Pde6a (NM_146086) Mouse Untagged Clone

CAT#: MC222188

Pde6a (untagged) - Mouse phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde6a
• Synonyms: nmf282; Pdea
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222188 representing NM_146086
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAGGTGACAGCAGAGGAGGTAGAAAAGTTCCTGGATTCAAATATTGGCTTTGCCAAACAGTACT
  ATAACTTTCACTACCGGGGGAAGGTCATCTCAGACCTCCTCGGGGCCAAGGAGGCAGCCGTGGACTTCAG
  CAACTACCACGATGTGAACAGCGTAGAGGAGAGTGAGATCATCTTTGACCTCCTGCGGGACGTTCAGGAG
  AACTTACAGGCTGAGAAATGCACATTCAATGTCATGAAGAAGCTCTGCTTCCTCCTGCGGGCTGACCGCA
  TGAGCCTGTTCATGTACAGGACCCGCAACGGGATCGCCGAGTTGGCCACTAGGCTCTTCAATGTCCACAA
  GGATGCCGTCCTAGAGGACTGCTTGGTGATGCCCGACTCCGAGATTGTCTTCCCTCTGGACATGGGTGTC
  GTGGGCCACGTCGCACACTCCAAAAAGATTGCCAATGTCCCCAACACAGAAGAGGATGAGCATTTCTGTG
  ACTTCGTGGACAATCTCACAGAATATCAGACCAAAAACATCCTGGCTTCCCCCATCATGAATGGGAAGGA
  TGTGGTAGCCATAATCATGGCTGTGAATAAAATAGATGAACCCCACTTCACCAAGAGAGATGAAGAGATT
  CTTCTCAAGTACCTCAACTTTGTGAACCTGATCATGAAGGTATTCCACCTGAGCTACCTGCACAACTGTG
  AGACTCGTCGCGGCCAGATATTGCTGTGGTCTGGGAGCAAGGTCTTTGAGGAGCTCACGGATATAGAGAG
  GCAGTTCCACAAGGCCCTGTACACGGTCCGGGCTTTCCTCAACTGTGACAGATACTCCGTAGGACTCTTA
  GACATGACCAAACAGAAGGAATTTTTTGATGTGTGGCCAGTTCTGATGGGCGAGGCTCCAGCTTACTCTG
  GTCCCAGGACTCCAGACGGAAGGGAAATTAACTTCTACAAGGTCATTGACTACATCCTGCACGGCAAAGA
  AGACATCAAAGTCATCCCGAACCCACCCGCTGACCACTGGGCTCTAGTGAGTGGTCTCCCCACTTACGTG
  GCTCAAAATGGTCTGATCTGCAATATAATGAATGCGCCTGCAGAGGACTTTTTTGAATTCCAGAAAGAGC
  CTCTGGATGAGTCTGGGTGGATGATTAAAAATGTACTCTCCATGCCCATCGTCAACAAGAAGGAAGAGAT
  CGTCGGCGTGGCCACATTTTACAACCGCAAAGATGGGAAGCCCTTCGACGATATGGACGAGACCCTCATG
  GAGTCTTTGACTCAGTTTCTGGGATGGTCAGTCTTAAACCCTGACACCTACGAGTCCATGAACAAGCTCG
  AGAACAGGAAGGATATCTTCCAGGACATCGTGAAATATCACGTGAAGTGTGATAACGAAGAAATCCAGAA
  GATCTTGAAAACCAGAGAGGTGTACGGCAAAGAGCCGTGGGAATGCGAGGAGGAGGAGCTGGCTGAGATC
  CTGCAAGGAGAACTTCCAGACGCGGAGTCATACGAAATCAACAAGTTCCACTTCAGCGACCTGCCACTCA
  CGGAGCTGGAGCTGGTGAAGTGCGGCATCCAGATGTACTACGAGCTCAGAGTGGTGGACAAGTTCCACAT
  CCCGCAAGAGGCCCTGGTGCGCTTCATGTATTCGCTAAGCAAAGGCTACCGGAGAATCACTTACCACAAC
  TGGCGGCATGGCTTCAACGTGGGGCAGACCATGTTCTCCTTGCTGGTGACAGGAAAGCTGAAACGGTACT
  TCACTGATCTAGAGGCCTTGGCCATGGTCACTGCTGCCTTCTGTCATGACATCGACCACAGAGGCACGAA
  CAACCTCTACCAGATGAAATCACAGAACCCCCTGGCCAAGCTCCATGGGTCCTCCATCTTGGAAAGGCAT
  CATTTGGAGTTTGGCAAAACACTCCTGAGAGATGAGAGCCTGAATATCTTCCAGAACCTGAATCGCCGGC
  AGCATGAGCACGCGATCCACATGATGGACATCGCGATCATTGCCACAGACCTTGCCTTGTATTTCAAGAA
  AAGGACCATGTTCCAGAAGATTGTGGATCAGTCAAAGACATATGAGAGTACCCAGGAGTGGACCCAGTAC
  ATGATGCTGGAGCAGACACGGAAGGAAATTGTGATGGCCATGATGATGACCGCCTGTGATCTCTCAGCCA
  TCACCAAACCCTGGGAGGTACAGAGCAAGGTGGCTCTGCTGGTGGCTGCTGAATTCTGGGAGCAAGGTGA
  CCTGGAGCGCACAGTGCTGCAGCAGAATCCCATTCCCATGATGGACAGAAACAAGGCGGATGAGCTCCCC
  AAGCTTCAAGTCGGCTTCATCGACTTTGTGTGCACTTTTGTCTATAAGGAGTTCTCCCGATTTCATGAGG
  AGATTACACCCATGCTGGATGGGATCACTAACAACCGCAAGGAATGGAAGGCGCTGGCTGATGAGTACGA
  AGCCAAGATGAAGGCCCTGGAGGAGGAGAAGCAGAAGCAGCAGGCAGCCAAGCAAGCTGCTTCCGGGAAC
  CAGCCAGGAGGGAACCCCACTCCAGGGGGTGCACCTGCATCTAAGTCCTGTTGCATCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146086
• Insert Size: 2583 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146086.2, NP_666198.1
• RefSeq Size: 4230 bp
• RefSeq ORF: 2583 bp
• Locus ID: 225600
• Cytogenetics: 18 34.41 cM