Kmt5b (NM_144871) Mouse Untagged Clone

CAT#: MC222189

Suv420h1 (untagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kmt5b
• Synonyms: AA117471; C630029K18Rik; Suv4-20h1; Suv420h1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222189 representing NM_144871
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGGTTGGGAGACTCCAAGAACATGGTGGTGAATGGCAGGAGAAATGGAGGCAAGTTGTCTAATG
  ACCATCAGCAGAATCAATCAAAATTACAGCAGCACTCGGGCAAGGACACCCTGAAGACCGGCAGAAACGC
  CGTTGAGAGGCGGTCCAGCAGATGTCATGGTAACTCGGGATTTGAAGGGCAGAGCCGCTATGTGCCGTCC
  TCTGGAATGTCCGCCAAGGAGCTCTGTGAGAACGATGACTTAGCAACCAGTTTGGTTCTTGATCCCTACT
  TAGGTTTTCAGACACACAAAATGAACACTAGATTTCGGCCTATAAAAGGAAGGCAAGAAGAGCTAAAGGA
  AGTAATTGAACGCTTTAAGAAAGATGAACACTTAGAGAAAGCTTTCAAATGTTTGACTTCTGGGGAATGG
  GCACGGCATTATTTTCTCAACAAAAACAAAATGCAGGAGAAATTATTCAAGGAACATGTCTTTATTTACT
  TGCGGATGTTTGCAACTGACAGTGGATTTGAAATACTGCCTTGTAATAGATATTCTTCAGAACAAAATGG
  AGCCAAGATAGTTGCAACAAAAGAGTGGAAACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCC
  GAACTTTCAGAAATTGAGGAGAACATGCTACTTAGACACGGAGAAAACGACTTCAGTGTCATGTATTCCA
  CAAGGAAAAATTGTGCTCAACTCTGGCTCGGTCCTGCTGCATTTATAAATCATGATTGCAGACCTAACTG
  TAAGTTTGTGTCAACTGGTCGAGATACAGCATGCGTTAAGGCTCTGAGAGATATTGAACCTGGAGAAGAA
  ATTTCTTGTTACTATGGAGATGGCTTTTTTGGAGAAAATAATGAGTTCTGCGAATGTTATACTTGTGAAA
  GACGGGGAACTGGTGCTTTTAAATCACGAGTAGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATA
  CGGACTTAGAGAAACAGATAAACGCTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAACTCAGAC
  AGTCAGTCTGTCAGCTCTAACACAGATGCAGACACCACTCAGGAAAAAGACAATGCAACTTCTAATCGAA
  AATCTTCAGTTGGTGTGAAAAAGAGCAGCAAGAGTCGAGCTCTGACAAGGCCGTCCATGCCGAGAGTCCC
  GGCTGCTTCCAACTCTACCTCACCCAAGCTAGTGCACACCAACAATCCCCGGGTACCAAAGAAACTGAGA
  AAGCCGGCAAAGCCTTTACTCTCCAAGATCAGACTGCGGAATCACTGCAAGCGGCTGGACCAGAAGAGCG
  CATCCCGCAAGCTCGAGATGGGGAGCTTAGTGCTTAAGGAGCCCAAAGTCGTGCTATATAAAAATTTGCC
  AATTAAGAAAGAAAGGGAGCCAGAGGGACCAGCCCATGCTGCAGTGGGGAGTGGGTGCTTGACTAGACAT
  GCTGCGAGAGAACACAGGCAGAATCATGGGAGAGGTGCTCATTCGCAGGGCGACAGTTTGCCCTGCACCT
  ACACAACCCGGCGCTCTTTGAGGACAAGGACAGGTCTGAAGGAGACCACTGACATCAAGCTTGAACCAAG
  TCCCTTGGATGGCTATAAAAATGGTATACTGGAACCTTGCCCAGACAGTGGCCAGCAGCCAACCCCAGAG
  GTGCTGGAAGAACTGGCTCCTGAGACTGCACACAGGGAGGAAGCATCCCAGGAGTGTCCCAAGAACGACT
  CCTGCCTGTCACGAAAGAAATTTCGACAAGTGAAACCTGTGAAACACTTAGCAAAGACCGAGGACTGCAG
  TCCAGAGCACAGCTTCCCTGGGAAAGACGGGCTGCCAGATTTGCCAGGGTCTCATCCTGATCAAGGTGAG
  CCCAGTGGCACAGTCAGGGTGCCCGTGAGCCACACGGACTCTGCTCCCTCACCGGTTGGCTGCTCTGTTG
  TCGCACCCGACAGCTTCACAAAAGACAGCTTCAGAACTGCACAAAGTAAAAAGAAGCGGCGGGTCACCAG
  GTACGATGCACAGCTGATCCTGGAGAACAGCTCTGGAATCCCCAAGCTGACGCTTCGCAGGCGGCACGAC
  AGCAGCAGCAAGACAAACGACCATGAGAGTGACGGCGTGAACTCCTCCAAGATCAGCATCAAACTCAGCA
  AGGACCACGACAGTGACAGCAACCTCTATGTTGCCAAGCTCAGTAACGGGGTCAGCGCAGGGCCGGGCAG
  CAGCTCCACCAAGCTCAAGATCCAGCTCAAGCGGGATGAGGAGAGCAGGGGGCCATGTGCAGAGGGCCTG
  CACGAGAACGGGGTGTGCTGCAGCGACCCCCTCTCCCTGCTCGAGTCCCAGATGGAGGTGGACGACTACA
  GTCAATATGAGGAGGACAGCACAGATGAATCCTCATCTTCTGAGGGGGAGGAGGAGGAGGAGGACTGCGA
  GGATGACTTCGATGATGACTTCATTCCTCTTCCTCCGGCAAAGCGGCTGAGGCTAATTGTTGGTAAAGAC
  TCCATAGATATTGACATTTCTTCAAGGAGAAGAGAAGATCAGTCTCTAAGACTGAACGCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144871
• Insert Size: 2583 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144871.4, NP_659120.3
• RefSeq Size: 6062 bp
• RefSeq ORF: 2583 bp
• Locus ID: 225888
• UniProt ID: Q3U8K7
• Cytogenetics: 19 A
• Gene Summary: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 3 and 4 both encode isoform b.