Ago3 (NM_153402) Mouse Untagged Clone

CAT#: MC222183

Ago3 (untagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ago3
• Synonyms: AW048688; C130014L07Rik; Eif2c3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222183 representing NM_153402
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCGGCTCCGCAGGACCCATTGGGGCCCAGCCCCTATTCATCGTGCCCAGAAGACCTGGGTATG
  GCACTATGGGCAAACCCATTAAACTGTTGGCTAACTGTTTTCAAGTTGAAATCCCAAAGATTGATGTCTA
  CCTCTATGAAGTTGACATCAAACCAGACAAGTGTCCTCGAAGAGTGAACAGAGAAGTAGTTGATTCAATG
  GTTCAGCATTTTAAAGTGACTATATTTGGAGACCGTAGACCAGTTTATGATGGAAAAAGAAGCCTTTATA
  CAGCCAATCCACTTCCTGTGGCAACCACTGGGGTAGATTTAGATGTTACATTACCTGGGGAAGGTGGAAA
  AGATCGACCTTTCAAGGTGTCAGTCAAATTTGTGTCTCGAGTCAGCTGGCACCTACTACATGAAGCACTG
  GCTGGAGGGACTTTGCCTGAGCCACTGGAATTAGACAAGCCTGTCAGCACTAACCCCGTCCATGCCGTTG
  ACGTGGTGCTCCGACATCTGCCCTCTATGAAATATACGCCTGTGGGGCGTTCCTTTTTCTCAGCTCCAGA
  AGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTATGGTTTGGATTCCATCAGTCTGTTCGGCCTGCC
  ATGTGGAAAATGATGCTAAATATTGATGTTTCTGCCACTGCCTTTTACAAAGCACAACCTGTAATTCAGT
  TCATGTGTGAAGTTCTTGACATTCACAACATTGATGAACAACCCAGACCCCTGACTGATTCTCATCGGGT
  AAAATTCACTAAGGAGATAAAAGGTCTGAAAGTTGAAGTGACTCATTGTGGAACAATGAGACGGAAATAC
  CGTGTTTGTAATGTAACGAGAAGGCCTGCCAGCCACCAAACCTTTCCTTTACAGCTAGAAAATGGCCAAA
  CTGTGGAGAGGACTGTGGCTCAGTATTTCAGAGAAAAGTACACGCTGCAGCTGAAGTACCCACACCTTCC
  CTGCCTGCAAGTGGGGCAGGAGCAGAAGCATACATACCTGCCACTAGAAGTCTGTAATATTGTGGCTGGG
  CAAAGGTGTATCAAGAAACTAACGGACAATCAGACTTCTACAATGATAAAGGCAACAGCAAGATCTGCAC
  CAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTGCAAATTATGAAACAGATCCATTTGTTCAGGA
  GTTTCAGTTTAAAGTGCGGGATGAAATGGCCCATGTGACAGGACGTGTACTTCCAGCACCTATGCTCCAG
  TACGGAGGACGGAACCGGACAGTAGCCACACCCAGCCACGGAGTATGGGACATGCGAGGAAAACAGTTCC
  ACACAGGAGTCGAAATCAAAATGTGGGCTATTGCTTGCTTTGCCACACAGAGGCAGTGCAGAGAAGAAAT
  ACTAAAAGGTTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCA
  TGCTTCTGCAAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACT
  CAGGACTGCAGCTCATTATCGTCATCCTGCCCGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTGGG
  AGATACACTTTTGGGTATGGCCACACAGTGTGTACAAGTCAAGAATGTAATAAAAACATCTCCTCAAACT
  CTTTCAAACTTGTGCCTAAAGATCAATGTTAAACTTGGAGGAATCAATAATATTCTTGTACCTCATCAAA
  GACCTTCTGTGTTCCAGCAACCAGTGATCTTCCTGGGCGCAGATGTCACACACCCGCCTGCTGGTGACGG
  AAAAAAGCCCTCCATTGCTGCTGTTGTAGGTAGTATGGATGCCCATCCAAGCAGATACTGTGCCACAGTA
  AGAGTTCAGAGACCCCGACAGGAAATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTGCTCATCCAAT
  TTTATAAATCAACCCGATTCAAACCTACTCGTATAATCTTCTATCGGGATGGTGTTTCAGAAGGACAGTT
  CAGACAGGTATTATATTATGAGCTACTGGCAATTCGAGAAGCCTGTATCAGTTTGGAAAAAGACTATCAA
  CCTGGAATAACCTACATTGTTGTTCAGAAGAGACATCATACACGGTTGTTCTGTGCTGATAGGACAGAAA
  GGGTTGGAAGAAGCGGCAACATCCCAGCTGGAACCACGGTGGATACCGACATTACTCACCCATATGAATT
  CGACTTTTATCTCTGTAGCCATGCTGGAATACAGGGTACCAGCCGTCCTTCACACTATCATGTTTTATGG
  GATGACAACTTCTTTACTGCAGATGAACTTCAGCTGCTCACTTACCAGCTCTGTCACACTTACGTACGCT
  GTACAAGATCTGTTTCTATCCCTGCACCAGCATATTACGCTCACCTGGTGGCATTCAGAGCCAGATATCA
  TCTTGTGGACAAGGAACATGACAGTGCTGAAGGAAGCCATGTTTCAGGACAGAGCAATGGGCGAGATCCA
  CAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACGCACAATGTACTTCGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153402
• Insert Size: 2583 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153402.2, NP_700451.2
• RefSeq Size: 7635 bp
• RefSeq ORF: 2583 bp
• Locus ID: 214150
• UniProt ID: Q8CJF9
• Cytogenetics: 4 D2.2
• Gene Summary: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4 (PubMed:19174539). Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (By similarity).[UniProtKB/Swiss-Prot Function]