCCCTATGCTGGGGGGGGTGGGGGACCAACTTACGGCCCCCCTCAGCCCTGGGGCCA
  CCCAGACGTCCACATCATGCAGCACCATGTCCTGCCCATCCAGGCCAGGCTGGGCAGCATCGCAGAGATT
  GACTTGGGGGTGCCACCGCCCATAATGAAGTCCTTCAAAGAGTTCCTCCTGTCTCTGGATGACTCTGTGG
  ATGAGACAGAGGCAGTTAAACGCTACAATGACTACAAGCTGGACTTCCGAAGGCAGCAGATGCAGGACTT
  TTTCCTGGCTCACAAAGACGAGGAGTGGTTCCGATCTAAGTACCACCCTGATGAGGTGGGAAAGCGTCGG
  CAGGAGGCCCGGGGGGCCCTGCAGAACCGGCTGAAGGTGTTCCTGTCCCTCATGGAGAGTGGCTGGTTTG
  ATAACCTTCTCTTGGACATAGACAAAGCTGATGCCATTGTCAAGATGCTAGATGCAGCTGTCATTAAGAT
  GGAAGGTGGCACAGAGAACGATCTCCGAATTTTGGAGCAGGAGGAGGAGGAGGAACAGGCAGGCAAGACT
  GGGGAGGCCAGCAAGAAAGAGGAGGCCCGTGCTGGACCAGCCCTGGGAGAAGGAGAGCGCAAAGCCAATG
  ATAAGGATGAGAAGAAAGAAGATGGAAAACAGGCTGAGAATGACAGTTCCAACGATGACAAAACTAAAAA
  ATCTGAGGGTGATGGGGACAAGGAGGAGAAGAAAGAAGAGGCTGAGAAGGAAGCCAAAAAGAGCAAGAAG
  CGGAACAGGAAGCAGAGTGGCGATGACAGCTTCGATGAGGGCAGTGTGTCCGAGTCTGAGTCCGAGTCTG
  AGGGTGGCCAGGCCGAGGAGGAGAAGGAGGAGGCCGAAGAAGCACTTAAAGAAAAGGAGAAGCCCAAAGA
  GGAGGAGAAGGAGAAGCCTAAGGATGCTGCAGGGTTGGAGTGTAAGCCCCGGCCCTTGCATAAGACTTGC
  TCTCTCTTCATGCGCAACATCGCACCCAACATTTCAAGGGCAGAGATCATTTCTCTTTGTAAACGATACC
  CAGGCTTTATGCGAGTGGCACTGTCAGAGCCCCAGCCAGAGAGGAGGTTTTTTCGCCGTGGCTGGGTGAC
  TTTTGACCGCAGTGTTAACATTAAGGAGATCTGTTGGAACCTGCAGAACATTCGGCTCCGGGAGTGTGAA
  CTGAGTCCCGGTGTGAACAGAGACCTGACCCGTCGTGTCCGCAACATAAATGGCATTACACAGCACAAGC
  AGATAGTGCGCAATGACATCAAGTTGGCAGCCAAGCTAATCCACACACTGGATGACAGGACCCAGCTCTG
  GGCCTCTGAGCCTGGGACGCCTCCTGTGCCCACAAGCCTCCCCTCGCAAAACCCCATCCTGAAGAACATC
  ACTGACTACCTGATTGAGGAAGTGAGTGCGGAGGAGGAGGAGCTTCTGGGGAGCAGTGGGGGACCCCCTC
  CTGAGGAGCCTCCCAAGGAGGGCAACCCAGCCGAGATCAACGTGGAGAGAGATGAGAAGCTGATCAAGGT
  CTTGGATAAACTTCTTCTCTATTTGCGTATTGTGCATTCTCTGGATTATTATAACACCTGTGAGTACCCT
  AATGAAGACGAGATGCCCAACCGCTGTGGCATAATCCACGTTCGGGGGCCCATGCCTCCCAACCGAATTA
  GTCACGGAGAAGTGCTGGAGTGGCAGAAGACATTTGAGGAGAAACTGACTCCACTGTTGAGTGTGCGTGA
  ATCCCTTTCTGAGGAAGAGGCCCAGAAGATGGGTCGAAAAGACCCAGAGCAGGAAGTGGAGAAGTTTGTC
  ACCTCCAACACGCAGGAACTGGGCAAGGATAAGTGGCTATGTCCTCTCAGTGGCAAGAAATTCAAGGGCC
  CGGAGTTTGTGCGCAAGCATATCTTCAATAAGCATGCCGAGAAGATCGAGGAGGTGAAGAAGGAGGTGGC
  GTTCTTCAATAACTTTCTCACAGACGCCAAGCGCCCAGCTTTGCCTGAGATCAAGCCAGCTCAGCCACCT
  GGCCCTGCCCAGATACTCCCCCCAGGCCTGACCCCAGGACTTCCCTACCCACATCAGACGCCACAGGGCT
  TGATGCCATATGGTCAGCCCCGGCCTCCCATCTTGGGCTATGGAGTCCCAACAGGAGGGCCTCCATACCC
  CCATGCTCCATATGGTGCCGGCCGTGGGAACTATGATGCTTTTCGAGGCCAAGGCGGTTATCCTGGGAAA
  CCTCGGAACAGGATGGTTCGAGGAGACCCAAGGGCCATAGTGGAGTATCGGGACCTGGATGCCCCGGATG
  ATGTTGACTTCTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001109909
• Insert Size: 2607 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109909.1, NP_001103379.1
• RefSeq Size: 3018 bp
• RefSeq ORF: 2607 bp
• Locus ID: 83701
• UniProt ID: Q99MR6
• Cytogenetics: 5 G2
• Gene Summary: Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different acceptor splice site at the penultimate exon compared to transcript variant 1, resulting in a shorter isoform (2) missing a 7 aa segment, compared to isoform 1.