Epb41 (NM_183428) Mouse Untagged Clone

CAT#: MC222203

Epb4.1 (untagged) - Mouse erythrocyte protein band 4.1 (Epb4.1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epb41
• Synonyms: 4.1R; AI415518; D4Ertd442e; Elp-1; Elp1; Epb4.1; mKIAA4056
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222203 representing NM_183428
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACAGAGAAGAGTTTAGCGGCTGAAGCTGAGAATTCTCAGCACCAGCAACAGAAGGAAGAAGGAG
  AGGGAGCCACAAACTCAGGCCAACAAGAAACTCAGCTGGAGGAGGCCTCCCAAGCGGCCGCAGCTGAGGG
  CAGCGATCAGGGTGAGCAGAAGCTGAAAGCATCCAATGGGGACACTCCAACACATGAAGACTTGACCAAG
  AACAAGGAACGGACATCAGAAAGCAGAGGCCTGTCACGACTGCTCTCCTCATTCCTCAAAAGGCCCAAGT
  CGCAGGTCTCTGAAGAAGAAGGCAGAGAAGTAGAATCAGAGAAAGAGAAAGGTGAAGGGGGTCAGAAGGA
  GATAGAACTTGGAAACAGCCTTGATGAAGACATCATTTTAAAGGCCCCCATTGCAGCTCCTGAACCTGAG
  CTCAAAACAGACCCATCTTTGGATCTTCATTCATTAAGCAGTATAGAGACACAGCCAGCTCAGGAAGAAC
  ACAGAGAGGACCCAGATTCTGAAACGAAGGAAGGAGAAGGGATTGAAGAGTGCTCTGGAACAGAGGTGAA
  AGAGGACCCAGAGTCAAGAGCAGAGAGAGAACCCGAAGCCTCCCAGAAGCCCGTCAGAAGACACAGAAAC
  ATGCACTGTAAGGTCTCCTTGTTGGATGACACGGTCTACGAATGTGTTGTAGAGAAACATGCTAAGGGAC
  AAGACTTGCTGAAGCGAGTGTGCGAGCACCTCAACCTTTTGGAAGAAGACTACTTTGGTTTAGCCCTGTG
  GGACAGCGCAACCTCTAAGACATGGCTGGATTCTGCCAAAGAAATAAAAAAGCAGGTTCGAGGTGTTCCT
  TGGAATTTCACATTTAATGTGAAGTTTTATCCACCCGACCCAGCACAATTAACAGAAGACATAACAAGAT
  ACTATTTATGTCTTCAGCTTCGGCAGGACATTGTTGCTGGACGTCTGCCCTGTTCCTTTGCAACTTTAGC
  CCTACTCGGGTCTTACACGATCCAGTCTGAGCTGGGAGACTATGACCCAGAACTGCACGGCATGGATTAT
  GTTAGTGATTTTAAACTGGCTCCAAATCAGACCAAGGAACTTGAAGAAAAGGTCATGGAATTGCATAAAT
  CATACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAATTTCTTGAGAATGCCAAAAAGTTGTCCATGTA
  TGGAGTTGATCTTCACAAAGCAAAGGACTTGGAGGGAGTGGACATTATTCTCGGCGTCTGCTCCAGCGGC
  CTTCTGGTTTACAAAGACAAGTTGAGAATTAACCGCTTTCCTTGGCCCAAAGTGCTAAAGATTTCTTACA
  AACGCAGCAGCTTCTTCATCAAGATCCGGCCTGGAGAGCAAGAACATTATGAAAGTACCATCGGCTTCAA
  GCTCCCCAGTTATCGAGCGGCCAAGAAACTATGGAAGGTCTGTGTGGAGCATCACACGTTCTTCAGACTC
  ACCTCTACAGACACCATCCCCAAAAGCAAGTTTCTTGCCCTGGGATCCAAATTCCGATACAGTGGCCGGA
  CTCAAGCTCAGACCAGGCAAGCCAGTGCCCTGATTGACAGGCCTGCTCCACACTTTGAGCGGACAGCAAG
  CAAGCGGGCGTCCAGGAGCCTCGATGGAGCAGCAGCTGCTGAGTCCACAGACCGAAGTCCTCGGCCCACC
  TCTGCCCCAGCCATTGCTCAGAGTCAGGTCACAGAAGGGCCAGGGGCACCTATCAAAAAAACACCAAAGG
  AAGCTGTGAAGGTTGAAGAGAAGCGGGGAGAAGAGCCGGCTGAGCCCGCTGAGCCGGAGCCCACAGAAGC
  ATGGAAGGTGGAAAAAACCCACACCGAGGTCACAGTACCTACCTCAAATGGTGACCAAACGCAGAAGCTT
  GCAGGAAAAGGTGAAGATCTGATAAGAATGAGGAAGAAAAAGAGAGAGAGACTAGATGGTGAAAACATTT
  ATATCAGACATAGCAATTTAATGTTGGAGGATTTAGACAAGAGTCAAGAAGAGATCAAAAAGCACCATGC
  CAGCATCAGTGAACTGAAAAAGAACTTTATGGAATCGGTACCCGAACCACGGCCCAGCGAGTGGGACAAG
  CGCTTATCTACACACTCACCCTTCCGGACTCTTAACATCAACGGGCAAGTCCCTACTGGAGATGGACCTC
  CTCTGGTAAAGACTCAAACTGTCACCATCTCAGATACTGCCAATGCTGTGAAAAGTGAAATCCCAACCAA
  AGATGTCCCTATTGTCCACACTGAGACCAAGACCATCACCTATGAAGCTGCCCAGACTGAGGACAGCAAT
  GGGGACTTAGACCCTGGAGTCTTGCTGACAGCCCAGACCATCACATCCGAGACCACAAGTAGTACAACCA
  CGACACAGATTACCAAGACTGTAAAAGGTGGGATTTCTGAGACCCGGATCGAGAAGAGAATTGTGATCAC
  AGGAGATGCCGATATCGACCATGATCAGGTCCTTGTACAAGCCATCAAGGAAGCCAAGGAGCAGCATCCA
  GACATGTCAGTGACCAAGGTGGTCGTCCACCAGGAGACAGAGATCTCTGAGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_183428
• Insert Size: 2577 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183428.3, NP_906273.3
• RefSeq Size: 6565 bp
• RefSeq ORF: 2577 bp
• Locus ID: 269587
• UniProt ID: P48193
• Cytogenetics: 4 64.54 cM
• Gene Summary: Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript, The extent of this transcript is supported by transcript alignments.