Trim71 (NM_001042503) Mouse Untagged Clone

CAT#: MC222236

Trim71 (untagged) - Mouse tripartite motif-containing 71 (Trim71), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim71
• Synonyms: 636931; 2610206G21Rik; AL022943; Gm1127; lin-41; Lin41; mlin-41; mLin41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222236 representing NM_001042503
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGTTCCCGGAGACCGACTTCCAGATCTGCCTGCTGTGCAAGGAGATGTGCGGCTCGCCCGCGC
  CGCTCTCCTCCAACTCGTCCGCCTCGTCGTCGTCCTCGCAGACTTCCACGTCGTCGGCGGGCGGCGGCGG
  CCCCGGGGCGGCGGCGCGGCGTCTTCACGTCTTGCCCTGCCTGCACGCCTTCTGCCGGCCGTGCCTCGAG
  GCGCACCGGCTGCCCGCTCCCGGAGGCGCGGGCCCCGCCGAGGCGCTGAAGCTGCGCTGCCCCGTATGTG
  ACCAGAAGGTGGTCCTGGCCGAGGCGGCGGGCATGGACGCGCTGCCATCCTCCGCCTTCCTGCTCAGCAA
  CCTGCTGGACGCCGTGGTGGCCACCGCCGAAGAGCCGCCGCCCAAGAACGGGCGCGCGGGCGGCGGCCCC
  GGGGGCGCGGGCGGCCACAGCAACCACCGGCACCATGCGCACCACCCGGCCCAGCGCGCCGCCGCTCCAG
  CGCCGCAGCCGCCGCCCGGCCCGGCCGCCTCCCCCGGGTCGCTGCTGATGCGCCGGCCGCACGGCTGCAG
  CTCCTGCGACGAGGGCAACGCCGCCTCGTCGCGCTGCCTCGACTGCCAGGAACACCTGTGCGACAACTGT
  GTCCGCGCGCACCAGCGCGTCCGCCTCACCAAGGACCATTACATCGAGCGCGGCCCTCCGGGGCCTGCGG
  CCGCCTCCGCGGCACAGCAGCTGGGGCTCGGGCCGCCCTTCGCGGGCGCGCCCTTCTCCATTCTCTCGGT
  GTTCCCGGAGCGCCTCGGCTTCTGCCAGCACCACGACGATGAGGTCCTGCATCTCTACTGTGACACCTGC
  TCTGTCCCCATCTGCAGAGAGTGCACGCTGGGCCGCCACGGTGGCCACAGCTTCGCCTACCTCCAGGATG
  CCCTGCAGGACTCTCGGGCACTCACCATCCAGCTGCTGGCTGATGCCCAGCAGGGCCGCCAGGCTCTCCA
  GCTGAGCATCGAGCAGGCCCAGACTGTGGCAGAACAGGTGGAGATGAAGGCGAAGGTGGTCCAGTCTGAG
  GTCAAGGCCGTCACAGCTCGCCACAAGAAGGCCCTGGAGGATCGGGAGTGTGAGCTGTTGTGGAAGGTGG
  AGAAGATCCGGCAGGTGAAGGCGAAGTCTCTGTTCCTGCAGGTGGAGAAGCTGCGGCAGAGTCTCAGCAA
  GCTGGAGAGCACCATCAGCGCCGTGCAGCAGGTCCTGGAAGAGGGCCGCGCCCTGGACATCCTGCTGGCC
  CGAGACCGGATGCTGGCCCAGGTGCAGGAGCTCAAGACCATCCGAGGCCTCCTGCAGCCCCAAGAAGATG
  ACCGCATTATGTTCACGCCCCCTGACCAGGCCCTGTACCTCGCCCTCAAGTCTATTGGCTTTGTCAGCAG
  CGGGGCCTTCGCCCCGCTCACGAAGGCTACAGGAGATGGCATCAAGCGTGCCCTTCAGGGGAAGGTGGCC
  TCTTTCACTGTCATGGGCTACGACCACGATGGTGAGCCCCGCCATTCGGGAGGTGACCTGATGTCAGTTG
  TGGTCCTCGGCCCCGACGGAAACCTGTTTGGTGCAGAAGTGAGCGACCAGCAGAATGGTACTTACATAGT
  GAGCTACAGGCCCCAGCTTGAGGGTGAGCACTTGGTGTCGGTGACATTGTACAATCAGCATATAGAGAAC
  AGCCCCTTCAAGGTCGTGGTCAAGTCGGGCCGCAGCTACGTGGGCATCGGGCTCCCCGGGCTGAGCTTCG
  GCAGTGAAGGTGACGGGGAGGGCAAGCTCTGCAGGCCCTGGGGCGTGAGTGTTGATAAGGAGGGTTTCAT
  CATAGTGGCTGACCGAAGCAATAACAGAATCCAGGTGTTTAAGCCCTGTGGTTCCTTCCACCACAAGTTT
  GGTACCCTTGGGTCTCGTCCTGGGCAGTTTGACCGACCGGCTGGGGTGGCTTGTGACGCCTCCCGCAGGA
  TCATAGTGGCCGACAAAGACAATCATCGCATTCAGATCTTCACCTTCGAGGGCCAGTTCCTTCTTAAGTT
  TGGTGAGAAAGGCACCAAGAACGGGCAGTTTAACTACCCGTGGGATGTAGCAGTGAATTCTGAGGGCAAG
  ATCCTCGTTTCAGACACCCGGAATCATCGCATCCAGTTGTTCGGACCGGACGGGGTCTTCCTGAACAAGT
  ACGGCTTCGAGGGATCTCTCTGGAAGCACTTCGACTCTCCACGGGGCGTGGCTTTCAACAATGAAGGTCA
  CCTGGTAGTCACCGACTTCAACAACCACCGGCTTCTGGTTATTCACCCTGATTGCCAATCTGCCCGCTTC
  TTGGGTTCGGAGGGCTCGGGCAATGGGCAGTTCCTGCGACCACAGGGTGTGGCTGTGGACCAGGAAGGGA
  GAATCATCGTAGCCGATTCCAGAAATCACCGCGTGCAGATGTTTGAGGCCAATGGCAGCTTCCTGTGCAA
  GTTTGGTGCTCAAGGGAGTGGCTTCGGGCAGATGGACCGCCCCTCAGGTATCGCGGTCACCCCGGATGGA
  TTGATCGTCGTGGTGGACTTTGGCAACAATCGAATCCTCATCTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042503
• Insert Size: 2568 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042503.2, NP_001035968.1
• RefSeq Size: 5197 bp
• RefSeq ORF: 2568 bp
• Locus ID: 636931
• UniProt ID: Q1PSW8
• Cytogenetics: 9 F3
• Gene Summary: E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (PubMed:19898466). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression (PubMed:22735451). In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism (PubMed:23125361). Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (PubMed:22735451). Specific regulator of miRNA biogenesis. miRNA Binds MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression (By similarity).[UniProtKB/Swiss-Prot Function]