Kctd1 (NM_001142731) Mouse Untagged Clone

CAT#: MC222174

Kctd1 (untagged) - Mouse potassium channel tetramerisation domain containing 1 (Kctd1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kctd1
• Synonyms: 4933402K10Rik; AI661543; AW553000
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222174 representing NM_001142731
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGAATGCCTGGCAGCGGGGACTGTAACACCAGCGCGGGCGGCAGCGCCGCTGCTGCGGCCGAGA
  ACAATGGGGATCGGGGTGAGGGTGAGCGCGGGGCCGGGGGCCGCGGCCGCCGCTACGGCCGCCCGCATTA
  CTGCAGCGCGGGCGAGGAGGAGGAGGAGGAAGAGGAGGAGGACGAGATCCAGGAGGTGCAGATAACGGGG
  GACGAGGAGGAGGACGGAGGTGGGGGGCTGGAGGAGGACGAGGAGGAGGAGGAAGAGGAGGAGATGGGGA
  TGGACTGGGAAGAGCCCCTGGAGCCGGAGGACTCGGCCGGGGAGGAGCTGGAGCCGGAGCCGGTCCATAT
  GATCCATATGGACCAGAGCGCCGCGCTGGAGCCCGAGGCGCCGCCACGACTGTTGGCGCCCCGGGCCCGC
  GCGGGGCCGCCGGGGGACAGCGCGGAGCTGGACCCCGACGTGCTGCAGCGCCCCGAGCGGGCCCGGCTGA
  GCGAGAACACCAGGCTGGCCACCCGCTACGCCGTGCGCATCTTCCGGGAGTACCTGAGCGAAAAGGCTCA
  GAGCCCGGACTTCGAGACCATGGACAAGGGGGCGCTGTGCCGCGTGCTGCGCTCCTTCTATGCCGAGGCC
  CGTTCTAAGAGCGGCCAGCTCTACAGCAAGTCCTCGCTCATCAGTATCCGCAGCTCCCTCAACCGCTACC
  TCAACGAGCCCCCTTACTGTCGCACGCTCGACCTCACCAAGGACCCCGAGCTGCGCAGCGCCAACCTGAC
  GCTGGCCGCAGTCATCCGCAAGCTCGAGGAGCAGGGCGCGGGGCCCGTGGTGCAGAAGCAAGCCATCACG
  CGCGCCGACCTGCGCAAGCTCTACACCTCCAGCGTCTTCAGCACCAACACGCCCTTTGGGCTGCTCAACA
  AGGTCTGGTTCGAGACGTGCATGTACTTCTGCACGCGAGGCCGTGAGAACCAGCGCGAGCTGGAGGAGGA
  CTCCTTCGGGCTGGCCATGGACGAGGACGGTCGCAAGTTCGTTTACTTCAAGTCGCTTGGGCCTTACCAT
  AAGTCGCGTTCCTCGTCCTGGAGTAAGAAGCGTGCGGAGAGCAGCGACGAAGAGAACTTGCCTCGCATGT
  ACGAGACGGGCACTGAGTTCTGTCCCTACGCCAGCTTTGTCAAGTACCTGTCTAAGCGCAATCCCCTCTG
  TAAGGCGTTTTTCCAGCGGCCCCGGGACCACTGCAGTGAGGGCGATGTGACATGGTACGAGAACAAAGCC
  ATAGGCAAGAACTTGCTGGGCACCAGGATGCAGATGCTGTCCAAGGCAGCCAAGCTGTCCAAGACCTACA
  CTAACCATTGCATCGGCGCGGTGTCCATCGCCACACTCAACAGCATCGCGGGTATCGGGACCAAGCTAGG
  CTCCCCAGCCCCGCAGGGCTGCTATACCGACTCTCTGAACGGGTCAGCTCGTCACCACTCCCACCACGCT
  CCCACCCATCCTTCCCACCACCACCGCCCCCAGCCGCCCTCGCTGGGTAACACTTATATCCTCCCCAAAG
  ACAGCCAGGTCGGGCCCGACGTGAAGTCCGAGGCGGCGCCCAAGCGCACGCTGTACGAGTCAGTGTTCGG
  GTCAGGGGAAATCTGCGGCCCTTCTTCCCCCAAAAGACTTTGTATCCGTCCCTCCTCGGAACCTGTGGAT
  GCGGTGGTGGTGGTTTCCGTGAAACACGACCCCCTGCCTCTTCTTCCAGAAGTCAATGGGCACCGAAGCA
  CCAATTCTCCCACCATAGTTTCACCTGCTATTGTTTCCCCCGCCCAGGACAGTCGGCCCAATATGTCAAG
  ACCTCTGATCACTCGATCCCCTGCATCGCCACTGAACAACCAAGGCATTCCAACTCCAGCTCAGCTCACC
  AAATCCAACGCACCCGTCCACATCGACGTGGGCGGCCACATGTACACCAGCAGCCTGGCCACCCTCACCA
  AATACCCCGAATCCAGAATCGGAAGACTCTTTGATGGTACAGAGCCCATTGTTCTGGACAGTCTCAAACA
  GCACTATTTCATTGACAGAGACGGGCAGATGTTCAGATATATCTTGAATTTTCTACGAACATCAAAACTC
  CTCATTCCTGATGATTTCAAGGACTATACTTTGTTATATGAAGAGGCAAAATACTTTCAACTTCAGCCAA
  TGTTGTTGGAGATGGAGCGATGGAAACAGGACAGGGAAACTGGCCGCTTTTCACGGCCCTGCGAGTGCCT
  CGTGGTGCGTGTGGCCCCGGACCTTGGAGAAAGGATCACCCTCAGTGGGGACAAGTCCTTGATAGAGGAA
  GTATTTCCCGAGATCGGCGATGTGATGTGCAACTCGGTCAATGCAGGCTGGAATCACGACTCAACACATG
  TCATCAGGTTTCCACTAAACGGCTACTGCCACCTCAATTCAGTTCAGGTCCTTGAGCGGTTGCAGCAAAG
  AGGATTTGAAATTGTGGGTTCCTGTGGAGGAGGAGTGGACTCGTCCCAGTTCAGTGAATATGTCCTCCGA
  CGGGAACTGAGGCGGACACCCCGAGTACCCTCAGTCATCCGGATAAAACAGGAGCCACTGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142731
• Insert Size: 2586 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142731.1, NP_001136203.1
• RefSeq Size: 3456 bp
• RefSeq ORF: 2586 bp
• Locus ID: 106931
• Cytogenetics: 18 A1
• Gene Summary: May repress the transcriptional activity of AP-2 family members, including TFAP2A, TFAP2B and TFAP2C to various extent.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.